In vivo longitudinal visualization of bone marrow engraftment process in mouse calvaria using two-photon microscopy

• Published in: Scientific reports Vol. 7; no. 1; p. 44097
• Main Authors: Le, Viet-Hoan, Lee, Seunghun, Lee, Seungwon, Wang, Taejun, Hyuk Jang, Won, Yoon, Yeoreum, Kwon, Soonjae, Kim, Hyekang, Lee, Seung-Woo, Hean Kim, Ki
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 09.03.2017; Nature Publishing Group
• Subjects: 13/100; 631/1647/328/2057; 631/532/1542; 64/110; Animals; Bone marrow; Bone Marrow - metabolism; Bone Marrow - pathology; Calvaria; Dental cement; Dental Cements - pharmacology; Engraftment; Female; Humanities and Social Sciences; Mice; Microscopy; Microscopy, Fluorescence, Multiphoton - methods; multidisciplinary; Osteogenesis; Rodents; Science; Skull - injuries; Skull - metabolism; Skull - pathology; Stem cell transplantation; Stem cells; Transplantation

Intravital microscopy of mouse calvarial bone marrow (BM) is a powerful method for studying hematopoietic stem cells (HSCs) and the BM microenvironment at the cellular level. However, the current method used to access the mouse calvaria allows for only a few imaging times in the same mouse because o...