Analysing cell-free plasma DNA and SLE disease activity
Eur J Clin Invest 2011; 41 (6): 579–583 Background Over the years, the demonstration and confirmation of cell‐free DNA in the circulation has increasingly been recognized as a valuable diagnostic tool. Likewise, it has been known for some time that DNA structures that are targeted by auto‐antibodie...
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| Published in | European journal of clinical investigation Vol. 41; no. 6; pp. 579 - 583 |
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| Main Authors | , , , , , , , |
| Format | Journal Article |
| Language | English |
| Published |
Oxford, UK
Blackwell Publishing Ltd
01.06.2011
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| Abstract | Eur J Clin Invest 2011; 41 (6): 579–583
Background Over the years, the demonstration and confirmation of cell‐free DNA in the circulation has increasingly been recognized as a valuable diagnostic tool. Likewise, it has been known for some time that DNA structures that are targeted by auto‐antibodies play a central role in systemic lupus erythematosis (SLE) and that DNA‐antibody complexes in the circulation are one of the hallmarks of SLE. Investigating whether and to what degree fluctuations in free plasma DNA levels in patients with SLE might correspond to disease severity was therefore the goal of this investigation.
Methods Blood from 13 patients with SLE and from 13 healthy controls was taken and analysed for the presence of anti‐dsDNA, anti‐ssDNA, anti‐nucleosome, anti‐histone antibodies as well as for cell‐free DNA concentrations. For each patient, the SLE disease activity index (SLEDAI) was calculated.
Results As demonstrated herein, compared to healthy subjects, cell‐free DNA plasma levels in patients with SLE were significantly increased and so were anti‐dsDNA, anti‐ssDNA, anti‐histone and anti‐nucleosome antibodies. Furthermore, a statistically significant correlation was noted between cell‐free DNA and anti‐histone antibodies in patients with SLE. However, no correlation was noted between disease activity and anti‐dsDNA, anti‐ssDNA and anti‐nucleosome antibody concentrations. Surprisingly, and more important in the context of this study, there was no correlation between cell‐free DNA levels and SLEDAI scores.
Conclusions The presented data seem to exclude measuring free plasma DNA as an inexpensive, simple and quick tool to assess disease activity in patients with SLE. Further studies on a larger patient population would be needed to confirm our results. |
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| AbstractList | Eur J Clin Invest 2011; 41 (6): 579–583
Background Over the years, the demonstration and confirmation of cell‐free DNA in the circulation has increasingly been recognized as a valuable diagnostic tool. Likewise, it has been known for some time that DNA structures that are targeted by auto‐antibodies play a central role in systemic lupus erythematosis (SLE) and that DNA‐antibody complexes in the circulation are one of the hallmarks of SLE. Investigating whether and to what degree fluctuations in free plasma DNA levels in patients with SLE might correspond to disease severity was therefore the goal of this investigation.
Methods Blood from 13 patients with SLE and from 13 healthy controls was taken and analysed for the presence of anti‐dsDNA, anti‐ssDNA, anti‐nucleosome, anti‐histone antibodies as well as for cell‐free DNA concentrations. For each patient, the SLE disease activity index (SLEDAI) was calculated.
Results As demonstrated herein, compared to healthy subjects, cell‐free DNA plasma levels in patients with SLE were significantly increased and so were anti‐dsDNA, anti‐ssDNA, anti‐histone and anti‐nucleosome antibodies. Furthermore, a statistically significant correlation was noted between cell‐free DNA and anti‐histone antibodies in patients with SLE. However, no correlation was noted between disease activity and anti‐dsDNA, anti‐ssDNA and anti‐nucleosome antibody concentrations. Surprisingly, and more important in the context of this study, there was no correlation between cell‐free DNA levels and SLEDAI scores.
Conclusions The presented data seem to exclude measuring free plasma DNA as an inexpensive, simple and quick tool to assess disease activity in patients with SLE. Further studies on a larger patient population would be needed to confirm our results. Background Over the years, the demonstration and confirmation of cell-free DNA in the circulation has increasingly been recognized as a valuable diagnostic tool. Likewise, it has been known for some time that DNA structures that are targeted by auto-antibodies play a central role in systemic lupus erythematosis (SLE) and that DNA-antibody complexes in the circulation are one of the hallmarks of SLE. Investigating whether and to what degree fluctuations in free plasma DNA levels in patients with SLE might correspond to disease severity was therefore the goal of this investigation. Methods Blood from 13 patients with SLE and from 13 healthy controls was taken and analysed for the presence of anti-dsDNA, anti-ssDNA, anti-nucleosome, anti-histone antibodies as well as for cell-free DNA concentrations. For each patient, the SLE disease activity index (SLEDAI) was calculated. Results As demonstrated herein, compared to healthy subjects, cell-free DNA plasma levels in patients with SLE were significantly increased and so were anti-dsDNA, anti-ssDNA, anti-histone and anti-nucleosome antibodies. Furthermore, a statistically significant correlation was noted between cell-free DNA and anti-histone antibodies in patients with SLE. However, no correlation was noted between disease activity and anti-dsDNA, anti-ssDNA and anti-nucleosome antibody concentrations. Surprisingly, and more important in the context of this study, there was no correlation between cell-free DNA levels and SLEDAI scores. Conclusions The presented data seem to exclude measuring free plasma DNA as an inexpensive, simple and quick tool to assess disease activity in patients with SLE. Further studies on a larger patient population would be needed to confirm our results.Original Abstract: Eur J Clin Invest 2011; 41 (6): 579-583 Over the years, the demonstration and confirmation of cell-free DNA in the circulation has increasingly been recognized as a valuable diagnostic tool. Likewise, it has been known for some time that DNA structures that are targeted by auto-antibodies play a central role in systemic lupus erythematosis (SLE) and that DNA-antibody complexes in the circulation are one of the hallmarks of SLE. Investigating whether and to what degree fluctuations in free plasma DNA levels in patients with SLE might correspond to disease severity was therefore the goal of this investigation.BACKGROUNDOver the years, the demonstration and confirmation of cell-free DNA in the circulation has increasingly been recognized as a valuable diagnostic tool. Likewise, it has been known for some time that DNA structures that are targeted by auto-antibodies play a central role in systemic lupus erythematosis (SLE) and that DNA-antibody complexes in the circulation are one of the hallmarks of SLE. Investigating whether and to what degree fluctuations in free plasma DNA levels in patients with SLE might correspond to disease severity was therefore the goal of this investigation.Blood from 13 patients with SLE and from 13 healthy controls was taken and analysed for the presence of anti-dsDNA, anti-ssDNA, anti-nucleosome, anti-histone antibodies as well as for cell-free DNA concentrations. For each patient, the SLE disease activity index (SLEDAI) was calculated.METHODSBlood from 13 patients with SLE and from 13 healthy controls was taken and analysed for the presence of anti-dsDNA, anti-ssDNA, anti-nucleosome, anti-histone antibodies as well as for cell-free DNA concentrations. For each patient, the SLE disease activity index (SLEDAI) was calculated.As demonstrated herein, compared to healthy subjects, cell-free DNA plasma levels in patients with SLE were significantly increased and so were anti-dsDNA, anti-ssDNA, anti-histone and anti-nucleosome antibodies. Furthermore, a statistically significant correlation was noted between cell-free DNA and anti-histone antibodies in patients with SLE. However, no correlation was noted between disease activity and anti-dsDNA, anti-ssDNA and anti-nucleosome antibody concentrations. Surprisingly, and more important in the context of this study, there was no correlation between cell-free DNA levels and SLEDAI scores.RESULTSAs demonstrated herein, compared to healthy subjects, cell-free DNA plasma levels in patients with SLE were significantly increased and so were anti-dsDNA, anti-ssDNA, anti-histone and anti-nucleosome antibodies. Furthermore, a statistically significant correlation was noted between cell-free DNA and anti-histone antibodies in patients with SLE. However, no correlation was noted between disease activity and anti-dsDNA, anti-ssDNA and anti-nucleosome antibody concentrations. Surprisingly, and more important in the context of this study, there was no correlation between cell-free DNA levels and SLEDAI scores. The presented data seem to exclude measuring free plasma DNA as an inexpensive, simple and quick tool to assess disease activity in patients with SLE. Further studies on a larger patient population would be needed to confirm our results.CONCLUSIONS The presented data seem to exclude measuring free plasma DNA as an inexpensive, simple and quick tool to assess disease activity in patients with SLE. Further studies on a larger patient population would be needed to confirm our results. Over the years, the demonstration and confirmation of cell-free DNA in the circulation has increasingly been recognized as a valuable diagnostic tool. Likewise, it has been known for some time that DNA structures that are targeted by auto-antibodies play a central role in systemic lupus erythematosis (SLE) and that DNA-antibody complexes in the circulation are one of the hallmarks of SLE. Investigating whether and to what degree fluctuations in free plasma DNA levels in patients with SLE might correspond to disease severity was therefore the goal of this investigation. Blood from 13 patients with SLE and from 13 healthy controls was taken and analysed for the presence of anti-dsDNA, anti-ssDNA, anti-nucleosome, anti-histone antibodies as well as for cell-free DNA concentrations. For each patient, the SLE disease activity index (SLEDAI) was calculated. As demonstrated herein, compared to healthy subjects, cell-free DNA plasma levels in patients with SLE were significantly increased and so were anti-dsDNA, anti-ssDNA, anti-histone and anti-nucleosome antibodies. Furthermore, a statistically significant correlation was noted between cell-free DNA and anti-histone antibodies in patients with SLE. However, no correlation was noted between disease activity and anti-dsDNA, anti-ssDNA and anti-nucleosome antibody concentrations. Surprisingly, and more important in the context of this study, there was no correlation between cell-free DNA levels and SLEDAI scores. The presented data seem to exclude measuring free plasma DNA as an inexpensive, simple and quick tool to assess disease activity in patients with SLE. Further studies on a larger patient population would be needed to confirm our results. |
| Author | Fodinger, Manuela Erlacher, Ludwig Stuhlmeier, Karl M. Hsiao, Yu-Yang Bernhard, Duhm Atamaniuk, Johanna Tiran, Beate Mustak, Monika |
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| BackLink | https://www.ncbi.nlm.nih.gov/pubmed/21128939$$D View this record in MEDLINE/PubMed |
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| References_xml | – reference: Saukkonen K, Lakkisto P, Pettilä V, Varpula M, Karlsson S, Ruokonen E et al. Cell-free plasma DNA as a predictor of outcome in severe sepsis and septic shock. Clin Chem 2008;54:1000-7. – reference: Lo YM. Circulating nucleic acids in plasma and serum: an overview. Ann N Y Acad Sci 2001;945:1-7. – reference: Fenton KA, Tommeras B, Marion TN, Rekvig OP. Pure anti-dsDNA mAbs need chromatin structures to promote glomerular mesangial deposits in BALB/c mice. Autoimmunity 2010;43:179-88. – reference: Schwarzenbach H, Alix-Panabiéres C, Müller I, Letang N, Vendrell JP, Rebillard X et al. Cell-free tumor DNA in blood plasma as a marker for circulating tumor cells in prostate cancer. Clin Cancer Res 2009;15:1032-8. – reference: Zanetti-Dällenbach R, Wight E, Fan AX, Lapaire O, Hahn S, Holzgreve W et al. Positive correlation of cell-free DNA in plasma/serum in patients with malignant and benign breast disease. 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Effects of ultra-marathon on circulating DNA and mRNA expression of pro- and anti-apoptotic genes in mononuclear cells. Eur J Appl Physiol 2008;24:24. – reference: Wong BC, Lo YM. Cell-free DNA and RNA in plasma as new tools for molecular diagnostics. Expert Rev Mol Diagn 2003;3:785-97. – reference: Fatouros IG, Destouni A, Margonis K, Jamurtas AZ, Vrettou C, Kouretas D et al. Cell-free plasma DNA as a novel marker of aseptic inflammation severity related to exercise overtraining. Clin Chem 2006;52:1820-4. – reference: Conrad K, Ittenson A, Reinhold D, Fischer R, Roggenbuck D, Büttner T et al. High sensitive detection of double-stranded DNA autoantibodies by a modified Crithidia luciliae immunofluorescence test. Ann N Y Acad Sci 2009;1173:180-5. – reference: Heidenreich U, Mayer G, Herold M, Klotz W, Stempfl Al-Jazrawi K, Lhotta K. Sensitivity and specificity of autoantibody tests in the differential diagnosis of lupus nephritis. 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| Snippet | Eur J Clin Invest 2011; 41 (6): 579–583
Background Over the years, the demonstration and confirmation of cell‐free DNA in the circulation has increasingly... Over the years, the demonstration and confirmation of cell-free DNA in the circulation has increasingly been recognized as a valuable diagnostic tool.... Background Over the years, the demonstration and confirmation of cell-free DNA in the circulation has increasingly been recognized as a valuable diagnostic... |
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| SubjectTerms | Anti-DNA antibodies Antibodies Antibodies, Antinuclear - analysis Antibodies, Antinuclear - immunology Autoantibodies Blood Case-Control Studies Cell-free DNA Data processing DNA - analysis DNA - blood DNA - immunology DNA structure Enzyme-Linked Immunosorbent Assay Histones - immunology Humans Lupus Erythematosus, Systemic - blood Lupus Erythematosus, Systemic - immunology Nucleosomes - immunology Plasma - immunology Plasma levels Severity of Illness Index SLE disease activity index score Statistical analysis Statistics as Topic systemic lupus erythematosis |
| Title | Analysing cell-free plasma DNA and SLE disease activity |
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