Species Specific Identification of Nine Human Bifidobacterium spp. in Feces

• Published in: Systematic and applied microbiology Vol. 25; no. 4; pp. 536 - 543
• Main Authors: Germond, Jacques-Edouard, Mamin, Olivia, Mollet, Beat
• Format: Journal Article
• Language: English
• Published: Jena Elsevier GmbH 01.12.2002; Elsevier; Elsevier Science Ltd
• Subjects: Adult; Bacteriological methods and techniques used in bacteriology; Bacteriology; Bifidobacteriales Infections - microbiology; Bifidobacterium - classification; Bifidobacterium - genetics; Bifidobacterium - isolation & purification; Biological and medical sciences; DNA Primers; Electrophoresis, Agar Gel - methods; feces; Feces - microbiology; Female; Fundamental and applied biological sciences. Psychology; Human Bifidobacterium spp; Humans; Intestines - microbiology; Male; Microbiology; Middle Aged; Multiplex PCR amplification; Phylogeny; Species identification; Species Specificity; Human Bifidobacterium spp; Species identification; feces; Multiplex PCR amplification; Human; Bifidobacterium; Actinomycetaceae; Multiplex polymerase chain reaction; Ribosomal DNA; Identification; Species specificity; Method; Actinomycetales; 16S-RNA; Bacteria; Actinomycetes; Feces
• ISSN: 0723-2020; 1618-0984
• DOI: 10.1078/07232020260517670

Based on the 16S rDNA sequences, species specific primers were designed for the rapid identification by DNA amplification of nine human Bifidobacterium spp., namely B. adolescentis, B. angulatum, B. bifidum, B. breve, B. catenulatum, B. dentium, B. infantis, B. longum, B. pseudocatenulatum. B. lactis currently included in dairy products was added to the series. The primers were designed to target different positions of the 16S rDNA, allowing the simultaneous identification of these ten species of Bifidobacterium using two mixtures of primers. The identification procedure described in this paper was validated by establishing a correlation with an Alu I restriction pattern of the different full length amplified 16S rDNA. This multiple primer DNA amplification technique was applied for the identification of pure colonies of Bifidobacterium spp. or directly from total bacteria recovered from human fecal samples. The technique was shown to be useful to detect dominant species and, when primers were used in separate reactions, underrepresented species could be identified as well.