8-Oxo-7,8-dihydroguanine and 8-oxo-7,8-dihydro-2′-deoxyguanosine concentrations in various human body fluids: implications for their measurement and interpretation

• Published in: Archives of toxicology Vol. 89; no. 2; pp. 201 - 210
• Main Authors: Hu, Chiung-Wen, Cooke, Marcus S., Tsai, Yi-Hung, Chao, Mu-Rong
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer Berlin Heidelberg 01.02.2015; Springer Nature B.V
• Subjects: Adult; Analytical Toxiciology; Biomedical and Life Sciences; Biomedicine; Body fluids; Chromatography, Liquid; Deoxyadenosines - analysis; Deoxyadenosines - blood; Deoxyadenosines - urine; Deoxyribonucleic acid; DNA; Environmental Health; Guanine - analogs & derivatives; Guanine - analysis; Guanine - blood; Guanine - urine; Humans; Occupational Medicine/Industrial Medicine; Pharmacology/Toxicology; Plasma; Saliva - chemistry; Solid Phase Extraction; Tandem Mass Spectrometry; Toxicology; Transport mechanism; Plasma; Online SPE LC; Oxidatively damaged DNA; MS/MS; Saliva; Deproteinization

8-Oxo-7,8-dihydro-2′-deoxyguanosine (8-oxodGuo) is the most investigated product of oxidatively damaged DNA lesion that has been associated with the development of aging, cancer and some degenerative diseases. Here, we present the first liquid chromatography–tandem mass spectrometry method that enab...