Activation and Stabilization of Lipase B from Candida antarctica by Immobilization on Polymer Brushes with Optimized Surface Structure

• Published in: Applied biochemistry and biotechnology Vol. 194; no. 8; pp. 3384 - 3399
• Main Authors: Wunschik, Dennis Sebastian, Lorenz, André, Ingenbosch, Kim Nadine, Gutmann, Jochen Stefan, Hoffmann-Jacobsen, Kerstin
• Format: Journal Article
• Language: English
• Published: New York Springer US 01.08.2022; Springer Nature B.V
• Subjects: Basidiomycota; Biocatalysis; Biocatalysts; Biochemistry; Biotechnology; Brushes; Candida antarctica; carboxylic ester hydrolases; Catalysis; Chemical synthesis; Chemistry; Chemistry and Materials Science; Core-shell particles; Crosslinking; Electron transfer; Enzymes, Immobilized - chemistry; Esterification; glutaraldehyde; heptane; Heptanes; Hydrophobicity; Immobilization; Isopropanol; Isopropyl alcohol; Lipase; Lipase - chemistry; Nanoparticles; Original; Original Article; polymerization; Polymers; Polymers - chemistry; Polymethyl Methacrylate; Polymethylmethacrylate; Pseudozyma antarctica; Silica; spectroscopy; Stability analysis; Support systems; Surface properties; Surface structure; synthesis; Transesterification; Polymer brushes; Non-native media; Enzyme immobilization; Biocatalysis; Lipase

A reusable support system for the immobilization of lipases is developed using hybrid polymer-inorganic core shell nanoparticles. The biocatalyst core consists of a silica nanoparticle. PMMA is grafted from the nanoparticle as polymer brush via ARGET ATRP (activator regenerated by electron transfer...