Differences between Rainbow Trout and Brown Trout in the Regulation of the Pituitary–Interrenal Axis and Physiological Performance during Confinement

• Published in: General and comparative endocrinology Vol. 115; no. 2; pp. 210 - 219
• Main Authors: Ruane, N.M., Wendelaar Bonga, S.E., Balm, P.H.M.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.08.1999
• Subjects: Adrenocorticotropic Hormone - blood; alpha-MSH - blood; Animals; Blood Cell Count; Blood Glucose - metabolism; confinement; endocrine stress responses, species differences; Environment; fish immunology; Freshwater; Hydrocortisone - blood; Immunity - physiology; Kidney - physiology; Oncorhynchus mykiss; Oncorhynchus mykiss - physiology; Pituitary Gland - physiology; pituitary–interrenal; Proteins - metabolism; Salmo trutta; Species Specificity; Trout - physiology; pituitary–interrenal; fish immunology; confinement; endocrine stress responses, species differences
• ISSN: 0016-6480; 1095-6840
• DOI: 10.1006/gcen.1999.7292

The responses of rainbow trout and brown trout to the same stressor were compared by measuring primary and secondary stress responses during and after a 5.5-h net confinement. Basal levels of adrenocorticotropic hormone (ACTH), α-melanocyte-stimulating hormone (α-MSH), and glucose were higher in brown trout than in rainbow trout. While confinement induced transient increases in plasma ACTH and cortisol levels in both species, the magnitude of these responses, but not the time course, was greater in brown trout. Brown trout, but not rainbow trout, showed a reduction in plasma α-MSH levels after 5.5 h confinement before returning to control values, and the glucose levels in the brown trout were elevated throughout the confinement and recovery periods. Confinement also resulted in increased hematocrit values and reduced plasma sodium and chloride levels in both species. Rainbow trout appeared to recover faster from the confinement, as glucose and hematocrit values in the brown trout remained elevated and ionoregulatory disturbances persisted even after 46 h. During recovery effects on the immune system were more pronounced in brown trout than in rainbow trout. Circulating white blood cell numbers were reduced in both species following 23 h recovery, but remained low in the brown trout. Elevated alternative complement activity and oxygen radical production were found after 23 h recovery, and reduced lysozyme activity was found after 46 h, in brown trout only. Results indicate that differences in the stress response of these closely related species, as illustrated by the intensity of the cortisol response, originate at the level of the pituitary and are also manifested through secondary stress responses.