Targeted activation of PKA and Epac promotes glioblastoma regression in vitro
Ras-p44/42 mitogen-activated protein kinase (MAPK) and Akt signaling are the key pathways involved in the promotion of glioblastoma formation. Notably, phosphodiesterase 4 (PDE4) is widely expressed in brain tumors and promotes their growth. PDE4 inhibitors have been reported to suppress glioblastom...
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Published in | Molecular and clinical oncology Vol. 1; no. 2; pp. 281 - 285 |
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Main Authors | , , , , , , |
Format | Journal Article |
Language | English |
Published |
England
D.A. Spandidos
01.03.2013
Spandidos Publications UK Ltd |
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Abstract | Ras-p44/42 mitogen-activated protein kinase (MAPK) and Akt signaling are the key pathways involved in the promotion of glioblastoma formation. Notably, phosphodiesterase 4 (PDE4) is widely expressed in brain tumors and promotes their growth. PDE4 inhibitors have been reported to suppress glioblastoma growth in vitro and in vivo. The mechanisms underlying these actions, however, have yet to be elucidated. The aim of this study was to investigate whether intracellular cyclic adenosine monophosphate (cAMP) was able to suppress the Ras-p44/42 MAPK signaling pathway via protein kinase A (PKA) and exchange protein directly activated by cAMP (Epac) in U87MG human malignant glioma cells. Forskolin, an activator of adenylate cyclase, inhibited cell growth and the phosphorylation of p44/42 MAPK in U87MG cells, whereas the non-hydrolyzable cAMP analog 8-bromoadenosine 3′,5′-cAMP (8-Br-cAMP) considerably suppressed cell growth and phosphorylation of p44/42 MAPK. The inhibitory effects of forskolin were partially prevented by the PKA inhibitor H89. The Epac-selective agonist 8-(4-chlorophenylthio)-2′-O-methyladenosine cAMP (8-CPT-cAMP) inhibited phosphorylation of p44/42 MAPK. These findings suggest that PKA and Epac are involved in the effect of intracellular cAMP on the Ras-p44/42 MAPK signaling pathway. |
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AbstractList | Ras-p44/42 mitogen-activated protein kinase (MAPK) and Akt signaling are the key pathways involved in the promotion of glioblastoma formation. Notably, phosphodiesterase 4 (PDE4) is widely expressed in brain tumors and promotes their growth. PDE4 inhibitors have been reported to suppress glioblastoma growth
in vitro
and
in vivo
. The mechanisms underlying these actions, however, have yet to be elucidated. The aim of this study was to investigate whether intracellular cyclic adenosine monophosphate (cAMP) was able to suppress the Ras-p44/42 MAPK signaling pathway via protein kinase A (PKA) and exchange protein directly activated by cAMP (Epac) in U87MG human malignant glioma cells. Forskolin, an activator of adenylate cyclase, inhibited cell growth and the phosphorylation of p44/42 MAPK in U87MG cells, whereas the non-hydrolyzable cAMP analog 8-bromoadenosine 3′,5′-cAMP (8-Br-cAMP) considerably suppressed cell growth and phosphorylation of p44/42 MAPK. The inhibitory effects of forskolin were partially prevented by the PKA inhibitor H89. The Epac-selective agonist 8-(4-chlorophenylthio)-2′-
O
-methyladenosine cAMP (8-CPT-cAMP) inhibited phosphorylation of p44/42 MAPK. These findings suggest that PKA and Epac are involved in the effect of intracellular cAMP on the Ras-p44/42 MAPK signaling pathway. Ras-p44/42 mitogen-activated protein kinase (MAPK) and Akt signaling are the key pathways involved in the promotion of glioblastoma formation. Notably, phosphodiesterase 4 (PDE4) is widely expressed in brain tumors and promotes their growth. PDE4 inhibitors have been reported to suppress glioblastoma growth and . The mechanisms underlying these actions, however, have yet to be elucidated. The aim of this study was to investigate whether intracellular cyclic adenosine monophosphate (cAMP) was able to suppress the Ras-p44/42 MAPK signaling pathway via protein kinase A (PKA) and exchange protein directly activated by cAMP (Epac) in U87MG human malignant glioma cells. Forskolin, an activator of adenylate cyclase, inhibited cell growth and the phosphorylation of p44/42 MAPK in U87MG cells, whereas the non-hydrolyzable cAMP analog 8-bromoadenosine 3',5'-cAMP (8-Br-cAMP) considerably suppressed cell growth and phosphorylation of p44/42 MAPK. The inhibitory effects of forskolin were partially prevented by the PKA inhibitor H89. The Epac-selective agonist 8-(4-chlorophenylthio)-2'- -methyladenosine cAMP (8-CPT-cAMP) inhibited phosphorylation of p44/42 MAPK. These findings suggest that PKA and Epac are involved in the effect of intracellular cAMP on the Ras-p44/42 MAPK signaling pathway. Ras-p44/42 mitogen-activated protein kinase (MAPK) and Akt signaling are the key pathways involved in the promotion of glioblastoma formation. Notably, phosphodiesterase 4 (PDE4) is widely expressed in brain tumors and promotes their growth. PDE4 inhibitors have been reported to suppress glioblastoma growth in vitro and in vivo. The mechanisms underlying these actions, however, have yet to be elucidated. The aim of this study was to investigate whether intracellular cyclic adenosine monophosphate (cAMP) was able to suppress the Ras-p44/42 MAPK signaling pathway via protein kinase A (PKA) and exchange protein directly activated by cAMP (Epac) in U87MG human malignant glioma cells. Forskolin, an activator of adenylate cyclase, inhibited cell growth and the phosphorylation of p44/42 MAPK in U87MG cells, whereas the non-hydrolyzable cAMP analog 8-bromoadenosine 3′,5′-cAMP (8-Br-cAMP) considerably suppressed cell growth and phosphorylation of p44/42 MAPK. The inhibitory effects of forskolin were partially prevented by the PKA inhibitor H89. The Epac-selective agonist 8-(4-chlorophenylthio)-2′-O-methyladenosine cAMP (8-CPT-cAMP) inhibited phosphorylation of p44/42 MAPK. These findings suggest that PKA and Epac are involved in the effect of intracellular cAMP on the Ras-p44/42 MAPK signaling pathway. |
Author | MIWA, SHINJI HITOMI, YOSHIAKI NAKAMURA, HIROYUKI TSUCHIYA, HIROYUKI KOIZUMI, SHOICHI YACHIE, AKIHIRO SUGIMOTO, NAOTOSHI |
AuthorAffiliation | 2 Orthopedic Surgery, Kanazawa University, Kanazawa, Ishikawa 920-8640, Japan 5 United Graduate School of Child Development, Kanazawa University, Kanazawa, Ishikawa 920-8640, Japan 3 Public Health, Kanazawa University, Kanazawa, Ishikawa 920-8640, Japan 1 Departments of Physiology, Kanazawa University, Kanazawa, Ishikawa 920-8640, Japan 4 Pediatrics, Graduate School of Medical Science, Kanazawa University, Kanazawa, Ishikawa 920-8640, Japan |
AuthorAffiliation_xml | – name: 1 Departments of Physiology, Kanazawa University, Kanazawa, Ishikawa 920-8640, Japan – name: 2 Orthopedic Surgery, Kanazawa University, Kanazawa, Ishikawa 920-8640, Japan – name: 5 United Graduate School of Child Development, Kanazawa University, Kanazawa, Ishikawa 920-8640, Japan – name: 3 Public Health, Kanazawa University, Kanazawa, Ishikawa 920-8640, Japan – name: 4 Pediatrics, Graduate School of Medical Science, Kanazawa University, Kanazawa, Ishikawa 920-8640, Japan |
Author_xml | – sequence: 1 givenname: NAOTOSHI surname: SUGIMOTO fullname: SUGIMOTO, NAOTOSHI organization: Departments of Physiology, Kanazawa University, Kanazawa, Ishikawa 920-8640, Japan – sequence: 2 givenname: SHINJI surname: MIWA fullname: MIWA, SHINJI organization: Orthopedic Surgery, Kanazawa University, Kanazawa, Ishikawa 920-8640, Japan – sequence: 3 givenname: HIROYUKI surname: TSUCHIYA fullname: TSUCHIYA, HIROYUKI organization: Orthopedic Surgery, Kanazawa University, Kanazawa, Ishikawa 920-8640, Japan – sequence: 4 givenname: YOSHIAKI surname: HITOMI fullname: HITOMI, YOSHIAKI organization: Public Health, Kanazawa University, Kanazawa, Ishikawa 920-8640, Japan – sequence: 5 givenname: HIROYUKI surname: NAKAMURA fullname: NAKAMURA, HIROYUKI organization: Public Health, Kanazawa University, Kanazawa, Ishikawa 920-8640, Japan – sequence: 6 givenname: AKIHIRO surname: YACHIE fullname: YACHIE, AKIHIRO organization: Pediatrics, Graduate School of Medical Science, Kanazawa University, Kanazawa, Ishikawa 920-8640, Japan – sequence: 7 givenname: SHOICHI surname: KOIZUMI fullname: KOIZUMI, SHOICHI organization: United Graduate School of Child Development, Kanazawa University, Kanazawa, Ishikawa 920-8640, Japan |
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Keywords | mitogen-activated protein kinase exchange protein activated by cyclic adenosine monophosphate glioblastoma cyclic adenosine monophosphate protein kinase A |
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SubjectTerms | Bone cancer Brain cancer Cell growth cyclic adenosine monophosphate exchange protein activated by cyclic adenosine monophosphate glioblastoma Kinases mitogen-activated protein kinase Oncology Phosphorylation protein kinase A Proteins Signal transduction Statistical analysis Studies Tumors Variance analysis |
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Title | Targeted activation of PKA and Epac promotes glioblastoma regression in vitro |
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