Probing conformational dynamics in biomolecules via chemical exchange saturation transfer: a primer

• Published in: Journal of biomolecular NMR Vol. 67; no. 4; pp. 243 - 271
• Main Authors: Vallurupalli, Pramodh, Sekhar, Ashok, Yuwen, Tairan, Kay, Lewis E.
• Format: Journal Article
• Language: English
• Published: Dordrecht Springer Netherlands 01.04.2017; Springer Nature B.V
• Subjects: Biochemistry; Biological and Medical Physics; Biophysics; Biopolymers - chemistry; Magma; Magnetic fields; Magnetization; Molecular Conformation; Molecular Dynamics Simulation; NMR; Nuclear magnetic resonance; Nuclear Magnetic Resonance, Biomolecular - methods; Perspective; Physics; Physics and Astronomy; Proteins - chemistry; Ribonucleic acid; RNA; RNA - chemistry; Spectroscopy/Spectrometry; Molecular dynamics; Slow conformational exchange; Excited conformational states; CEST

Although Chemical Exchange Saturation Transfer (CEST) type NMR experiments have been used to study chemical exchange processes in molecules since the early 1960s, there has been renewed interest in the past several years in using this approach to study biomolecular conformational dynamics. The metho...