Derivation and characterization of human embryonic stem cells on human amnion epithelial cells

Culture conditions that support the growth of undifferentiated human embryonic stem cells (hESCs) have already been established using primary human amnion epithelial cells (hAECs) as an alternative to traditional mitotically inactivated mouse embryonic fibroblasts (MEFs). In the present work, inner...

Full description

Saved in:
Bibliographic Details
Published inScientific reports Vol. 5; no. 1; p. 10014
Main Authors Lai, Dongmei, Wang, Yongwei, Sun, Jian, Chen, Yifei, Li, Ting, Wu, Yi, Guo, Lihe, Wei, Chunsheng
Format Journal Article
LanguageEnglish
Published London Nature Publishing Group UK 07.05.2015
Nature Publishing Group
Subjects
13
13/1
13/100
13/106
13/51
14
14/105
14/28
45
631/532
631/532/2117
Amnion
Amnion - cytology
Biomarkers
Blastocyst - cytology
Cell culture
Cell Culture Techniques
Cell Differentiation
Cell Line
Cell lines
Chromosome Banding
Cytoplasm
Electron microscopy
Embryo cells
Embryo fibroblasts
Embryonic Stem Cells - cytology
Embryonic Stem Cells - metabolism
Embryonic Stem Cells - ultrastructure
Embryos
Epithelial cells
Feeder Cells
Gene Expression Profiling
Gene Expression Regulation, Developmental
Humanities and Social Sciences
Humans
In vitro fertilization
multidisciplinary
Pluripotency
Pluripotent Stem Cells - cytology
Pluripotent Stem Cells - metabolism
Pluripotent Stem Cells - ultrastructure
Science
Stem cell transplantation
Stem cells
Teratoma
Transmission electron microscopy
Online AccessGet full text

Cover

Abstract Culture conditions that support the growth of undifferentiated human embryonic stem cells (hESCs) have already been established using primary human amnion epithelial cells (hAECs) as an alternative to traditional mitotically inactivated mouse embryonic fibroblasts (MEFs). In the present work, inner cell masses (ICM) were isolated from frozen embryos obtained as donations from couples undergoing in vitro fertilization (IVF) treatment and four new hESC lines were derived using hAECs as feeder cells. This feeder system was able to support continuous growth of what were, according to their domed shape and markers, undifferentiated naïve-like hESCs. Their pluripotent potential were also demonstrated by embryoid bodies developing to the expected three germ layers in vitro and the productions of teratoma in vivo . The cell lines retained their karyotypic integrity for over 35 passages. Transmission electron microscopy (TEM) indicated that these newly derived hESCs consisted mostly of undifferentiated cells with large nuclei and scanty cytoplasm. The new hESCs cultured on hAECs showed distinct undifferentiated characteristics in comparison to hESCs of the same passage maintained on MEFs. This type of optimized culture system may provide a useful platform for establishing clinical-grade hESCs and assessing the undifferentiated potential of hESCs.
AbstractList Culture conditions that support the growth of undifferentiated human embryonic stem cells (hESCs) have already been established using primary human amnion epithelial cells (hAECs) as an alternative to traditional mitotically inactivated mouse embryonic fibroblasts (MEFs). In the present work, inner cell masses (ICM) were isolated from frozen embryos obtained as donations from couples undergoing in vitro fertilization (IVF) treatment and four new hESC lines were derived using hAECs as feeder cells. This feeder system was able to support continuous growth of what were, according to their domed shape and markers, undifferentiated naïve-like hESCs. Their pluripotent potential were also demonstrated by embryoid bodies developing to the expected three germ layers in vitro and the productions of teratoma in vivo . The cell lines retained their karyotypic integrity for over 35 passages. Transmission electron microscopy (TEM) indicated that these newly derived hESCs consisted mostly of undifferentiated cells with large nuclei and scanty cytoplasm. The new hESCs cultured on hAECs showed distinct undifferentiated characteristics in comparison to hESCs of the same passage maintained on MEFs. This type of optimized culture system may provide a useful platform for establishing clinical-grade hESCs and assessing the undifferentiated potential of hESCs.
Culture conditions that support the growth of undifferentiated human embryonic stem cells (hESCs) have already been established using primary human amnion epithelial cells (hAECs) as an alternative to traditional mitotically inactivated mouse embryonic fibroblasts (MEFs). In the present work, inner cell masses (ICM) were isolated from frozen embryos obtained as donations from couples undergoing in vitro fertilization (IVF) treatment and four new hESC lines were derived using hAECs as feeder cells. This feeder system was able to support continuous growth of what were, according to their domed shape and markers, undifferentiated naïve-like hESCs. Their pluripotent potential were also demonstrated by embryoid bodies developing to the expected three germ layers in vitro and the productions of teratoma in vivo. The cell lines retained their karyotypic integrity for over 35 passages. Transmission electron microscopy (TEM) indicated that these newly derived hESCs consisted mostly of undifferentiated cells with large nuclei and scanty cytoplasm. The new hESCs cultured on hAECs showed distinct undifferentiated characteristics in comparison to hESCs of the same passage maintained on MEFs. This type of optimized culture system may provide a useful platform for establishing clinical-grade hESCs and assessing the undifferentiated potential of hESCs.
Abstract Culture conditions that support the growth of undifferentiated human embryonic stem cells (hESCs) have already been established using primary human amnion epithelial cells (hAECs) as an alternative to traditional mitotically inactivated mouse embryonic fibroblasts (MEFs). In the present work, inner cell masses (ICM) were isolated from frozen embryos obtained as donations from couples undergoing in vitro fertilization (IVF) treatment and four new hESC lines were derived using hAECs as feeder cells. This feeder system was able to support continuous growth of what were, according to their domed shape and markers, undifferentiated naïve-like hESCs. Their pluripotent potential were also demonstrated by embryoid bodies developing to the expected three germ layers in vitro and the productions of teratoma in vivo . The cell lines retained their karyotypic integrity for over 35 passages. Transmission electron microscopy (TEM) indicated that these newly derived hESCs consisted mostly of undifferentiated cells with large nuclei and scanty cytoplasm. The new hESCs cultured on hAECs showed distinct undifferentiated characteristics in comparison to hESCs of the same passage maintained on MEFs. This type of optimized culture system may provide a useful platform for establishing clinical-grade hESCs and assessing the undifferentiated potential of hESCs.
ArticleNumber 10014
Author Wang, Yongwei
Lai, Dongmei
Li, Ting
Wu, Yi
Guo, Lihe
Wei, Chunsheng
Sun, Jian
Chen, Yifei
Author_xml – sequence: 1
  givenname: Dongmei
  surname: Lai
  fullname: Lai, Dongmei
  organization: The International Peace Maternity and Child Health Hospital, School of medicine, Shanghai Jiaotong University
– sequence: 2
  givenname: Yongwei
  surname: Wang
  fullname: Wang, Yongwei
  organization: The International Peace Maternity and Child Health Hospital, School of medicine, Shanghai Jiaotong University
– sequence: 3
  givenname: Jian
  surname: Sun
  fullname: Sun, Jian
  organization: The International Peace Maternity and Child Health Hospital, School of medicine, Shanghai Jiaotong University
– sequence: 4
  givenname: Yifei
  surname: Chen
  fullname: Chen, Yifei
  organization: The International Peace Maternity and Child Health Hospital, School of medicine, Shanghai Jiaotong University
– sequence: 5
  givenname: Ting
  surname: Li
  fullname: Li, Ting
  organization: The International Peace Maternity and Child Health Hospital, School of medicine, Shanghai Jiaotong University
– sequence: 6
  givenname: Yi
  surname: Wu
  fullname: Wu, Yi
  organization: The International Peace Maternity and Child Health Hospital, School of medicine, Shanghai Jiaotong University
– sequence: 7
  givenname: Lihe
  surname: Guo
  fullname: Guo, Lihe
  organization: The International Peace Maternity and Child Health Hospital, School of medicine, Shanghai Jiaotong University
– sequence: 8
  givenname: Chunsheng
  surname: Wei
  fullname: Wei, Chunsheng
  organization: Eye and ENT Hospital, Fudan University
BackLink https://www.ncbi.nlm.nih.gov/pubmed/25950719$$D View this record in MEDLINE/PubMed
BookMark eNplkd9LwzAQx4NM3Jx78B-Qgk8K06RJ2vRFkPkTBr7oqyFLr1tHm8ykHcy_3ozOMfEgXLjvh-8dd6eoZ6wBhM4JviGYilvvYEUwJuwIDWLM-Dimcdw7-PfRyPslDsHjjJHsBPVjnnGckmyAPh_AlWvVlNZEyuSRXiindBOK313RFtGirZWJoJ65jTWljnwDdaShqnwUgE5VtdnSsCqbBVSlqjrgDB0XqvIw2uUh-nh6fJ-8jKdvz6-T--lYMyqaccJ1wnIlMGcCU6YpV3mcFBkBkjCidZHkBeV5DoKLguYwE2lKBNCUs1QkjNIhuut8V-2shlyDaZyq5MqVtXIbaVUp_yqmXMi5XUvGYrp9Q3S5M3D2qwXfyKVtnQkzSyKyjLGUYxaoq47Szvqw-GLfgWC5vYbcXyOwF4cj7cnf3QfgugN8kMwc3EHLf24_iAGW0Q
CitedBy_id crossref_primary_10_1007_s12015_018_9862_5
crossref_primary_10_1021_acsbiomaterials_2c00650
crossref_primary_10_1186_s42269_022_00705_3
crossref_primary_10_1016_j_yexcr_2019_03_028
crossref_primary_10_1007_s00418_020_01940_3
crossref_primary_10_3389_fvets_2021_706106
crossref_primary_10_1021_acsbiomaterials_0c01462
crossref_primary_10_1007_s12015_022_10438_5
crossref_primary_10_1177_0022034515611602
crossref_primary_10_3390_cells13070628
crossref_primary_10_1007_s10815_023_02773_4
crossref_primary_10_1093_abbs_gmw090
crossref_primary_10_3892_mmr_2017_6795
crossref_primary_10_1002_dvdy_24405
Cites_doi 10.1016/j.ydbio.2003.12.034
10.1126/science.1151526
10.1016/j.stem.2008.07.001
10.1016/j.stem.2009.05.015
10.1054/tice.1999.0087
10.1634/stemcells.2004-0201
10.1007/978-1-61779-794-1_6
10.1007/s004360050066
10.1038/nature05972
10.1093/humrep/deg290
10.1634/stemcells.2006-0053
10.1089/scd.2012.0325
10.1634/stemcells.2004-0357
10.1089/cell.2011.0068
10.1038/nature12745
10.1016/j.stem.2014.07.002
10.1073/pnas.78.12.7634
10.1186/1476-4598-7-76
10.1016/S1472-6483(10)61916-5
10.1089/clo.2008.0047
10.1073/pnas.0712136105
10.1634/stemcells.22-3-367
10.1126/science.282.5391.1145
10.1007/s13238-012-2007-8
10.1038/nature05950
10.1016/S1472-6483(10)61873-1
10.1038/ng1760
10.1038/292154a0
10.1095/biolreprod.104.033480
10.1038/74447
10.1038/nm1181
10.1073/pnas.1004584107
10.1038/nature08562
10.1089/cell.2009.0106
10.4161/cc.8.6.7934
10.1093/nar/gkp1071
10.1634/stemcells.21-2-131
10.1017/S1462399405009804
10.1111/j.0021-8782.2004.00336.x
10.1261/rna.1368009
10.1634/stemcells.21-5-546
ContentType Journal Article
Copyright The Author(s) 2015
Copyright Nature Publishing Group May 2015
Copyright © 2015, Macmillan Publishers Limited 2015 Macmillan Publishers Limited
Copyright_xml – notice: The Author(s) 2015
– notice: Copyright Nature Publishing Group May 2015
– notice: Copyright © 2015, Macmillan Publishers Limited 2015 Macmillan Publishers Limited
DBID C6C
CGR
CUY
CVF
ECM
EIF
NPM
AAYXX
CITATION
3V.
7X7
7XB
88A
88E
88I
8FE
8FH
8FI
8FJ
8FK
ABUWG
AFKRA
AZQEC
BBNVY
BENPR
BHPHI
CCPQU
DWQXO
FYUFA
GHDGH
GNUQQ
HCIFZ
K9.
LK8
M0S
M1P
M2P
M7P
PIMPY
PQEST
PQQKQ
PQUKI
PRINS
Q9U
5PM
DOI 10.1038/srep10014
DatabaseName SpringerOpen
Medline
MEDLINE
MEDLINE (Ovid)
MEDLINE
MEDLINE
PubMed
CrossRef
ProQuest Central (Corporate)
Health & Medical Collection
ProQuest Central (purchase pre-March 2016)
Biology Database (Alumni Edition)
Medical Database (Alumni Edition)
Science Database (Alumni Edition)
ProQuest SciTech Collection
ProQuest Natural Science Collection
Hospital Premium Collection
Hospital Premium Collection (Alumni Edition)
ProQuest Central (Alumni) (purchase pre-March 2016)
ProQuest Central (Alumni)
ProQuest Central
ProQuest Central Essentials
Biological Science Collection
ProQuest Central
Natural Science Collection
ProQuest One Community College
ProQuest Central Korea
Health Research Premium Collection
Health Research Premium Collection (Alumni)
ProQuest Central Student
SciTech Premium Collection
ProQuest Health & Medical Complete (Alumni)
ProQuest Biological Science Collection
Health & Medical Collection (Alumni Edition)
Medical Database
Science Database
Biological Science Database
Publicly Available Content Database
ProQuest One Academic Eastern Edition (DO NOT USE)
ProQuest One Academic
ProQuest One Academic UKI Edition
ProQuest Central China
ProQuest Central Basic
PubMed Central (Full Participant titles)
DatabaseTitle MEDLINE
Medline Complete
MEDLINE with Full Text
PubMed
MEDLINE (Ovid)
CrossRef
Publicly Available Content Database
ProQuest Central Student
ProQuest Central Essentials
ProQuest Health & Medical Complete (Alumni)
ProQuest Central (Alumni Edition)
SciTech Premium Collection
ProQuest One Community College
ProQuest Natural Science Collection
ProQuest Central China
ProQuest Biology Journals (Alumni Edition)
ProQuest Central
Health Research Premium Collection
Health and Medicine Complete (Alumni Edition)
Natural Science Collection
ProQuest Central Korea
Biological Science Collection
ProQuest Medical Library (Alumni)
ProQuest Science Journals (Alumni Edition)
ProQuest Biological Science Collection
ProQuest Central Basic
ProQuest Science Journals
ProQuest One Academic Eastern Edition
ProQuest Hospital Collection
Health Research Premium Collection (Alumni)
Biological Science Database
ProQuest SciTech Collection
ProQuest Hospital Collection (Alumni)
ProQuest Health & Medical Complete
ProQuest Medical Library
ProQuest One Academic UKI Edition
ProQuest One Academic
ProQuest Central (Alumni)
DatabaseTitleList

Publicly Available Content Database
MEDLINE
CrossRef
Database_xml – sequence: 1
  dbid: C6C
  name: SpringerOpen
  url: http://www.springeropen.com/
  sourceTypes: Publisher
– sequence: 2
  dbid: NPM
  name: PubMed
  url: https://proxy.k.utb.cz/login?url=http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed
  sourceTypes: Index Database
– sequence: 3
  dbid: EIF
  name: MEDLINE
  url: https://proxy.k.utb.cz/login?url=https://www.webofscience.com/wos/medline/basic-search
  sourceTypes: Index Database
– sequence: 4
  dbid: BENPR
  name: ProQuest Central
  url: https://www.proquest.com/central
  sourceTypes: Aggregation Database
DeliveryMethod fulltext_linktorsrc
Discipline Biology
EISSN 2045-2322
ExternalDocumentID 10_1038_srep10014
25950719
Genre Research Support, Non-U.S. Gov't
Journal Article
GroupedDBID 0R~
3V.
4.4
53G
5VS
7X7
88A
88E
88I
8FE
8FH
8FI
8FJ
AAFWJ
AAJSJ
AAKDD
ABDBF
ABUWG
ACGFS
ACSMW
ADBBV
ADRAZ
AENEX
AFKRA
AJTQC
ALIPV
ALMA_UNASSIGNED_HOLDINGS
AOIJS
AZQEC
BAWUL
BBNVY
BCNDV
BENPR
BHPHI
BPHCQ
BVXVI
C6C
CCPQU
DIK
DWQXO
EBD
EBLON
EBS
EJD
ESX
FYUFA
GNUQQ
GROUPED_DOAJ
GX1
HCIFZ
HH5
HMCUK
HYE
KQ8
LK8
M0L
M1P
M2P
M48
M7P
M~E
NAO
OK1
PIMPY
PQQKQ
PROAC
PSQYO
RNT
RNTTT
RPM
SNYQT
UKHRP
CGR
CUY
CVF
ECM
EIF
NPM
AAYXX
AFPKN
CITATION
7XB
8FK
K9.
PQEST
PQUKI
PRINS
Q9U
5PM
ID FETCH-LOGICAL-c438t-65c64da80548034c35ad26f91e1641ccf6df35dde858f3deb87718e3754786433
IEDL.DBID RPM
ISSN 2045-2322
IngestDate Tue Sep 17 21:27:39 EDT 2024
Thu Oct 10 15:58:48 EDT 2024
Fri Aug 23 00:33:59 EDT 2024
Tue Oct 15 23:53:24 EDT 2024
Fri Oct 11 20:46:59 EDT 2024
IsDoiOpenAccess true
IsOpenAccess true
IsPeerReviewed true
IsScholarly true
Issue 1
Language English
License This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0
LinkModel DirectLink
MergedId FETCHMERGED-LOGICAL-c438t-65c64da80548034c35ad26f91e1641ccf6df35dde858f3deb87718e3754786433
Notes These authors contributed equally to this work.
OpenAccessLink https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4423442/
PMID 25950719
PQID 1899447504
PQPubID 2041939
ParticipantIDs pubmedcentral_primary_oai_pubmedcentral_nih_gov_4423442
proquest_journals_1899447504
crossref_primary_10_1038_srep10014
pubmed_primary_25950719
springer_journals_10_1038_srep10014
PublicationCentury 2000
PublicationDate 2015-05-07
PublicationDateYYYYMMDD 2015-05-07
PublicationDate_xml – month: 05
  year: 2015
  text: 2015-05-07
  day: 07
PublicationDecade 2010
PublicationPlace London
PublicationPlace_xml – name: London
– name: England
PublicationTitle Scientific reports
PublicationTitleAbbrev Sci Rep
PublicationTitleAlternate Sci Rep
PublicationYear 2015
Publisher Nature Publishing Group UK
Nature Publishing Group
Publisher_xml – name: Nature Publishing Group UK
– name: Nature Publishing Group
References Hovatta (CR38) 2003; 18
Ginis (CR8) 2004; 269
Chen, Dong, Jiang, Lai, Guo (CR12) 2013; 22
Catalina (CR42) 2008; 7
Nichols, Smith (CR26) 2009; 4
Xu, Zhang, Huang (CR33) 2009; 15
Theunissen (CR31) 2014; 15
Johkura (CR19) 2004; 205
Richards (CR41) 2003; 21
Heins (CR15) 2004; 22
Yu (CR22) 2006; 318
Baharvand, Matthaei (CR18) 2003; 7
Miki, Lehmann, Cai, Stolz, Strom (CR13) 2005; 23
Lee (CR40) 2005; 72
Martin (CR7) 1981; 78
Xu (CR27) 2008; 3
Evans, Kaufman (CR6) 1981; 292
Tesar (CR24) 2007; 448
Dansranjavin (CR36) 2009; 8
Hyslop, Armstrong, Stojkovic, Lako (CR2) 2005; 7
Inzunza (CR39) 2005; 23
Sathananthan, Trounson (CR20) 2005; 110
Lai (CR9) 2009; 11
Reubinoff, Pera, Fong, Trounson, Bongso (CR14) 2000; 18
Lai (CR10) 2010; 12
Guhr, Kurtz, Friedgen, Loser (CR23) 2006; 24
Gafni (CR30) 2013; 504
Lai, Chen, Wang, Jiang, Wei (CR11) 2012; 14
Kee, Angeles, Flores, Nguyen, Reijo Pera (CR29) 2009; 462
Loh. (CR35) 2006; 38
Qiu, Ma, Wang, Peng, Huang (CR34) 2010; 38
Hanna (CR4) 2010; 107
Brons (CR25) 2007; 448
Gu (CR5) 2012; 3
Cheng (CR37) 2003; 21
Koyanagi-Katsuta, Akimitsu, Arimitsu, Hatano, Sekimizu (CR32) 2000; 32
Mateizel, Geens, Van de Velde, Sermon (CR16) 2012; 873
Mirhaghani, Warton (CR21) 1996; 82
Thomson (CR1) 1998; 282
Martin, Muotri, Gage, Varki (CR3) 2005; 11
Sathananthan, Pera, Trounson (CR17) 2002; 4
Silva, Rowntree, Mekhoubad, Lee (CR28) 2008; 105
19966271 - Nucleic Acids Res. 2010 Mar;38(4):1240-8
22384927 - Cell Reprogram. 2012 Apr;14(2):164-70
15317691 - Biol Reprod. 2005 Jan;72(1):42-9
15790775 - Stem Cells. 2005 Apr;23(4):544-9
19147696 - RNA. 2009 Mar;15(3):357-61
20442331 - Proc Natl Acad Sci U S A. 2010 May 18;107(20):9222-7
16778154 - Stem Cells. 2006 Oct;24(10):2187-91
12470354 - Reprod Biomed Online. 2002 Jan-Feb;4(1):56-61
22528350 - Methods Mol Biol. 2012;873:81-112
20698772 - Cell Reprogram. 2010 Jun;12(3):305-14
9804556 - Science. 1998 Nov 6;282(5391):1145-7
19865085 - Nature. 2009 Nov 12;462(7270):222-5
22985337 - Stem Cells Dev. 2013 Jan 15;22(2):320-9
7242681 - Nature. 1981 Jul 9;292(5819):154-6
18339803 - Proc Natl Acad Sci U S A. 2008 Mar 25;105(12):4820-5
12634409 - Stem Cells. 2003;21(2):131-42
16518401 - Nat Genet. 2006 Apr;38(4):431-40
12832363 - Hum Reprod. 2003 Jul;18(7):1404-9
15153613 - Stem Cells. 2004;22(3):367-76
19497275 - Cell Stem Cell. 2009 Jun 5;4(6):487-92
15685172 - Nat Med. 2005 Feb;11(2):228-32
25090446 - Cell Stem Cell. 2014 Oct 2;15(4):471-87
19508128 - Cloning Stem Cells. 2009 Jun;11(2):331-40
14653895 - Reprod Biomed Online. 2003 Oct;7(3):330-5
16081662 - Stem Cells. 2005 Nov-Dec;23(10):1549-59
22271597 - Protein Cell. 2012 Jan;3(1):71-9
15110706 - Dev Biol. 2004 May 15;269(2):360-80
15447684 - J Anat. 2004 Oct;205(4):247-55
18834512 - Mol Cancer. 2008;7:76
18682241 - Cell Stem Cell. 2008 Aug 7;3(2):196-206
16101033 - Ital J Anat Embryol. 2005;110(2 Suppl 1):151-7
17597762 - Nature. 2007 Jul 12;448(7150):191-5
10748519 - Nat Biotechnol. 2000 Apr;18(4):399-404
12968109 - Stem Cells. 2003;21(5):546-56
16171533 - Expert Rev Mol Med. 2005 Sep 19;7(19):1-21
8825444 - Parasitol Res. 1996;82(1):43-7
6950406 - Proc Natl Acad Sci U S A. 1981 Dec;78(12):7634-8
24172903 - Nature. 2013 Dec 12;504(7479):282-6
10798319 - Tissue Cell. 2000 Feb;32(1):66-70
18029452 - Science. 2007 Dec 21;318(5858):1917-20
19221495 - Cell Cycle. 2009 Mar 15;8(6):916-24
17597760 - Nature. 2007 Jul 12;448(7150):196-9
P Catalina (BFsrep10014_CR42) 2008; 7
MJ Evans (BFsrep10014_CR6) 1981; 292
O Hovatta (BFsrep10014_CR38) 2003; 18
JB Lee (BFsrep10014_CR40) 2005; 72
R Koyanagi-Katsuta (BFsrep10014_CR32) 2000; 32
J Yu (BFsrep10014_CR22) 2006; 318
T Dansranjavin (BFsrep10014_CR36) 2009; 8
K Kee (BFsrep10014_CR29) 2009; 462
L Cheng (BFsrep10014_CR37) 2003; 21
GR Martin (BFsrep10014_CR7) 1981; 78
BE Reubinoff (BFsrep10014_CR14) 2000; 18
JA Thomson (BFsrep10014_CR1) 1998; 282
D Lai (BFsrep10014_CR11) 2012; 14
D Lai (BFsrep10014_CR10) 2010; 12
I Ginis (BFsrep10014_CR8) 2004; 269
T Miki (BFsrep10014_CR13) 2005; 23
RH Xu (BFsrep10014_CR27) 2008; 3
A Guhr (BFsrep10014_CR23) 2006; 24
TW Theunissen (BFsrep10014_CR31) 2014; 15
YH Loh. (BFsrep10014_CR35) 2006; 38
YF Chen (BFsrep10014_CR12) 2013; 22
IG Brons (BFsrep10014_CR25) 2007; 448
J Nichols (BFsrep10014_CR26) 2009; 4
A Mirhaghani (BFsrep10014_CR21) 1996; 82
N Heins (BFsrep10014_CR15) 2004; 22
MJ Martin (BFsrep10014_CR3) 2005; 11
B Xu (BFsrep10014_CR33) 2009; 15
H Baharvand (BFsrep10014_CR18) 2003; 7
D Lai (BFsrep10014_CR9) 2009; 11
H Sathananthan (BFsrep10014_CR17) 2002; 4
AH Sathananthan (BFsrep10014_CR20) 2005; 110
O Gafni (BFsrep10014_CR30) 2013; 504
C Qiu (BFsrep10014_CR34) 2010; 38
I Mateizel (BFsrep10014_CR16) 2012; 873
K Johkura (BFsrep10014_CR19) 2004; 205
PJ Tesar (BFsrep10014_CR24) 2007; 448
J Inzunza (BFsrep10014_CR39) 2005; 23
M Richards (BFsrep10014_CR41) 2003; 21
LA Hyslop (BFsrep10014_CR2) 2005; 7
SS Silva (BFsrep10014_CR28) 2008; 105
Q Gu (BFsrep10014_CR5) 2012; 3
J Hanna (BFsrep10014_CR4) 2010; 107
References_xml – volume: 269
  start-page: 360
  year: 2004
  end-page: 380
  ident: CR8
  article-title: Differences between human and mouse embryonic stem cells
  publication-title: Dev. Biol.
  doi: 10.1016/j.ydbio.2003.12.034
  contributor:
    fullname: Ginis
– volume: 318
  start-page: 1917
  year: 2006
  end-page: 1920
  ident: CR22
  article-title: Induced pluripotent stem cell lines derived from human somatic cells
  publication-title: Science
  doi: 10.1126/science.1151526
  contributor:
    fullname: Yu
– volume: 3
  start-page: 196
  year: 2008
  end-page: 206
  ident: CR27
  article-title: NANOG is a direct target of TGFβ/Activin-mediated SMAD signaling in human ESCs
  publication-title: Cell Stem Cell
  doi: 10.1016/j.stem.2008.07.001
  contributor:
    fullname: Xu
– volume: 4
  start-page: 487
  year: 2009
  end-page: 492
  ident: CR26
  article-title: Naive and primed pluripotent states
  publication-title: Cell Stem Cell
  doi: 10.1016/j.stem.2009.05.015
  contributor:
    fullname: Smith
– volume: 32
  start-page: 66
  year: 2000
  end-page: 70
  ident: CR32
  article-title: Apoptosis of mouse embryonic stem cells induced by single cell suspension
  publication-title: Tissue Cell
  doi: 10.1054/tice.1999.0087
  contributor:
    fullname: Sekimizu
– volume: 23
  start-page: 544
  year: 2005
  end-page: 549
  ident: CR39
  article-title: Derivation of human embryonic stem cell lines in serum replacement medium using postnatal human fibroblasts as feeder cells
  publication-title: Stem Cells
  doi: 10.1634/stemcells.2004-0201
  contributor:
    fullname: Inzunza
– volume: 873
  start-page: 81
  year: 2012
  end-page: 112
  ident: CR16
  article-title: Establishment of hESC lines from the inner cell mass of blastocyst-stage embryos and single blastomeres of 4-cell stage embryos
  publication-title: Methods Mol. Biol.
  doi: 10.1007/978-1-61779-794-1_6
  contributor:
    fullname: Sermon
– volume: 82
  start-page: 43
  year: 1996
  end-page: 47
  ident: CR21
  article-title: An electron microscope study of the interaction between Trichomonas vaginalis and epithelial cells of the human amnion membrane
  publication-title: Parasitol Res.
  doi: 10.1007/s004360050066
  contributor:
    fullname: Warton
– volume: 448
  start-page: 196
  year: 2007
  end-page: 199
  ident: CR24
  article-title: New cell lines from mouse epiblast share defining features with human embryonic stem cells
  publication-title: Nature
  doi: 10.1038/nature05972
  contributor:
    fullname: Tesar
– volume: 18
  start-page: 1404
  year: 2003
  end-page: 1409
  ident: CR38
  article-title: A culture system using human foreskin fibroblasts as feeder cells allows production of human embryonic stem cells
  publication-title: Hum. Reprod.
  doi: 10.1093/humrep/deg290
  contributor:
    fullname: Hovatta
– volume: 24
  start-page: 2187
  year: 2006
  end-page: 2191
  ident: CR23
  article-title: Current state of human embryonic stem cell research: an overview of cell lines and their usage in experimental work
  publication-title: Stem Cells.
  doi: 10.1634/stemcells.2006-0053
  contributor:
    fullname: Loser
– volume: 22
  start-page: 320
  year: 2013
  end-page: 329
  ident: CR12
  article-title: Mouse primed embryonic stem cells could be maintained and reprogrammed on human amnion epithelial cells
  publication-title: Stem Cells Dev.
  doi: 10.1089/scd.2012.0325
  contributor:
    fullname: Guo
– volume: 23
  start-page: 1549
  year: 2005
  end-page: 1559
  ident: CR13
  article-title: Stem cell characteristics of amniotic epithelial cells
  publication-title: Stem Cells
  doi: 10.1634/stemcells.2004-0357
  contributor:
    fullname: Strom
– volume: 14
  start-page: 164
  year: 2012
  end-page: 170
  ident: CR11
  article-title: LKB1 controls the pluripotent state of human embryonic stem cells
  publication-title: Cell Reprogram
  doi: 10.1089/cell.2011.0068
  contributor:
    fullname: Wei
– volume: 504
  start-page: 282
  year: 2013
  end-page: 286
  ident: CR30
  article-title: Derivation of novel human ground state naive pluripotent stem cells
  publication-title: Nature
  doi: 10.1038/nature12745
  contributor:
    fullname: Gafni
– volume: 15
  start-page: 471
  year: 2014
  end-page: 487
  ident: CR31
  article-title: Systematic Identification of Culture Conditions for Induction and Maintenance of Naive Human Pluripotency
  publication-title: Cell Stem Cell
  doi: 10.1016/j.stem.2014.07.002
  contributor:
    fullname: Theunissen
– volume: 78
  start-page: 7634
  year: 1981
  end-page: 7638
  ident: CR7
  article-title: Isolation of a pluripotent cell line from early mouse embryos cultured in medium conditioned by teratocarcinoma stem cells
  publication-title: Proc. Natl. Acad. Sci. U S A
  doi: 10.1073/pnas.78.12.7634
  contributor:
    fullname: Martin
– volume: 7
  start-page: 76
  year: 2008
  end-page: 82
  ident: CR42
  article-title: Human ESCs predisposition to karyotypic instability: is a matter of culture adaptation or differential vulnerability among hESC lines due to inherent properties ? 
  publication-title: Mol. Cancer
  doi: 10.1186/1476-4598-7-76
  contributor:
    fullname: Catalina
– volume: 4
  start-page: 56
  year: 2002
  end-page: 61
  ident: CR17
  article-title: The fine structure of human embryonic stem cells
  publication-title: Reprod. Biomed Online
  doi: 10.1016/S1472-6483(10)61916-5
  contributor:
    fullname: Trounson
– volume: 11
  start-page: 331
  year: 2009
  end-page: 340
  ident: CR9
  article-title: Use of human amnion epithelial cells as a feeder layer to support undifferentiated growth of mouse embryonic stem cells
  publication-title: Cloning Stem Cells
  doi: 10.1089/clo.2008.0047
  contributor:
    fullname: Lai
– volume: 105
  start-page: 4820
  year: 2008
  end-page: 4825
  ident: CR28
  article-title: X-chromosome inactivation and epigenetic fluidity in human embryonic stem cells
  publication-title: Proc. Natl. Acad. Sci. USA
  doi: 10.1073/pnas.0712136105
  contributor:
    fullname: Lee
– volume: 22
  start-page: 367
  year: 2004
  end-page: 376
  ident: CR15
  article-title: Derivation, characterization and differentiation of human embryonic stem cells
  publication-title: Stem Cells
  doi: 10.1634/stemcells.22-3-367
  contributor:
    fullname: Heins
– volume: 282
  start-page: 1145
  year: 1998
  end-page: 1147
  ident: CR1
  article-title: Embryonic stem cell lines derived from human blastocysts
  publication-title: Science
  doi: 10.1126/science.282.5391.1145
  contributor:
    fullname: Thomson
– volume: 3
  start-page: 71
  year: 2012
  end-page: 79
  ident: CR5
  article-title: Rapid conversion of human ESCs into mouse ESC-like pluripotent state by optimizing culture conditions
  publication-title: Protein Cel.
  doi: 10.1007/s13238-012-2007-8
  contributor:
    fullname: Gu
– volume: 448
  start-page: 191
  year: 2007
  end-page: 195
  ident: CR25
  article-title: Derivation of pluripotent epiblast stem cells from mammalian embryos
  publication-title: Nature
  doi: 10.1038/nature05950
  contributor:
    fullname: Brons
– volume: 7
  start-page: 330
  year: 2003
  end-page: 335
  ident: CR18
  article-title: The ultrastructure of mouse embryonic stem cells
  publication-title: Reprod Biomed Online
  doi: 10.1016/S1472-6483(10)61873-1
  contributor:
    fullname: Matthaei
– volume: 38
  start-page: 431
  year: 2006
  end-page: 440
  ident: CR35
  article-title: The Oct4 and Nanog transcription network regulates pluripotency in mouse embryonic stem cells
  publication-title: Nat. Genet.
  doi: 10.1038/ng1760
  contributor:
    fullname: Loh.
– volume: 292
  start-page: 154
  year: 1981
  end-page: 156
  ident: CR6
  article-title: Establishment in culture of pluripotential cells from mouse embryos
  publication-title: Nature
  doi: 10.1038/292154a0
  contributor:
    fullname: Kaufman
– volume: 72
  start-page: 42
  year: 2005
  end-page: 49
  ident: CR40
  article-title: Establishment and maintenance of human embryonic stem cell lines on human feeder cells derived from uterine endometrium under serumfree condition
  publication-title: Biol. Reprod.
  doi: 10.1095/biolreprod.104.033480
  contributor:
    fullname: Lee
– volume: 18
  start-page: 399
  year: 2000
  end-page: 404
  ident: CR14
  article-title: Embryonic stem cell lines from human blastocysts: somatic differentiation in vitro
  publication-title: Nat Biotechnol.
  doi: 10.1038/74447
  contributor:
    fullname: Bongso
– volume: 11
  start-page: 228
  year: 2005
  end-page: 232
  ident: CR3
  article-title: Human embryonic stem cells express an immunogenic nonhuman sialic acid
  publication-title: Nat. Med.
  doi: 10.1038/nm1181
  contributor:
    fullname: Varki
– volume: 107
  start-page: 9222
  year: 2010
  end-page: 9227
  ident: CR4
  article-title: Human embryonic stem cells with biological and epigenetic characteristics similar to those of mouse ESCs
  publication-title: Proc. Natl. Acad. Sci. USA
  doi: 10.1073/pnas.1004584107
  contributor:
    fullname: Hanna
– volume: 462
  start-page: 222
  year: 2009
  end-page: 225
  ident: CR29
  article-title: Human DAZL, DAZ and BOULE genes modulate primordial germ-cell and haploid gamete formation
  publication-title: Nature
  doi: 10.1038/nature08562
  contributor:
    fullname: Reijo Pera
– volume: 12
  start-page: 305
  year: 2010
  end-page: 314
  ident: CR10
  article-title: Optimization of culture conditions to support undifferentiated growth of human embryonic stem cells
  publication-title: Cell Reprogram
  doi: 10.1089/cell.2009.0106
  contributor:
    fullname: Lai
– volume: 8
  start-page: 916
  year: 2009
  end-page: 824
  ident: CR36
  article-title: The role of promoter CpG methylation in the epigenetic control of stem cell related genes during differentiation
  publication-title: Cell Cycle
  doi: 10.4161/cc.8.6.7934
  contributor:
    fullname: Dansranjavin
– volume: 38
  start-page: 1240
  year: 2010
  end-page: 1248
  ident: CR34
  article-title: Lin28-mediated post-transcriptional regulation of Oct4 expression in human embryonic stem cells
  publication-title: Nucleic Acids Res.
  doi: 10.1093/nar/gkp1071
  contributor:
    fullname: Huang
– volume: 21
  start-page: 131
  year: 2003
  end-page: 142
  ident: CR37
  article-title: Human adult marrow cells support prolonged expansion of human embryonic stem cells in culture
  publication-title: Stem Cells
  doi: 10.1634/stemcells.21-2-131
  contributor:
    fullname: Cheng
– volume: 7
  start-page: 1
  year: 2005
  end-page: 21
  ident: CR2
  article-title: Human embryonic stem cells: biology and clinical implications
  publication-title: Expert Rev. Mol. Med.
  doi: 10.1017/S1462399405009804
  contributor:
    fullname: Lako
– volume: 205
  start-page: 247
  year: 2004
  end-page: 255
  ident: CR19
  article-title: Cytochemical and ultrastructural characterization of growing colonies of human embryonic stem cells
  publication-title: J. Anat.
  doi: 10.1111/j.0021-8782.2004.00336.x
  contributor:
    fullname: Johkura
– volume: 110
  start-page: 151
  year: 2005
  end-page: 157
  ident: CR20
  article-title: Human embryonic stem cells and their spontaneous differentiation
  publication-title: Ital. J. Anat. Embryol.
  contributor:
    fullname: Trounson
– volume: 15
  start-page: 357
  year: 2009
  end-page: 361
  ident: CR33
  article-title: Lin28 modulates cell growth and associates with a subset of cell cycle regulator mRNAs in mouse embryonic stem cells
  publication-title: . RNA
  doi: 10.1261/rna.1368009
  contributor:
    fullname: Huang
– volume: 21
  start-page: 546
  year: 2003
  end-page: 556
  ident: CR41
  article-title: A comparative evaluation of various human feeders for prolonged undifferentiated growth of human embryonic stem cells
  publication-title: Stem Cells
  doi: 10.1634/stemcells.21-5-546
  contributor:
    fullname: Richards
– volume: 7
  start-page: 330
  year: 2003
  ident: BFsrep10014_CR18
  publication-title: Reprod Biomed Online
  doi: 10.1016/S1472-6483(10)61873-1
  contributor:
    fullname: H Baharvand
– volume: 32
  start-page: 66
  year: 2000
  ident: BFsrep10014_CR32
  publication-title: Tissue Cell
  doi: 10.1054/tice.1999.0087
  contributor:
    fullname: R Koyanagi-Katsuta
– volume: 269
  start-page: 360
  year: 2004
  ident: BFsrep10014_CR8
  publication-title: Dev. Biol.
  doi: 10.1016/j.ydbio.2003.12.034
  contributor:
    fullname: I Ginis
– volume: 24
  start-page: 2187
  year: 2006
  ident: BFsrep10014_CR23
  publication-title: Stem Cells.
  doi: 10.1634/stemcells.2006-0053
  contributor:
    fullname: A Guhr
– volume: 38
  start-page: 1240
  year: 2010
  ident: BFsrep10014_CR34
  publication-title: Nucleic Acids Res.
  doi: 10.1093/nar/gkp1071
  contributor:
    fullname: C Qiu
– volume: 105
  start-page: 4820
  year: 2008
  ident: BFsrep10014_CR28
  publication-title: Proc. Natl. Acad. Sci. USA
  doi: 10.1073/pnas.0712136105
  contributor:
    fullname: SS Silva
– volume: 504
  start-page: 282
  year: 2013
  ident: BFsrep10014_CR30
  publication-title: Nature
  doi: 10.1038/nature12745
  contributor:
    fullname: O Gafni
– volume: 3
  start-page: 71
  year: 2012
  ident: BFsrep10014_CR5
  publication-title: Protein Cel.
  doi: 10.1007/s13238-012-2007-8
  contributor:
    fullname: Q Gu
– volume: 12
  start-page: 305
  year: 2010
  ident: BFsrep10014_CR10
  publication-title: Cell Reprogram
  doi: 10.1089/cell.2009.0106
  contributor:
    fullname: D Lai
– volume: 205
  start-page: 247
  year: 2004
  ident: BFsrep10014_CR19
  publication-title: J. Anat.
  doi: 10.1111/j.0021-8782.2004.00336.x
  contributor:
    fullname: K Johkura
– volume: 22
  start-page: 320
  year: 2013
  ident: BFsrep10014_CR12
  publication-title: Stem Cells Dev.
  doi: 10.1089/scd.2012.0325
  contributor:
    fullname: YF Chen
– volume: 448
  start-page: 191
  year: 2007
  ident: BFsrep10014_CR25
  publication-title: Nature
  doi: 10.1038/nature05950
  contributor:
    fullname: IG Brons
– volume: 448
  start-page: 196
  year: 2007
  ident: BFsrep10014_CR24
  publication-title: Nature
  doi: 10.1038/nature05972
  contributor:
    fullname: PJ Tesar
– volume: 23
  start-page: 1549
  year: 2005
  ident: BFsrep10014_CR13
  publication-title: Stem Cells
  doi: 10.1634/stemcells.2004-0357
  contributor:
    fullname: T Miki
– volume: 3
  start-page: 196
  year: 2008
  ident: BFsrep10014_CR27
  publication-title: Cell Stem Cell
  doi: 10.1016/j.stem.2008.07.001
  contributor:
    fullname: RH Xu
– volume: 4
  start-page: 487
  year: 2009
  ident: BFsrep10014_CR26
  publication-title: Cell Stem Cell
  doi: 10.1016/j.stem.2009.05.015
  contributor:
    fullname: J Nichols
– volume: 21
  start-page: 131
  year: 2003
  ident: BFsrep10014_CR37
  publication-title: Stem Cells
  doi: 10.1634/stemcells.21-2-131
  contributor:
    fullname: L Cheng
– volume: 18
  start-page: 1404
  year: 2003
  ident: BFsrep10014_CR38
  publication-title: Hum. Reprod.
  doi: 10.1093/humrep/deg290
  contributor:
    fullname: O Hovatta
– volume: 282
  start-page: 1145
  year: 1998
  ident: BFsrep10014_CR1
  publication-title: Science
  doi: 10.1126/science.282.5391.1145
  contributor:
    fullname: JA Thomson
– volume: 873
  start-page: 81
  year: 2012
  ident: BFsrep10014_CR16
  publication-title: Methods Mol. Biol.
  doi: 10.1007/978-1-61779-794-1_6
  contributor:
    fullname: I Mateizel
– volume: 11
  start-page: 228
  year: 2005
  ident: BFsrep10014_CR3
  publication-title: Nat. Med.
  doi: 10.1038/nm1181
  contributor:
    fullname: MJ Martin
– volume: 8
  start-page: 916
  year: 2009
  ident: BFsrep10014_CR36
  publication-title: Cell Cycle
  doi: 10.4161/cc.8.6.7934
  contributor:
    fullname: T Dansranjavin
– volume: 23
  start-page: 544
  year: 2005
  ident: BFsrep10014_CR39
  publication-title: Stem Cells
  doi: 10.1634/stemcells.2004-0201
  contributor:
    fullname: J Inzunza
– volume: 82
  start-page: 43
  year: 1996
  ident: BFsrep10014_CR21
  publication-title: Parasitol Res.
  doi: 10.1007/s004360050066
  contributor:
    fullname: A Mirhaghani
– volume: 11
  start-page: 331
  year: 2009
  ident: BFsrep10014_CR9
  publication-title: Cloning Stem Cells
  doi: 10.1089/clo.2008.0047
  contributor:
    fullname: D Lai
– volume: 462
  start-page: 222
  year: 2009
  ident: BFsrep10014_CR29
  publication-title: Nature
  doi: 10.1038/nature08562
  contributor:
    fullname: K Kee
– volume: 318
  start-page: 1917
  year: 2006
  ident: BFsrep10014_CR22
  publication-title: Science
  doi: 10.1126/science.1151526
  contributor:
    fullname: J Yu
– volume: 7
  start-page: 1
  year: 2005
  ident: BFsrep10014_CR2
  publication-title: Expert Rev. Mol. Med.
  doi: 10.1017/S1462399405009804
  contributor:
    fullname: LA Hyslop
– volume: 4
  start-page: 56
  year: 2002
  ident: BFsrep10014_CR17
  publication-title: Reprod. Biomed Online
  doi: 10.1016/S1472-6483(10)61916-5
  contributor:
    fullname: H Sathananthan
– volume: 78
  start-page: 7634
  year: 1981
  ident: BFsrep10014_CR7
  publication-title: Proc. Natl. Acad. Sci. U S A
  doi: 10.1073/pnas.78.12.7634
  contributor:
    fullname: GR Martin
– volume: 107
  start-page: 9222
  year: 2010
  ident: BFsrep10014_CR4
  publication-title: Proc. Natl. Acad. Sci. USA
  doi: 10.1073/pnas.1004584107
  contributor:
    fullname: J Hanna
– volume: 15
  start-page: 357
  year: 2009
  ident: BFsrep10014_CR33
  publication-title: . RNA
  doi: 10.1261/rna.1368009
  contributor:
    fullname: B Xu
– volume: 21
  start-page: 546
  year: 2003
  ident: BFsrep10014_CR41
  publication-title: Stem Cells
  doi: 10.1634/stemcells.21-5-546
  contributor:
    fullname: M Richards
– volume: 15
  start-page: 471
  year: 2014
  ident: BFsrep10014_CR31
  publication-title: Cell Stem Cell
  doi: 10.1016/j.stem.2014.07.002
  contributor:
    fullname: TW Theunissen
– volume: 38
  start-page: 431
  year: 2006
  ident: BFsrep10014_CR35
  publication-title: Nat. Genet.
  doi: 10.1038/ng1760
  contributor:
    fullname: YH Loh.
– volume: 7
  start-page: 76
  year: 2008
  ident: BFsrep10014_CR42
  publication-title: Mol. Cancer
  doi: 10.1186/1476-4598-7-76
  contributor:
    fullname: P Catalina
– volume: 110
  start-page: 151
  year: 2005
  ident: BFsrep10014_CR20
  publication-title: Ital. J. Anat. Embryol.
  contributor:
    fullname: AH Sathananthan
– volume: 22
  start-page: 367
  year: 2004
  ident: BFsrep10014_CR15
  publication-title: Stem Cells
  doi: 10.1634/stemcells.22-3-367
  contributor:
    fullname: N Heins
– volume: 72
  start-page: 42
  year: 2005
  ident: BFsrep10014_CR40
  publication-title: Biol. Reprod.
  doi: 10.1095/biolreprod.104.033480
  contributor:
    fullname: JB Lee
– volume: 292
  start-page: 154
  year: 1981
  ident: BFsrep10014_CR6
  publication-title: Nature
  doi: 10.1038/292154a0
  contributor:
    fullname: MJ Evans
– volume: 14
  start-page: 164
  year: 2012
  ident: BFsrep10014_CR11
  publication-title: Cell Reprogram
  doi: 10.1089/cell.2011.0068
  contributor:
    fullname: D Lai
– volume: 18
  start-page: 399
  year: 2000
  ident: BFsrep10014_CR14
  publication-title: Nat Biotechnol.
  doi: 10.1038/74447
  contributor:
    fullname: BE Reubinoff
SSID ssj0000529419
Score 2.2941322
Snippet Culture conditions that support the growth of undifferentiated human embryonic stem cells (hESCs) have already been established using primary human amnion...
Abstract Culture conditions that support the growth of undifferentiated human embryonic stem cells (hESCs) have already been established using primary human...
SourceID pubmedcentral
proquest
crossref
pubmed
springer
SourceType Open Access Repository
Aggregation Database
Index Database
Publisher
StartPage 10014
SubjectTerms 13
13/1
13/100
13/106
13/51
14
14/105
14/28
45
631/532
631/532/2117
Amnion
Amnion - cytology
Biomarkers
Blastocyst - cytology
Cell culture
Cell Culture Techniques
Cell Differentiation
Cell Line
Cell lines
Chromosome Banding
Cytoplasm
Electron microscopy
Embryo cells
Embryo fibroblasts
Embryonic Stem Cells - cytology
Embryonic Stem Cells - metabolism
Embryonic Stem Cells - ultrastructure
Embryos
Epithelial cells
Feeder Cells
Gene Expression Profiling
Gene Expression Regulation, Developmental
Humanities and Social Sciences
Humans
In vitro fertilization
multidisciplinary
Pluripotency
Pluripotent Stem Cells - cytology
Pluripotent Stem Cells - metabolism
Pluripotent Stem Cells - ultrastructure
Science
Stem cell transplantation
Stem cells
Teratoma
Transmission electron microscopy
SummonAdditionalLinks – databaseName: Health & Medical Collection
  dbid: 7X7
  link: http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwfV1LS8NAEB60IngR30arLOo1NJvdJJuTiFqKoCcLPRmyj6Bg09jWQ_-9s3lpW_CQ0w5JmJndmZ3Z_T6Am4AqIX1pXE_HzOU0zdxYqQwnXsQNjbTWJcfS80s4GPKnUTCqC26z-lhlsyaWC7WeKFsj71HcGFhwOo_fFl-uZY2y3dWaQmMTtqiPzov-HI2itsZiu1icxg2gEBM9DDuFRR3iy2FoLbdcPyK50ictw09_D3brvJHcVYbehw2TH8B2xSS5OIS3B_SkqrpK0lwT1eIwV9csySQjJR0fMWM5XVg8XGIhnIkt3M8IClSj6Ti30qawVzU-0TcrgSMY9h9f7wduTZ3gKs7E3A0DFXKdCs_CuTGuWJBqP8xianB7RNEYoc5YgEubCETGtJFoGyqM5cONBCYp7Bg6-SQ3p0BkjEqIhNKaezyjQkqTBjLUGPdZSpXvwFWjyaSoEDKSsrPNRNKq24Fuo-OkniSz5NekDpxU6m7fgLsym6nGDkRLhmgFLCz28kj-8V7CY3PMEPFx4Lox2Z9Prv7Y2f8_dg47mCEF5QnHqAud-fTbXGAWMpeXpav9AFBm4Nc
  priority: 102
  providerName: ProQuest
– databaseName: Scholars Portal Open Access Journals
  dbid: M48
  link: http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwdV3LSsQwFL2MiuBGfFtfBHVbnTRJmy5ERB1E0JUDrizNoyhoHccRnL_3pmnLjKOLrnL74J6kOXmdA3AsqJYqUjbsmpSFnOZFmGpdYMNLuKWJMabyWLq7j2_6_PZRPHag8disE_j559DO-Un1h68n3x_jc2zwZ_7IuDzFvmTgpIT4HCxETo_L7eCrWb6X-I5STtNGV2jyDqcFLFLHidLpjmmGbc5umvy1clp1SL0VWK6ZJLnw0K9Cx5ZrsOi9Jcfr8HSFdcvPt5K8NES3ysz-4CV5L0hl0EfsmxqOnUIucaLOxE3lfxIM8KX5W-mi7cAd3njF2uoDNqDfu364vAlrM4VQcyZHYSx0zE0uu07gjXHNRG6iuEipxQETRXhiUzCBPzspZMGMVYgWldY55CYSaQvbhPnyvbTbQFSKSUikNoZ3eUGlUjYXKjbIBFhOdRTAYZPJbOA1M7JqrZvJrM18AHtNjrMG9Yzi6M8pEHaxeMunu31Cg1MAyRQQbYATyp4uKV-eK8FsjpwRrwCOGsgmXvn7w3b-ffMuLCFdEtV2x2QP5kfDL7uPlGSkDqoK9wP-EOK0
  priority: 102
  providerName: Scholars Portal
– databaseName: SpringerOpen
  dbid: C6C
  link: http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwjV07T8MwED6VIiQWxJtAQeaxRsS1nTgjKlQVEkxU6kQUPyKQaFq1Zei_55w0UdMuDJl8eeiznfvOZ38H8CColqqrrB-YmPmcppkfa53hxIu4pZExpqix9PYeDob8dSRGLbitzsI08vdMPqKfmDqZIL4Du871uuoMvbBXL6O4RBWncaUZtH5H09Ns0cftXZAbqdDCw_QP4WBFDclT2ZdH0LL5MeyVxSKXJ_D5jIOlXEAlGP4TXUstlycpySQjRcU9YsdqtnSSt8SpNBO3Nj8naFC2puPcWdupO43xg8OvNDiFYf_lozfwV9URfM2ZXPih0CE3qQycYhvjmonUdMMsphYjIIp4hyZjAv9eUsiMGasQfiqtK3kbSeQh7Aza-SS3F0BUjCBEUhvDA55RqZRNhQoNunaWUt314K5CMpmWIhhJkbxmMqnh9qBTYZys5sE8oRjOOUnBAJvPS7jrJ2Dg5cho7EHU6IjawClfN1vy769CAZsjCcTLg_uqy9Zeuflhl_-yuoJ95EKi2MsYdaC9mP3aa-QbC3VTjLg_88vWzQ
  priority: 102
  providerName: Springer Nature
Title Derivation and characterization of human embryonic stem cells on human amnion epithelial cells
URI https://link.springer.com/article/10.1038/srep10014
https://www.ncbi.nlm.nih.gov/pubmed/25950719
https://www.proquest.com/docview/1899447504
https://pubmed.ncbi.nlm.nih.gov/PMC4423442
Volume 5
hasFullText 1
inHoldings 1
isFullTextHit
isPrint
link http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV3dS8MwED_mRPBF_HZ-jKC-1i1L0qaPOpUhbAxR2JOl-SgOXB06H_bfe0nW4fTNhxZKjjbcXZrfJZffAVwKqqXqKBu1TcoiTvMiSrUucOAl3NLEGONrLPUHce-ZP4zEqAaiOgvjk_a1Gl-Vb5OrcvzqcyunE92q8sRaw36XIwbAq7UGa-igP0L0QOjdSTlNKxYhJls410wd1ZCrwYNo3yGgdHUa-oMt_6ZI_ton9dPP_TZsLXAjuQ7924GaLXdhI1SSnO_Byy16UlhdJXlpiF7yMIdjluS9IL4cH7ET9TF3fLjEUTgTt3D_SVAgtOaT0knbqTuq8Ya-GQT24fn-7qnbixalEyLNmZxFsdAxN7lsOzo3xjUTuenERUothkcUjRGbggn8tUkhC2asQttQaV093EQiSGEHUC_fS3sERKWohERqY3ibF1QqZXOhYoPzPsup7jTgvNJkNg0MGZnf2WYyW2q-AaeVjrPFIPnMKMZ6jm-wjc2HQd3LN1R2akCyYoilgKPFXm1Bb_H02AvvaMBFZbIfn_zdseN_v_8ENhE8CZ_8mJxCffbxZc8QoMxUE91ylDRh_eZuMHzEp27cbfpgH-99LpveYb8B0iHu4g
link.rule.ids 230,315,730,783,787,867,888,12070,21402,24332,27938,27939,31733,33758,41134,42203,43324,43819,51590,53806,53808,74081,74638
linkProvider National Library of Medicine
linkToHtml http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwfV1LT8MwDLZgCMEF8aY8I-BasSxpm54QAqbxPIG0E1XzqEBi3WDjwL_H7gsGEoeeYqWRncSOnXwfwHHAjdId7fy2jYUveZr5sTEZLrxIOh5ZawuOpbv7sPcor_tBv0q4jatrlfWeWGzUdmgoR37C8WBA4HRteTp684k1iqqrFYXGLMwRDhdh50f9qMmxUBVL8rgGFBLqBN3OiFCH5LQb-hNb_r0i-atOWrif7jIsVXEjOysNvQIzLl-F-ZJJ8nMNni5wJpXZVZbmlpkGh7l8ZsmGGSvo-Jgb6PdPwsNlBOHMKHE_ZihQtqaDnKTdiJ5qvOLcLAXW4bF7-XDe8yvqBN9IoSZ-GJhQ2lS1Cc5NSCOC1HbCLOYOj0ccjRHaTAS4talAZcI6jbbhyhEfbqQwSBEb0MqHudsCpmNUQqSMtbItM660dmmgQ4t-X6TcdDw4rDWZjEqEjKSobAuVNOr2YLfWcVItknHybVIPNkt1Nz3gqYwi1diDaMoQjQDBYk-35C_PBTy2xAgRPw-OapP9-OXvgW3_P7ADWOg93N0mt1f3NzuwiNFSUNx2jHahNXn_cHsYkUz0fjHtvgALzePH
linkToPdf http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwfV1LT8MwDLZ4CMQF8aYwIAKu1ZYlbdITQsDEc-LApJ2omkcFEusGGwf-PU5fMCZx6ClWW9lO_CV2PgOcBlRL1VbWb5mI-ZwmqR9pneLEE9xSYYzJeyw9dMPrHr_tB_2y_mlcllVWa2K-UJuhdmfkTYobA0dO1-LNtCyLeLzsnI3efddBymVay3Ya87AoOAY69G3RF_V5i8tocRpV5EJMNjEEjRwDEZ8OSTM4c7Zc8k_ONA9FnTVYLTEkOS-Mvg5zNtuApaKr5NcmPF-iVxUnrSTJDNE1J3Nx5ZIMU5K35iN2oD6-HDcucXTOxB3ijwkKFKPJIHPSduSubbyhnxYCW9DrXD1dXPtlGwVfcyYnfhjokJtEthy1G-OaBYlph2lELW6VKBomNCkLcJmTgUyZsQrtRKV1vXGFRMDCtmEhG2Z2F4iKUAlCamN4i6dUKmWTQIUGMQBLqG57cFxpMh4VbBlxnuVmMq7V7UGj0nFcTphx_GNeD3YKdddvwB2aQ62RB2LKELWAo8ieHsleX3KqbI5oER8PTiqT_frk3x_b-__HjmAZPS6-v-ne7cMKAqcgL3wUDViYfHzaAwQnE3WYe903ZHPn_A
openUrl ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Derivation+and+characterization+of+human+embryonic+stem+cells+on+human+amnion+epithelial+cells&rft.jtitle=Scientific+reports&rft.au=Lai%2C+Dongmei&rft.au=Wang%2C+Yongwei&rft.au=Sun%2C+Jian&rft.au=Chen%2C+Yifei&rft.date=2015-05-07&rft.eissn=2045-2322&rft.volume=5&rft.spage=10014&rft_id=info:doi/10.1038%2Fsrep10014&rft_id=info%3Apmid%2F25950719&rft.externalDocID=25950719
thumbnail_l http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=2045-2322&client=summon
thumbnail_m http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=2045-2322&client=summon
thumbnail_s http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=2045-2322&client=summon