A humanized MDCK cell line for the efficient isolation and propagation of human influenza viruses

Here, we developed hCK, a Madin-Darby canine kidney (MDCK) cell line that expresses high levels of human influenza virus receptors and low levels of avian virus receptors. hCK cells supported human A/H3N2 influenza virus isolation and growth much more effectively than conventional MDCK or human viru...

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Published inNature microbiology Vol. 4; no. 8; pp. 1268 - 1273
Main Authors Takada, Kosuke, Kawakami, Chiharu, Fan, Shufang, Chiba, Shiho, Zhong, Gongxun, Gu, Chunyang, Shimizu, Kohei, Takasaki, Sara, Sakai-Tagawa, Yuko, Lopes, Tiago J. S., Dutta, Jayeeta, Khan, Zenab, Kriti, Divya, van Bakel, Harm, Yamada, Shinya, Watanabe, Tokiko, Imai, Masaki, Kawaoka, Yoshihiro
Format Journal Article
LanguageEnglish
Published London Nature Publishing Group UK 01.08.2019
Nature Publishing Group
Subjects
631/326/596/1578
692/699/255/1578
Animals
Antigens, CD - metabolism
Biomedical and Life Sciences
Brief Communication
Cell Line
Dogs
Humans
Infectious Diseases
Influenza
Influenza A Virus, H3N2 Subtype - genetics
Influenza A Virus, H3N2 Subtype - growth & development
Influenza A Virus, H3N2 Subtype - isolation & purification
Influenza, Human
Life Sciences
Madin Darby Canine Kidney Cells - virology
Medical Microbiology
Microbiology
Mutation
Orthomyxoviridae
Orthomyxoviridae - genetics
Orthomyxoviridae - isolation & purification
Parasitology
Receptors, Virus - genetics
Receptors, Virus - metabolism
Sialyltransferases - genetics
Sialyltransferases - metabolism
Virology
Viruses
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Abstract Here, we developed hCK, a Madin-Darby canine kidney (MDCK) cell line that expresses high levels of human influenza virus receptors and low levels of avian virus receptors. hCK cells supported human A/H3N2 influenza virus isolation and growth much more effectively than conventional MDCK or human virus receptor-overexpressing (AX4) cells. A/H3N2 viruses propagated in hCK cells also maintained higher genetic stability than those propagated in MDCK and AX4 cells. The authors report the development of a Madin-Darby canine kidney (MDCK) cell line that is more suitable than conventional MDCK or human virus receptor-overexpressing cells for the isolation and propagation of human influenza viruses without cell culture-adaptive mutations.
AbstractList Here, we developed hCK, a Madin-Darby canine kidney (MDCK) cell line that expresses high levels of human influenza virus receptors and low levels of avian virus receptors. hCK cells supported human A/H3N2 influenza virus isolation and growth much more effectively than conventional MDCK or human virus receptor-overexpressing (AX4) cells. A/H3N2 viruses propagated in hCK cells also maintained higher genetic stability than those propagated in MDCK and AX4 cells. The authors report the development of a Madin-Darby canine kidney (MDCK) cell line that is more suitable than conventional MDCK or human virus receptor-overexpressing cells for the isolation and propagation of human influenza viruses without cell culture-adaptive mutations.
Here, we developed hCK, a Madin-Darby canine kidney (MDCK) cell line that expresses high levels of human influenza virus receptors and low levels of avian virus receptors. hCK cells supported human A/H3N2 influenza virus isolation and growth much more effectively than conventional MDCK or human virus receptor-overexpressing (AX4) cells. A/H3N2 viruses propagated in hCK cells also maintained higher genetic stability than those propagated in MDCK and AX4 cells.Here, we developed hCK, a Madin-Darby canine kidney (MDCK) cell line that expresses high levels of human influenza virus receptors and low levels of avian virus receptors. hCK cells supported human A/H3N2 influenza virus isolation and growth much more effectively than conventional MDCK or human virus receptor-overexpressing (AX4) cells. A/H3N2 viruses propagated in hCK cells also maintained higher genetic stability than those propagated in MDCK and AX4 cells.
Here, we developed hCK, a Madin-Darby canine kidney (MDCK) cell line that expresses high levels of human influenza virus receptors and low levels of avian virus receptors. hCK cells supported human A/H3N2 influenza virus isolation and growth much more effectively than conventional MDCK or human virus receptor-overexpressing (AX4) cells. A/H3N2 viruses propagated in hCK cells also maintained higher genetic stability than those propagated in MDCK and AX4 cells.The authors report the development of a Madin-Darby canine kidney (MDCK) cell line that is more suitable than conventional MDCK or human virus receptor-overexpressing cells for the isolation and propagation of human influenza viruses without cell culture-adaptive mutations.
Here, we developed hCK, a Madin-Darby canine kidney (MDCK) cell line that expresses high levels of human influenza virus receptors and low levels of avian virus receptors. hCK cells supported human A/H3N2 influenza virus isolation and growth much more effectively than conventional MDCK or human virus receptor-overexpressing (AX4) cells. A/H3N2 viruses propagated in hCK cells also maintained higher genetic stability than those propagated in MDCK and AX4 cells.
Author Kawakami, Chiharu
Lopes, Tiago J. S.
Takasaki, Sara
Watanabe, Tokiko
Takada, Kosuke
Shimizu, Kohei
Khan, Zenab
Kriti, Divya
Kawaoka, Yoshihiro
Zhong, Gongxun
Sakai-Tagawa, Yuko
Dutta, Jayeeta
van Bakel, Harm
Imai, Masaki
Fan, Shufang
Gu, Chunyang
Yamada, Shinya
Chiba, Shiho
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  organization: Division of Virology, Department of Microbiology and Immunology, Institute of Medical Science, University of Tokyo, Influenza Research Institute, Department of Pathobiological Sciences, School of Veterinary Medicine, University of Wisconsin-Madison, Department of Special Pathogens, International Research Center for Infectious Diseases, Institute of Medical Science, University of Tokyo
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Snippet Here, we developed hCK, a Madin-Darby canine kidney (MDCK) cell line that expresses high levels of human influenza virus receptors and low levels of avian...
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SubjectTerms 631/326/596/1578
692/699/255/1578
Animals
Antigens, CD - metabolism
Biomedical and Life Sciences
Brief Communication
Cell Line
Dogs
Humans
Infectious Diseases
Influenza
Influenza A Virus, H3N2 Subtype - genetics
Influenza A Virus, H3N2 Subtype - growth & development
Influenza A Virus, H3N2 Subtype - isolation & purification
Influenza, Human
Life Sciences
Madin Darby Canine Kidney Cells - virology
Medical Microbiology
Microbiology
Mutation
Orthomyxoviridae
Orthomyxoviridae - genetics
Orthomyxoviridae - isolation & purification
Parasitology
Receptors, Virus - genetics
Receptors, Virus - metabolism
Sialyltransferases - genetics
Sialyltransferases - metabolism
Virology
Viruses
Title A humanized MDCK cell line for the efficient isolation and propagation of human influenza viruses
URI https://link.springer.com/article/10.1038/s41564-019-0433-6
https://www.ncbi.nlm.nih.gov/pubmed/31036910
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