Yellow fever 17D virus: pseudo-revertant suppression of defective virus penetration and spread by mutations in domains II and III of the E protein

• Published in: Virology (New York, N.Y.) Vol. 327; no. 1; pp. 41 - 49
• Main Authors: Vlaycheva, Leonssia, Nickells, Michael, Droll, Deborah A., Chambers, Thomas J.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 15.09.2004
• Subjects: Amino Acid Sequence; Animals; Cell penetration; Cercopithecus aethiops; Defective Viruses - genetics; Defective Viruses - pathogenicity; Defective Viruses - physiology; E protein; Models, Molecular; Molecular Sequence Data; Mutation; Suppression, Genetic; Vero Cells; Viral Envelope Proteins - chemistry; Viral Envelope Proteins - genetics; Viral Envelope Proteins - metabolism; Viral Plaque Assay; Yellow fever 17D virus; Yellow fever virus; Yellow fever virus - genetics; Yellow fever virus - pathogenicity; Yellow fever virus - physiology; Cell penetration; E protein; Yellow fever 17D virus

A yellow fever (YFV) 17D virus variant, which causes persistent infection of mouse neuroblastoma cells associated with defective cell penetration and small plaque size, yielded plaque-revertant viruses from cells transfected with viral transcripts encoding the adaptive mutation (Gly 360 in the E pro...