Retrieval of TGN proteins from the cell surface requires endosomal acidification

• Published in: The EMBO journal Vol. 13; no. 10; pp. 2305 - 2312
• Main Authors: Chapman, R.E., Munro, S.
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group 15.05.1994
• Subjects: Acids - metabolism; Amino Acid Sequence; Animals; Biological and medical sciences; Biological Transport - drug effects; Cell Compartmentation; Cell Membrane - metabolism; Cell physiology; Chloroquine - pharmacology; Endocytosis - drug effects; Endocytosis - physiology; Fluorescent Antibody Technique; Fundamental and applied biological sciences. Psychology; Furin; Glycoproteins; Golgi Apparatus - enzymology; Golgi Apparatus - metabolism; Membrane and intracellular transports; Membrane Glycoproteins - metabolism; Membrane Proteins; Molecular and cellular biology; Molecular Sequence Data; Organelles - metabolism; Subtilisins - metabolism; Proteins; Vertebrata; Endocytosis; Cell line; Mammalia; Rat; Rodentia; pH; Molecular targeting; Endosome; Kidney; Golgi apparatus

TGN38 is a protein of unknown function located in the trans‐Golgi network (TGN) of mammalian cells. Its intracellular distribution is maintained by it being continuously retrieved from the plasma membrane. In this paper we show that when cells are treated with agents such as chloroquine which neutra...