Sensitive CE‐MS method for monitoring of riociguat and desmethylriociguat levels in human serum

• Published in: Electrophoresis Vol. 41; no. 18-19; pp. 1564 - 1567
• Main Authors: Hložek, Tomáš, Štícha, Martin, Bursová, Miroslava, Jelínek, Ivan, Tůma, Petr, Čabala, Radomír
• Format: Journal Article
• Language: English
• Published: Germany Wiley Subscription Services, Inc 01.10.2020
• Subjects: ammonium; Analytical chemistry; antihypertensive agents; Antihypertensives; blood serum; calibration; Capillary electrophoresis; chemical species; Desmethylriociguat; Determination; Electrolytes; Electrophoresis; Electrophoresis, Capillary - methods; formates; High performance liquid chromatography; Humans; Hypertension; Limit of Detection; Linear Models; Liquid-Liquid Extraction; Mass spectrometry; Optimization; Pharmacokinetics; Pharmacology; Pretreatment; Pyrazoles - blood; Pyrazoles - chemistry; Pyrazoles - isolation & purification; Pyrazoles - pharmacokinetics; Pyrimidines - blood; Pyrimidines - chemistry; Pyrimidines - isolation & purification; Pyrimidines - pharmacokinetics; Reproducibility of Results; Riociguat; Scientific imaging; Spectrometry, Mass, Electrospray Ionization - methods; Spectroscopy; Desmethylriociguat; Determination; Capillary electrophoresis; Mass spectrometry; Riociguat

Riociguat is novel antihypertensive drug for treatment of pulmonary hypertension. As such, it is still being tested in many clinical and pharmacokinetic trials. Existing methods that determine serum riociguat and desmethylriociguat (DMR) are based solely on liquid chromatography with mass spectromet...