Influence of WNK3 on intracellular chloride concentration and volume regulation in HEK293 cells

• Published in: Pflügers Archiv Vol. 464; no. 3; pp. 317 - 330
• Main Authors: Cruz-Rangel, Silvia, Gamba, Gerardo, Ramos-Mandujano, Gerardo, Pasantes-Morales, Herminia
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer-Verlag 01.09.2012; Springer Nature B.V
• Subjects: Biomedical and Life Sciences; Biomedicine; Cell Biology; Cell Size; Chlorides - metabolism; Cytosol - metabolism; HEK293 Cells; Human Physiology; Humans; K Cl- Cotransporters; Molecular Medicine; Mutation; Neurosciences; Osmolar Concentration; Protein Serine-Threonine Kinases - genetics; Protein Serine-Threonine Kinases - metabolism; Receptors; Signaling and Cell Physiology; Sodium-Potassium-Chloride Symporters - metabolism; Symporters - metabolism; Taurine - metabolism; RVD; SPAK; RVI; Osmolarity; Kinase; NKCC; KCC

The involvement of WNK3 (with no lysine [K] kinase) in cell volume regulation evoked by anisotonic conditions was investigated in two modified stable lines of HEK293 cells: WNK3+, overexpressing WNK3 and WNK3-KD expressing a kinase inactive by a punctual mutation (D294A) at the catalytic site. This...