An adenine-to-guanine nucleotide change in the IRES SL-IV domain of picornavirus/hepatitis C chimeric viruses leads to a nonviable phenotype

• Published in: Virology (New York, N.Y.) Vol. 317; no. 2; pp. 345 - 358
• Main Authors: McKnight, Kevin L, Sandefur, Stephanie, Phipps, Krista M, Heinz, Beverly A
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 20.12.2003
• Subjects: Adenine - chemistry; Animals; Base Sequence; Cattle; Chimeric viruses; Gene Expression Regulation, Viral; Guanine - chemistry; HeLa Cells; Hepacivirus - chemistry; Hepacivirus - genetics; Hepacivirus - physiology; Hepatitis C virus; Humans; Internal ribosomal entry site; Molecular Sequence Data; Mutation; Nucleic Acid Conformation; Picornaviridae - chemistry; Picornaviridae - genetics; Picornaviridae - physiology; Poliovirus - chemistry; Poliovirus - genetics; Poliovirus - physiology; Protein Biosynthesis; Recombination, Genetic; Ribosomes - metabolism; RNA structure; RNA, Viral - chemistry; RNA, Viral - genetics; Transcription, Genetic; Transfection; Translation; Viral Plaque Assay; Internal ribosomal entry site; Translation; Hepatitis C virus; RNA structure; Chimeric viruses

The inability for the internal ribosomal entry site (IRES) of hepatitis C virus (HCV) to be readily studied in the context of viral replication has been circumvented by constructing chimeras such as with poliovirus (PV), in which translation of the genome polyprotein is under control of the HCV IRES...