Single-Cell Analysis of Diverse Pathogen Responses Defines a Molecular Roadmap for Generating Antigen-Specific Immunity

• Published in: Cell systems Vol. 8; no. 2; pp. 109 - 121.e6
• Main Authors: Blecher-Gonen, Ronnie, Bost, Pierre, Hilligan, Kerry L., David, Eyal, Salame, Tomer Meir, Roussel, Elsa, Connor, Lisa M., Mayer, Johannes U., Bahar Halpern, Keren, Tóth, Beáta, Itzkovitz, Shalev, Schwikowski, Benno, Ronchese, Franca, Amit, Ido
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 27.02.2019; Elsevier
• Subjects: Animals; antigen-presenting cells; cell-cell communication; dendritic cells; Human health and pathology; Humans; IFN-γ; Immunity, Innate; Immunity, Innate - immunology; Immunology; Infectious diseases; innate immune cells; Life Sciences; lymph node; Mice; monocytes; NK cells; Single-Cell Analysis; Single-Cell Analysis - methods; single-cell RNA-seq; T cell polarization; monocytes; NK cells; IFN-γ; dendritic cells; antigen-presenting cells; innate immune cells; lymph node; T cell polarization; single-cell RNA-seq; cell-cell communication

The immune system generates pathogen-tailored responses. The precise innate immune cell types and pathways that direct robust adaptive immune responses have not been fully characterized. By using fluorescent pathogens combined with massively parallel single-cell RNA-seq, we comprehensively character...