Engineering of β-mannanase from Aspergillus niger to increase product selectivity towards medium chain length mannooligosaccharides

• Published in: Journal of bioscience and bioengineering Vol. 130; no. 5; pp. 443 - 449
• Main Authors: Arunrattanamook, Nattapol, Wansuksri, Rungtiva, Uengwetwanit, Tanaporn, Champreda, Verawat
• Format: Journal Article
• Language: English
• Published: Elsevier B.V 01.11.2020
• Subjects: anion exchange chromatography; Aspergillus niger; Enzyme engineering; hydrolysis; Mannooligosaccharide; molecular weight; oligosaccharides; Prebiotics; Sugar profile; β-Mannanase; Mannooligosaccharide; Enzyme engineering; β-Mannanase; Sugar profile; Prebiotics

Mannooligosaccharides (MOSs) are one of the most commonly used biomass-derived feed additives. The effectiveness of MOS varies with the length of oligosaccharides, medium length MOSs such as mannotetraose and mannopentaose being the most efficient. This study aims at improving specificity of β-manna...