Comparison of antimicrobial peptide purification via free‐flow electrophoresis and gel filtration chromatography

• Published in: Electrophoresis Vol. 38; no. 24; pp. 3147 - 3154
• Main Authors: Xia, Zhi‐Jun, Liu, Zhen, Kong, Fan‐Zhi, Fan, Liu‐Yin, Xiao, Hua, Cao, Cheng‐Xi
• Format: Journal Article
• Language: English
• Published: Germany Wiley Subscription Services, Inc 01.12.2017
• Subjects: Agar; Antiinfectives and antibacterials; Antimicrobial agents; Antimicrobial peptides; antimicrobial properties; Biomolecules; Chromatography; culture media; Dilution; Electrophoresis; Fermentation; Filtration; Free‐flow electrophoresis; Gel chromatography; Gel filtration; Gel filtration chromatography; gels; molecular weight; pathogens; Peptides; Physiochemistry; polyacrylamide gel electrophoresis; Yeast; yeasts; Gel filtration chromatography; Antimicrobial peptides; Free-flow electrophoresis

Antimicrobial peptides (AMPs) are usually small and cationic biomolecules with broad‐spectrum antimicrobial activities against pathogens. Purifying them from complex samples is essential to study their physiochemical properties. In this work, free‐flow zone electrophoresis (FFZE) was utilized to pur...