Characterization of an ecto-5 ′-nucleotidase (EC 3.1.3.5) activity in intact cells of Trichomonas vaginalis

• Published in: Experimental parasitology Vol. 105; no. 2; pp. 167 - 173
• Main Authors: Tasca, Tiana, Bonan, Carla D, Carli, Geraldo A.De, Battastini, Ana M.O, Sarkis, João J.F
• Format: Journal Article
• Language: English
• Published: San Diego, CA Elsevier Inc 01.10.2003; Elsevier
• Subjects: 5'-Nucleotidase - antagonists & inhibitors; 5'-Nucleotidase - metabolism; Adenosine Monophosphate - metabolism; Alkaline Phosphatase - antagonists & inhibitors; Animals; Biochemistry. Physiology. Immunology. Molecular biology; Biological and medical sciences; Calcium - metabolism; Experimental protozoal diseases and models; Fundamental and applied biological sciences. Psychology; Hydrogen-Ion Concentration; Hydrolysis; Infectious diseases; Kinetics; Magnesium - metabolism; Medical sciences; Parasitic diseases; Protozoa; Protozoal diseases; Trichomonas vaginalis - enzymology; Characterization; Trichomonas vaginalis; Parasite; Protozoa; Activity; Trichomonadida

The enzymatic properties of an ecto-5 ′-nucleotidase were described in Trichomonas vaginalis. The enzyme hydrolyzes nucleoside monophosphates in optimum pH values of 7.5 and 6.5 for the 30236 strain and for the 30238 strain, respectively. Mg 2+ and Ca 2+ were activators of AMP hydrolysis in both str...