Development of a CE‐MS method for the study of riociguat and metabolite M1 in pharmaceutical analysis

• Published in: Electrophoresis Vol. 40; no. 22; pp. 2936 - 2945
• Main Authors: Štícha, Martin, Hložek, Tomáš, Bursová, Miroslava, Čabala, Radomír, Jelínek, Ivan
• Format: Journal Article
• Language: English
• Published: Germany Wiley Subscription Services, Inc 01.11.2019
• Subjects: antihypertensive agents; buffers; calibration; Capillary electrophoresis; dissociation; Dissociation constant; Electrolytes; Electrophoresis, Capillary - methods; formates; Fragmentation; Humans; Hypertension; ionic strength; Ions; Limit of Detection; Linear Models; Lithium; lithium acetate; Mass spectrometry; Mass Spectrometry - methods; Metabolites; Parameter estimation; Photometry; Pyrazoles - analysis; Pyrazoles - blood; Pyrazoles - chemistry; Pyrazoles - metabolism; Pyrimidines - analysis; Pyrimidines - blood; Pyrimidines - chemistry; Pyrimidines - metabolism; Reproducibility of Results; Riociguat; Spectrometry; Symmetry; Dissociation constant; Capillary electrophoresis; Mass spectrometry; Riociguat; Fragmentation

Riociguat is a novel antihypertensive drug for the treatment of pulmonary hypertension. We present electrophoretic characterization, i.e. migration behavior of riociguat and metabolite M1 as support for optimized CZE/MS assay. Fundamental separation parameters, such as peak width, symmetry, and reso...