Nested polymerase chain reaction assay for the detection of B19 parvovirus DNA in human immunodeficiency virus patients

• Published in: Journal of medical virology Vol. 40; no. 2; p. 157
• Main Authors: Musiani, M, Azzi, A, Zerbini, M, Gibellini, D, Venturoli, S, Zakrzewska, K, Re, M C, Gentilomi, G, Gallinella, G, La Placa, M
• Format: Journal Article
• Language: English
• Published: United States 01.06.1993
• Subjects: Adult; Antibodies, Viral - blood; Base Sequence; DNA, Viral - blood; DNA, Viral - genetics; Female; HIV Seropositivity - immunology; HIV Seropositivity - microbiology; HIV-1; Humans; Immunoglobulin M - blood; Male; Middle Aged; Molecular Sequence Data; Parvoviridae Infections - diagnosis; Parvovirus B19, Human - genetics; Parvovirus B19, Human - isolation & purification; Polymerase Chain Reaction - methods; Reproducibility of Results; Retrospective Studies

Persistent B19 parvovirus infection has been recognized in immunocompromised patients, often occurring with a low-titer viremia. In this study, nested polymerase chain reaction (PCR) for the detection of B19 parvovirus DNA was carried out on the sera of 49 human immunodeficiency virus (HIV)-1-seropo...