miR-22 inhibits the proliferation, motility, and invasion of human glioblastoma cells by directly targeting SIRT1

• Published in: Tumor biology Vol. 37; no. 5; pp. 6761 - 6768
• Main Authors: Chen, Hanchun, Lu, Qiong, Fei, Xifeng, Shen, Likui, Jiang, Dongyi, Dai, Dongwei
• Format: Journal Article
• Language: English
• Published: Dordrecht Springer Netherlands 01.05.2016; Springer Nature B.V
• Subjects: 3' Untranslated Regions; Biomedical and Life Sciences; Biomedicine; Cancer; Cancer Research; Cell growth; Cell Line, Tumor; Cell Movement; Cell Proliferation; Gene Expression Profiling; Gene Expression Regulation, Neoplastic; Glioblastoma - genetics; Glioblastoma - mortality; Glioblastoma - pathology; Humans; MicroRNAs; MicroRNAs - chemistry; MicroRNAs - genetics; Nervous system; Original Article; RNA Interference; RNA, Messenger - chemistry; RNA, Messenger - genetics; Sirtuin 1 - chemistry; Sirtuin 1 - genetics; Transcriptome; SIRT1; miR-22; Glioblastoma
• DOI: 10.1007/s13277-015-4575-8
• ISSN: 1010-4283; 1423-0380

Recently, microRNAs (miRNAs), a kind of small and non-coding RNA, can target the downstream molecules. Increasing evidence demonstrates that miRNAs meditate the onset and progression of a variety of tumors. In the present study, we carried out gene transfection, western blot, and reverse transcription PCR (RT-PCR) to explore the role of miR-22 in glioblastoma tissues and cell lines. Here, we verified that the expression of miR-22 was downregulated in glioblastoma tissues and cells rather than matched non-tumor tissues and normal human astrocyte (NHA) cells ( p  < 0.001). By contrast, SIRT1 messenger RNA (mRNA) and protein were upregulated in glioblastoma tissues and cells ( p  < 0.001). In vitro miR-22 mimics interfered with cell proliferation, migration, and invasion of U87 and U251 cells. Mechanically, the 3′-untranslated regions (3′-UTRs) of SIRT1 were a direct target of miR-22, leading to the decreased expression of SIRT1 protein in U87 and U251 cells. Meanwhile, miR-22 mimics also inhibited the expression of epidermal growth factor receptor (EGFR) and matrix metallopeptidase 9 (MMP9). In conclusion, miR-22 inhibited cell proliferation, migration, and invasion via targeting the 3′-UTR of SIRT1 in the progression of glioblastoma and miR-22-SIRT1 pathway can be recommended as a potential target for treatment of glioblastoma.