Pea seed extracts stimulate germination of the terrestrial orchid Ophrys apifera Huds. during a habitat restoration project

Novel methods are required to break the seed dormancy of temperate-zone orchids and aid the conservation of rare species. Zeatin is produced in increasing concentrations during the development of pea (Pisum sativum) seeds. We hypothesised that hot water extracts of pea seeds stimulate germination of...

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Published inPlant biosystems Vol. 149; no. 1; pp. 54 - 60
Main Authors Pierce, Simon, Guidi, Valentina, Ferrario, Andrea, Ceriani, Roberta M., Labra, Massimo, Vagge, Ilda, Cerabolini, Bruno E. L.
Format Journal Article
LanguageEnglish
Published Abingdon Taylor & Francis 02.01.2015
Taylor & Francis Ltd
Subjects
Asymbiotic germination
bee orchid
coconut milk
culture media
cytokinin
dry peas
Environmental restoration
germination
habitat conservation
Hormones
in vitro
mature plants
Ophrys
Orchidaceae
Pisum sativum
Rare species
seed dormancy
seed extracts
Seeds
zeatin
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Abstract Novel methods are required to break the seed dormancy of temperate-zone orchids and aid the conservation of rare species. Zeatin is produced in increasing concentrations during the development of pea (Pisum sativum) seeds. We hypothesised that hot water extracts of pea seeds stimulate germination of the orchid Ophrys apifera in vitro, particularly for extractions made during later pea development. Pea seeds, exposed to 0, 3, 6, 9, 12 or 15 days of periodic wetting, were extracted in hot water and the extracts were added to Malmgren's growth medium. Germination of O. apifera on this medium was quantified after 7 months, stimulated by a range of pure hormones. Pea seed extract collected later in pea development (at 6-15 days) inhibited germination of O. apifera. However, extracts taken at 0 and 3 days significantly increased germination from 3.8 ± 0.32% in the control to 9.1 ± 1.84% and 7.6 ± 0.79%, respectively: increases comparable to the most effective of the pure hormones. Dried peas therefore provide an economical alternative source of germination stimulants for orchids. We briefly report how sufficient mature plants of O. apifera were produced to allow a population to be multiplied to 15 times its original size during a habitat restoration project.
AbstractList Novel methods are required to break the seed dormancy of temperate-zone orchids and aid the conservation of rare species. Zeatin is produced in increasing concentrations during the development of pea (Pisum sativum) seeds. We hypothesised that hot water extracts of pea seeds stimulate germination of the orchid Ophrys apifera in vitro , particularly for extractions made during later pea development. Pea seeds, exposed to 0, 3, 6, 9, 12 or 15 days of periodic wetting, were extracted in hot water and the extracts were added to Malmgren's growth medium. Germination of O. apifera on this medium was quantified after 7 months, stimulated by a range of pure hormones. Pea seed extract collected later in pea development (at 6–15 days) inhibited germination of O. apifera . However, extracts taken at 0 and 3 days significantly increased germination from 3.8 ± 0.32% in the control to 9.1 ± 1.84% and 7.6 ± 0.79%, respectively: increases comparable to the most effective of the pure hormones. Dried peas therefore provide an economical alternative source of germination stimulants for orchids. We briefly report how sufficient mature plants of O. apifera were produced to allow a population to be multiplied to 15 times its original size during a habitat restoration project.
Novel methods are required to break the seed dormancy of temperate-zone orchids and aid the conservation of rare species. Zeatin is produced in increasing concentrations during the development of pea (Pisum sativum) seeds. We hypothesised that hot water extracts of pea seeds stimulate germination of the orchid Ophrys apifera in vitro, particularly for extractions made during later pea development. Pea seeds, exposed to 0, 3, 6, 9, 12 or 15 days of periodic wetting, were extracted in hot water and the extracts were added to Malmgren's growth medium. Germination of O. apifera on this medium was quantified after 7 months, stimulated by a range of pure hormones. Pea seed extract collected later in pea development (at 6-15 days) inhibited germination of O. apifera. However, extracts taken at 0 and 3 days significantly increased germination from 3.8 ± 0.32% in the control to 9.1 ± 1.84% and 7.6 ± 0.79%, respectively: increases comparable to the most effective of the pure hormones. Dried peas therefore provide an economical alternative source of germination stimulants for orchids. We briefly report how sufficient mature plants of O. apifera were produced to allow a population to be multiplied to 15 times its original size during a habitat restoration project.
Author Pierce, Simon
Guidi, Valentina
Ceriani, Roberta M.
Labra, Massimo
Cerabolini, Bruno E. L.
Ferrario, Andrea
Vagge, Ilda
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Snippet Novel methods are required to break the seed dormancy of temperate-zone orchids and aid the conservation of rare species. Zeatin is produced in increasing...
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SubjectTerms Asymbiotic germination
bee orchid
coconut milk
culture media
cytokinin
dry peas
Environmental restoration
germination
habitat conservation
Hormones
in vitro
mature plants
Ophrys
Orchidaceae
Pisum sativum
Rare species
seed dormancy
seed extracts
Seeds
zeatin
Title Pea seed extracts stimulate germination of the terrestrial orchid Ophrys apifera Huds. during a habitat restoration project
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