Measurement of trimethylamine-N-oxide by stable isotope dilution liquid chromatography tandem mass spectrometry
Trimethylamine-N-oxide (TMAO) levels in blood predict future risk for major adverse cardiac events including myocardial infarction, stroke, and death. Thus, the rapid determination of circulating TMAO concentration is of clinical interest. Here we report a method to measure TMAO in biological matric...
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| Published in | Analytical biochemistry Vol. 455; pp. 35 - 40 |
|---|---|
| Main Authors | , , , , , |
| Format | Journal Article |
| Language | English |
| Published |
United States
Elsevier Inc
15.06.2014
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| Subjects | |
| Online Access | Get full text |
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| Abstract | Trimethylamine-N-oxide (TMAO) levels in blood predict future risk for major adverse cardiac events including myocardial infarction, stroke, and death. Thus, the rapid determination of circulating TMAO concentration is of clinical interest. Here we report a method to measure TMAO in biological matrices by stable isotope dilution liquid chromatography tandem mass spectrometry (LC/MS/MS) with lower and upper limits of quantification of 0.05 and >200μM, respectively. Spike and recovery studies demonstrate an accuracy at low (0.5μM), mid (5μM), and high (100μM) levels of 98.2, 97.3, and 101.6%, respectively. Additional assay performance metrics include intraday and interday coefficients of variance of <6.4 and <9.9%, respectively, across the range of TMAO levels. Stability studies reveal that TMAO in plasma is stable both during storage at −80°C for 5years and to multiple freeze thaw cycles. Fasting plasma normal range studies among apparently healthy subjects (n=349) show a range of 0.73–126μM, median (interquartile range) levels of 3.45 (2.25–5.79)μM, and increasing values with age. The LC/MS/MS-based assay reported should be of value for further studies evaluating TMAO as a risk marker and for examining the effect of dietary, pharmacologic, and environmental factors on TMAO levels. |
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| AbstractList | Trimethylamine-
N
-oxide (TMAO) levels in blood predict future risk for major adverse cardiac events including myocardial infarction, stroke and death. Thus, the rapid determination of circulating TMAO concentration is of clinical interest. Here we report a method to measure TMAO in biological matrices by stable isotope dilution liquid chromatography tandem mass spectrometry (LC/MS/MS) with lower and upper limits of quantification of 0.05 and >200 µM, respectively. Spike and recovery studies demonstrate an accuracy at low (0.5 µM), mid (5 µM) and high (100 µM) levels of 98.2%, 97.3% and 101.6%, respectively. Additional assay performance metrics include intra-day and inter-day coefficients of variance of < 6.4% and < 9.9%, respectively, across the range of TMAO levels. Stability studies reveal TMAO in plasma is stable both during storage at −80 °C for 5 years and to multiple freeze thaw cycles. Fasting plasma normal range studies among apparently healthy subjects (n=349) shows a range of 0.73 – 126 µM, median (interquartile range) levels of 3.45 (2.25–5.79) µM, and increasing values with age. The LC/MS/MS based assay reported should be of value for further studies evaluating TMAO as a risk marker and for examining the effect of dietary, pharmacologic and environmental factors on TMAO levels. Trimethylamine-N-oxide (TMAO) levels in blood predict future risk for major adverse cardiac events including myocardial infarction, stroke, and death. Thus, the rapid determination of circulating TMAO concentration is of clinical interest. Here we report a method to measure TMAO in biological matrices by stable isotope dilution liquid chromatography tandem mass spectrometry (LC/MS/MS) with lower and upper limits of quantification of 0.05 and >200μM, respectively. Spike and recovery studies demonstrate an accuracy at low (0.5μM), mid (5μM), and high (100μM) levels of 98.2, 97.3, and 101.6%, respectively. Additional assay performance metrics include intraday and interday coefficients of variance of <6.4 and <9.9%, respectively, across the range of TMAO levels. Stability studies reveal that TMAO in plasma is stable both during storage at −80°C for 5years and to multiple freeze thaw cycles. Fasting plasma normal range studies among apparently healthy subjects (n=349) show a range of 0.73–126μM, median (interquartile range) levels of 3.45 (2.25–5.79)μM, and increasing values with age. The LC/MS/MS-based assay reported should be of value for further studies evaluating TMAO as a risk marker and for examining the effect of dietary, pharmacologic, and environmental factors on TMAO levels. Trimethylamine-N-oxide (TMAO) levels in blood predict future risk for major adverse cardiac events including myocardial infarction, stroke, and death. Thus, the rapid determination of circulating TMAO concentration is of clinical interest. Here we report a method to measure TMAO in biological matrices by stable isotope dilution liquid chromatography tandem mass spectrometry (LC/MS/MS) with lower and upper limits of quantification of 0.05 and >200μM, respectively. Spike and recovery studies demonstrate an accuracy at low (0.5μM), mid (5μM), and high (100μM) levels of 98.2, 97.3, and 101.6%, respectively. Additional assay performance metrics include intraday and interday coefficients of variance of <6.4 and <9.9%, respectively, across the range of TMAO levels. Stability studies reveal that TMAO in plasma is stable both during storage at -80°C for 5years and to multiple freeze thaw cycles. Fasting plasma normal range studies among apparently healthy subjects (n=349) show a range of 0.73-126μM, median (interquartile range) levels of 3.45 (2.25-5.79)μM, and increasing values with age. The LC/MS/MS-based assay reported should be of value for further studies evaluating TMAO as a risk marker and for examining the effect of dietary, pharmacologic, and environmental factors on TMAO levels. Trimethylamine-N-oxide (TMAO) levels in blood predict future risk for major adverse cardiac events including myocardial infarction, stroke, and death. Thus, the rapid determination of circulating TMAO concentration is of clinical interest. Here we report a method to measure TMAO in biological matrices by stable isotope dilution liquid chromatography tandem mass spectrometry (LC/MS/MS) with lower and upper limits of quantification of 0.05 and >200μM, respectively. Spike and recovery studies demonstrate an accuracy at low (0.5μM), mid (5μM), and high (100μM) levels of 98.2, 97.3, and 101.6%, respectively. Additional assay performance metrics include intraday and interday coefficients of variance of <6.4 and <9.9%, respectively, across the range of TMAO levels. Stability studies reveal that TMAO in plasma is stable both during storage at -80°C for 5years and to multiple freeze thaw cycles. Fasting plasma normal range studies among apparently healthy subjects (n=349) show a range of 0.73-126μM, median (interquartile range) levels of 3.45 (2.25-5.79)μM, and increasing values with age. The LC/MS/MS-based assay reported should be of value for further studies evaluating TMAO as a risk marker and for examining the effect of dietary, pharmacologic, and environmental factors on TMAO levels.Trimethylamine-N-oxide (TMAO) levels in blood predict future risk for major adverse cardiac events including myocardial infarction, stroke, and death. Thus, the rapid determination of circulating TMAO concentration is of clinical interest. Here we report a method to measure TMAO in biological matrices by stable isotope dilution liquid chromatography tandem mass spectrometry (LC/MS/MS) with lower and upper limits of quantification of 0.05 and >200μM, respectively. Spike and recovery studies demonstrate an accuracy at low (0.5μM), mid (5μM), and high (100μM) levels of 98.2, 97.3, and 101.6%, respectively. Additional assay performance metrics include intraday and interday coefficients of variance of <6.4 and <9.9%, respectively, across the range of TMAO levels. Stability studies reveal that TMAO in plasma is stable both during storage at -80°C for 5years and to multiple freeze thaw cycles. Fasting plasma normal range studies among apparently healthy subjects (n=349) show a range of 0.73-126μM, median (interquartile range) levels of 3.45 (2.25-5.79)μM, and increasing values with age. The LC/MS/MS-based assay reported should be of value for further studies evaluating TMAO as a risk marker and for examining the effect of dietary, pharmacologic, and environmental factors on TMAO levels. |
| Author | Levison, Bruce S. Donahue, Lillian Wang, Zeneng Hazen, Jennie E. Li, Xin-Min Hazen, Stanley L. |
| AuthorAffiliation | 1 Department of Cellular & Molecular Medicine, Lerner Research Institute, Cleveland Clinic, Cleveland, OH 3 Department of Cardiovascular Medicine, Heart and Vascular Institute, Cleveland Clinic, Cleveland, OH |
| AuthorAffiliation_xml | – name: 1 Department of Cellular & Molecular Medicine, Lerner Research Institute, Cleveland Clinic, Cleveland, OH – name: 3 Department of Cardiovascular Medicine, Heart and Vascular Institute, Cleveland Clinic, Cleveland, OH |
| Author_xml | – sequence: 1 givenname: Zeneng orcidid: 0000-0002-6455-8228 surname: Wang fullname: Wang, Zeneng email: wangz2@ccf.org organization: Department of Cellular & Molecular Medicine, Lerner Research Institute, Cleveland Clinic, Cleveland, OH 44195, USA – sequence: 2 givenname: Bruce S. surname: Levison fullname: Levison, Bruce S. organization: Department of Cellular & Molecular Medicine, Lerner Research Institute, Cleveland Clinic, Cleveland, OH 44195, USA – sequence: 3 givenname: Jennie E. surname: Hazen fullname: Hazen, Jennie E. organization: Department of Cellular & Molecular Medicine, Lerner Research Institute, Cleveland Clinic, Cleveland, OH 44195, USA – sequence: 4 givenname: Lillian surname: Donahue fullname: Donahue, Lillian organization: Department of Cellular & Molecular Medicine, Lerner Research Institute, Cleveland Clinic, Cleveland, OH 44195, USA – sequence: 5 givenname: Xin-Min surname: Li fullname: Li, Xin-Min organization: Department of Cellular & Molecular Medicine, Lerner Research Institute, Cleveland Clinic, Cleveland, OH 44195, USA – sequence: 6 givenname: Stanley L. surname: Hazen fullname: Hazen, Stanley L. organization: Department of Cellular & Molecular Medicine, Lerner Research Institute, Cleveland Clinic, Cleveland, OH 44195, USA |
| BackLink | https://www.ncbi.nlm.nih.gov/pubmed/24704102$$D View this record in MEDLINE/PubMed |
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| Cites_doi | 10.1038/nm.3145 10.1006/abio.1999.4351 10.1016/j.cmet.2012.12.011 10.1128/jb.149.1.22-28.1982 10.1056/NEJMoa1109400 10.1016/j.chroma.2010.01.066 10.1016/j.ab.2010.06.038 10.1099/00221287-112-2-315 10.1016/0925-4439(91)90046-C 10.1016/0003-2697(90)90449-J 10.1016/j.freeradbiomed.2013.03.001 10.1038/nature09922 10.1161/ATVBAHA.109.185645 |
| ContentType | Journal Article |
| Copyright | 2014 Elsevier Inc. Copyright © 2014 Elsevier Inc. All rights reserved. 2014 Elsevier Inc. All rights reserved. 2014 |
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| References | Tang, Wang, Levison, Koeth, Britt, Fu, Wu, Hazen (b0015) 2013; 368 Mamer, Choiniere, Treacy (b0025) 1999; 276 Stenberg, Styrvold, Strom (b0065) 1982; 149 Koeth, Wang, Levison, Buffa, Org, Sheehy, Britt, Fu, Wu, Li, Smith, DiDonato, Chen, Li, Wu, Lewis, Warrier, Brown, Krauss, Tang, Bushman, Lusis, Hazen (b0010) 2013; 19 Erupe, Liberman-Martin, Silva, Malloy, Yonis, Cocker, Purvis-Roberts (b0035) 2010; 1217 Strom, Olafsen, Larsen (b0060) 1979; 112 Wang, Klipfell, Bennett, Koeth, Levison, Dugar, Feldstein, Britt, Fu, Chung, Wu, Schauer, Smith, Allayee, Tang, DiDonato, Lusis, Hazen (b0005) 2011; 472 Bennett, de Aguiar Vallim, Wang, Shih, Meng, Gregory, Allayee, Lee, Graham, Crooke, Edwards, Hazen, Lusis (b0045) 2013; 17 daCosta, Vrbanac, Zeisel (b0070) 1990; 187 Levison, Zhang, Wang, Fu, DiDonato, Hazen (b0050) 2013; 59 Wang, Tang, Cho, Brennan, Hazen (b0055) 2009; 29 Mamer, Choiniere, Lesimple (b0030) 2010; 406 Bell, Lee, Lee, Sadler, Wilkie, Woodham (b0040) 1991; 1096 Tang (10.1016/j.ab.2014.03.016_b0015) 2013; 368 Strom (10.1016/j.ab.2014.03.016_b0060) 1979; 112 Wang (10.1016/j.ab.2014.03.016_b0005) 2011; 472 Mamer (10.1016/j.ab.2014.03.016_b0030) 2010; 406 Bell (10.1016/j.ab.2014.03.016_b0040) 1991; 1096 Stenberg (10.1016/j.ab.2014.03.016_b0065) 1982; 149 Erupe (10.1016/j.ab.2014.03.016_b0035) 2010; 1217 Levison (10.1016/j.ab.2014.03.016_b0050) 2013; 59 Wang (10.1016/j.ab.2014.03.016_b0055) 2009; 29 Koeth (10.1016/j.ab.2014.03.016_b0010) 2013; 19 daCosta (10.1016/j.ab.2014.03.016_b0070) 1990; 187 Mamer (10.1016/j.ab.2014.03.016_b0025) 1999; 276 Bennett (10.1016/j.ab.2014.03.016_b0045) 2013; 17 20599641 - Anal Biochem. 2010 Nov 1;406(1):80-2 2382825 - Anal Biochem. 1990 Jun;187(2):234-9 479836 - J Gen Microbiol. 1979 Jun;112(2):315-20 23563705 - Nat Med. 2013 May;19(5):576-85 23614584 - N Engl J Med. 2013 Apr 25;368(17):1575-84 6798018 - J Bacteriol. 1982 Jan;149(1):22-8 10603236 - Anal Biochem. 1999 Dec 15;276(2):144-9 2001424 - Biochim Biophys Acta. 1991 Feb 22;1096(2):101-7 20181351 - J Chromatogr A. 2010 Mar 26;1217(13):2070-3 23312283 - Cell Metab. 2013 Jan 8;17(1):49-60 21475195 - Nature. 2011 Apr 7;472(7341):57-63 23499838 - Free Radic Biol Med. 2013 Jun;59:2-13 19542023 - Arterioscler Thromb Vasc Biol. 2009 Sep;29(9):1383-91 |
| References_xml | – volume: 149 start-page: 22 year: 1982 end-page: 28 ident: b0065 article-title: Trimethylamine oxide respiration in publication-title: J. Bacteriol. – volume: 276 start-page: 144 year: 1999 end-page: 149 ident: b0025 article-title: Measurement of trimethylamine and trimethylamine N-oxide independently in urine by fast atom bombardment mass spectrometry publication-title: Anal. Biochem. – volume: 1217 start-page: 2070 year: 2010 end-page: 2073 ident: b0035 article-title: Determination of methylamines and trimethylamine-N-oxide in particulate matter by non-suppressed ion chromatography publication-title: J. Chromatogr. A – volume: 1096 start-page: 101 year: 1991 end-page: 107 ident: b0040 article-title: Nuclear magnetic resonance studies of blood plasma and urine from subjects with chronic renal failure: identification of trimethylamine-N-oxide publication-title: Biochim. Biophys. Acta – volume: 112 start-page: 315 year: 1979 end-page: 320 ident: b0060 article-title: Trimethylamine oxide: a terminal electron acceptor in anaerobic respiration of bacteria publication-title: J. Gen. Microbiol. – volume: 17 start-page: 49 year: 2013 end-page: 60 ident: b0045 article-title: Trimethylamine-N-oxide, a metabolite associated with atherosclerosis, exhibits complex genetic and dietary regulation publication-title: Cell Metab. – volume: 59 start-page: 2 year: 2013 end-page: 13 ident: b0050 article-title: Quantification of fatty acid oxidation products using online high-performance liquid chromatography tandem mass spectrometry publication-title: Free Radic. Biol. Med. – volume: 472 start-page: 57 year: 2011 end-page: 63 ident: b0005 article-title: Gut flora metabolism of phosphatidylcholine promotes cardiovascular disease publication-title: Nature – volume: 187 start-page: 234 year: 1990 end-page: 239 ident: b0070 article-title: The measurement of dimethylamine, trimethylamine, and trimethylamine N-oxide using capillary gas chromatography–mass spectrometry publication-title: Anal. Biochem. – volume: 19 start-page: 576 year: 2013 end-page: 585 ident: b0010 article-title: Intestinal microbiota metabolism of publication-title: Nat. Med. – volume: 368 start-page: 1575 year: 2013 end-page: 1584 ident: b0015 article-title: Intestinal microbial metabolism of phosphatidylcholine and cardiovascular risk publication-title: N. Engl. J. Med. – volume: 29 start-page: 1383 year: 2009 end-page: 1391 ident: b0055 article-title: Targeted metabolomic evaluation of arginine methylation and cardiovascular risks: potential mechanisms beyond nitric oxide synthase inhibition publication-title: Arterioscler. Thromb. Vasc. Biol. – volume: 406 start-page: 80 year: 2010 end-page: 82 ident: b0030 article-title: Measurement of urinary trimethylamine and trimethylamime oxide by direct infusion electrospray quadrupole time-of-flight mass spectrometry publication-title: Anal. Biochem. – volume: 19 start-page: 576 year: 2013 ident: 10.1016/j.ab.2014.03.016_b0010 article-title: Intestinal microbiota metabolism of l-carnitine, a nutrient in red meat, promotes atherosclerosis publication-title: Nat. Med. doi: 10.1038/nm.3145 – volume: 276 start-page: 144 year: 1999 ident: 10.1016/j.ab.2014.03.016_b0025 article-title: Measurement of trimethylamine and trimethylamine N-oxide independently in urine by fast atom bombardment mass spectrometry publication-title: Anal. Biochem. doi: 10.1006/abio.1999.4351 – volume: 17 start-page: 49 year: 2013 ident: 10.1016/j.ab.2014.03.016_b0045 article-title: Trimethylamine-N-oxide, a metabolite associated with atherosclerosis, exhibits complex genetic and dietary regulation publication-title: Cell Metab. doi: 10.1016/j.cmet.2012.12.011 – volume: 149 start-page: 22 year: 1982 ident: 10.1016/j.ab.2014.03.016_b0065 article-title: Trimethylamine oxide respiration in Proteus sp. strain NTHC153: electron transfer-dependent phosphorylation and l-serine transport publication-title: J. Bacteriol. doi: 10.1128/jb.149.1.22-28.1982 – volume: 368 start-page: 1575 year: 2013 ident: 10.1016/j.ab.2014.03.016_b0015 article-title: Intestinal microbial metabolism of phosphatidylcholine and cardiovascular risk publication-title: N. Engl. J. Med. doi: 10.1056/NEJMoa1109400 – volume: 1217 start-page: 2070 year: 2010 ident: 10.1016/j.ab.2014.03.016_b0035 article-title: Determination of methylamines and trimethylamine-N-oxide in particulate matter by non-suppressed ion chromatography publication-title: J. Chromatogr. A doi: 10.1016/j.chroma.2010.01.066 – volume: 406 start-page: 80 year: 2010 ident: 10.1016/j.ab.2014.03.016_b0030 article-title: Measurement of urinary trimethylamine and trimethylamime oxide by direct infusion electrospray quadrupole time-of-flight mass spectrometry publication-title: Anal. Biochem. doi: 10.1016/j.ab.2010.06.038 – volume: 112 start-page: 315 year: 1979 ident: 10.1016/j.ab.2014.03.016_b0060 article-title: Trimethylamine oxide: a terminal electron acceptor in anaerobic respiration of bacteria publication-title: J. Gen. Microbiol. doi: 10.1099/00221287-112-2-315 – volume: 1096 start-page: 101 year: 1991 ident: 10.1016/j.ab.2014.03.016_b0040 article-title: Nuclear magnetic resonance studies of blood plasma and urine from subjects with chronic renal failure: identification of trimethylamine-N-oxide publication-title: Biochim. Biophys. Acta doi: 10.1016/0925-4439(91)90046-C – volume: 187 start-page: 234 year: 1990 ident: 10.1016/j.ab.2014.03.016_b0070 article-title: The measurement of dimethylamine, trimethylamine, and trimethylamine N-oxide using capillary gas chromatography–mass spectrometry publication-title: Anal. Biochem. doi: 10.1016/0003-2697(90)90449-J – volume: 59 start-page: 2 year: 2013 ident: 10.1016/j.ab.2014.03.016_b0050 article-title: Quantification of fatty acid oxidation products using online high-performance liquid chromatography tandem mass spectrometry publication-title: Free Radic. Biol. Med. doi: 10.1016/j.freeradbiomed.2013.03.001 – volume: 472 start-page: 57 year: 2011 ident: 10.1016/j.ab.2014.03.016_b0005 article-title: Gut flora metabolism of phosphatidylcholine promotes cardiovascular disease publication-title: Nature doi: 10.1038/nature09922 – volume: 29 start-page: 1383 year: 2009 ident: 10.1016/j.ab.2014.03.016_b0055 article-title: Targeted metabolomic evaluation of arginine methylation and cardiovascular risks: potential mechanisms beyond nitric oxide synthase inhibition publication-title: Arterioscler. Thromb. Vasc. Biol. doi: 10.1161/ATVBAHA.109.185645 – reference: 19542023 - Arterioscler Thromb Vasc Biol. 2009 Sep;29(9):1383-91 – reference: 2382825 - Anal Biochem. 1990 Jun;187(2):234-9 – reference: 20599641 - Anal Biochem. 2010 Nov 1;406(1):80-2 – reference: 479836 - J Gen Microbiol. 1979 Jun;112(2):315-20 – reference: 23312283 - Cell Metab. 2013 Jan 8;17(1):49-60 – reference: 2001424 - Biochim Biophys Acta. 1991 Feb 22;1096(2):101-7 – reference: 21475195 - Nature. 2011 Apr 7;472(7341):57-63 – reference: 23614584 - N Engl J Med. 2013 Apr 25;368(17):1575-84 – reference: 23563705 - Nat Med. 2013 May;19(5):576-85 – reference: 6798018 - J Bacteriol. 1982 Jan;149(1):22-8 – reference: 10603236 - Anal Biochem. 1999 Dec 15;276(2):144-9 – reference: 23499838 - Free Radic Biol Med. 2013 Jun;59:2-13 – reference: 20181351 - J Chromatogr A. 2010 Mar 26;1217(13):2070-3 |
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| Snippet | Trimethylamine-N-oxide (TMAO) levels in blood predict future risk for major adverse cardiac events including myocardial infarction, stroke, and death. Thus,... Trimethylamine- N -oxide (TMAO) levels in blood predict future risk for major adverse cardiac events including myocardial infarction, stroke and death. Thus,... |
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| SubjectTerms | Adult Aged blood Cardiovascular disease Chromatography, Liquid - methods death Deuterium environmental factors Fasting Female freeze-thaw cycles Humans Indicator Dilution Techniques isotope dilution technique Limit of Detection liquid chromatography Male Mass spectrometry Methylamines - blood Middle Aged myocardial infarction Reference Values Reproducibility of Results risk factors Spectrometry, Mass, Electrospray Ionization - methods stable isotopes storage temperature stroke tandem mass spectrometry Tandem Mass Spectrometry - methods Trimethylamine-N-oxide |
| Title | Measurement of trimethylamine-N-oxide by stable isotope dilution liquid chromatography tandem mass spectrometry |
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