Evaluation of endoscope sheaths as viral barriers

• Published in: The Laryngoscope Vol. 109; no. 4; p. 636
• Main Authors: Baker, K H, Chaput, M P, Clavet, C R, Varney, G W, To, T M, Lytle, C D
• Format: Journal Article
• Language: English
• Published: United States 01.04.1999
• Subjects: Disinfection - methods; Endoscopes - virology; Equipment Contamination - prevention & control; Evaluation Studies as Topic; Laryngoscopes
• ISSN: 0023-852X
• DOI: 10.1097/00005537-199904000-00022

Evaluate ENT endoscope sheaths as barriers to virus passage. "Defective" sheaths covering an endoscope were challenged with virus to determine how many virus particles could be recovered from the endoscope. Sheaths with small laser-drilled holes (2 to 30 microm) were challenged with high-titer virus suspensions (10(8) viruses/mL). The inside of the sheath and the endoscope were separately rinsed to recover any virus that penetrated through the hole in the sheath. In an attempt to assess the possible importance of holes in the sheaths, a sequential test was conducted with an initial virus challenge outside a defective sheath (30-micron hole in the sheath), after which the possibly contaminated endoscope was removed and inserted into a second defective sheath (with a 20-micron hole at the same location) to determine whether the contaminating virus would pass outward through the second sheath. Small volumes of virus-containing fluid penetrated through the hole, e.g., 500 virus particles passed through one of three 30-microm holes. A significant fraction of those virus particles was occasionally found on the endoscope after removal from the sheath. Similar results were obtained with sheaths that had small tears (34-84 microm in length, from punctures with fine wires). Although some virus penetration could occur during the initial challenge contaminating the endoscope, no virus was detected passing outward through the second sheath. Use of a sheath combined with intermediate level disinfection should provide a safe instrument for ENT endoscopy.