Non-invasive, transdermal, path-selective and specific glucose monitoring via a graphene-based platform

• Published in: Nature nanotechnology Vol. 13; no. 6; pp. 504 - 511
• Main Authors: Lipani, Luca, Dupont, Bertrand G. R., Doungmene, Floriant, Marken, Frank, Tyrrell, Rex M., Guy, Richard H., Ilie, Adelina
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 01.06.2018; Nature Publishing Group
• Subjects: 631/61/350/59; 639/166/985; 639/301/1005/1009; 639/925/352/1060; 639/925/918; Animals; Arrays; Biosensing Techniques - instrumentation; Blood; Blood Glucose Self-Monitoring - instrumentation; Chemistry and Materials Science; Electroosmosis; Equipment Design; Extracellular Fluid - chemistry; Fluxes; Glucose; Glucose - analysis; Glucose monitoring; Graphene; Graphite - chemistry; Humans; Materials Science; Monitoring; Nanotechnology; Nanotechnology and Microengineering; Pixels; Skin; Skin - chemistry; Sugar; Swine; Thin films
• DOI: 10.1038/s41565-018-0112-4
• ISSN: 1748-3387; 1748-3395

Currently, there is no available needle-free approach for diabetics to monitor glucose levels in the interstitial fluid. Here, we report a path-selective, non-invasive, transdermal glucose monitoring system based on a miniaturized pixel array platform (realized either by graphene-based thin-film technology, or screen-printing). The system samples glucose from the interstitial fluid via electroosmotic extraction through individual, privileged, follicular pathways in the skin, accessible via the pixels of the array. A proof of principle using mammalian skin ex vivo is demonstrated for specific and ‘quantized’ glucose extraction/detection via follicular pathways, and across the hypo- to hyper-glycaemic range in humans. Furthermore, the quantification of follicular and non-follicular glucose extraction fluxes is clearly shown. In vivo continuous monitoring of interstitial fluid-borne glucose with the pixel array was able to track blood sugar in healthy human subjects. This approach paves the way to clinically relevant glucose detection in diabetics without the need for invasive, finger-stick blood sampling. A non-invasive, transdermal monitor extracts and detects glucose from the interstitial fluid through individual, privileged, follicular pathways in the skin accessible via a miniaturized, graphene-supported pixel array.