Feasibility of chitosan-alginate (Chi-Alg) hydrogel used as scaffold for neural tissue engineering: a pilot study in vitro

In tissue engineering, scaffolding plays an important role in accommodating and stimulating new tissue growth. Chitosan and alginate are two widely used natural polymers in tissue engineering. Here, we prepared the chitosan-alginate (Chi-Alg) hydrogel from naturally derived chitosan and alginate pol...

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Published inBiotechnology, biotechnological equipment Vol. 31; no. 4; pp. 766 - 773
Main Authors Wang, Gan, Wang, Xiaoyan, Huang, Lixiang
Format Journal Article
LanguageEnglish
Published Sofia Taylor & Francis 04.07.2017
Taylor & Francis Ltd
Taylor & Francis Group
Subjects
alginate
Alginates
Alginic acid
Amino groups
biotechnology
cell proliferation
Chitosan
Contact angle
hydrogel
Hydrogels
hydrophilicity
Infrared spectroscopy
Ionic interactions
Natural polymers
nerve tissue
Nervous tissues
Neural stem cells
Olfactory ensheathing cells
Polymers
Scaffolding
Scanning electron microscopy
Stem cells
Tissue engineering
X-ray diffraction
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Abstract In tissue engineering, scaffolding plays an important role in accommodating and stimulating new tissue growth. Chitosan and alginate are two widely used natural polymers in tissue engineering. Here, we prepared the chitosan-alginate (Chi-Alg) hydrogel from naturally derived chitosan and alginate polymers. The Fourier-transformed infrared spectroscopy and X-ray diffraction results demonstrated that a chitosan-alginate hydrogel was constructed due to the strong ionic interaction between the positively charged amino groups of chitosan and the negatively charged carboxyl groups of alginate. The scanning electron microscopy and contact angle results showed the inner porous structure and highly hydrophilic property of chitosan-alginate hydrogel. As the two most promising cell types in nerve tissue engineering, both olfactory ensheathing cells and neural stem cells proliferated well on the chitosan-alginate hydrogel. All results indicated the good potential application of a chitosan-alginate hydrogel for neural tissue engineering.
AbstractList In tissue engineering, scaffolding plays an important role in accommodating and stimulating new tissue growth. Chitosan and alginate are two widely used natural polymers in tissue engineering. Here, we prepared the chitosan-alginate (Chi-Alg) hydrogel from naturally derived chitosan and alginate polymers. The Fourier-transformed infrared spectroscopy and X-ray diffraction results demonstrated that a chitosan-alginate hydrogel was constructed due to the strong ionic interaction between the positively charged amino groups of chitosan and the negatively charged carboxyl groups of alginate. The scanning electron microscopy and contact angle results showed the inner porous structure and highly hydrophilic property of chitosan-alginate hydrogel. As the two most promising cell types in nerve tissue engineering, both olfactory ensheathing cells and neural stem cells proliferated well on the chitosan-alginate hydrogel. All results indicated the good potential application of a chitosan-alginate hydrogel for neural tissue engineering.
Author Huang, Lixiang
Wang, Gan
Wang, Xiaoyan
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  surname: Huang
  fullname: Huang, Lixiang
  organization: Department of Chemistry and Biology, College of Science, National University of Defense Technology
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Snippet In tissue engineering, scaffolding plays an important role in accommodating and stimulating new tissue growth. Chitosan and alginate are two widely used...
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SubjectTerms alginate
Alginates
Alginic acid
Amino groups
biotechnology
cell proliferation
Chitosan
Contact angle
hydrogel
Hydrogels
hydrophilicity
Infrared spectroscopy
Ionic interactions
Natural polymers
nerve tissue
Nervous tissues
Neural stem cells
Olfactory ensheathing cells
Polymers
Scaffolding
Scanning electron microscopy
Stem cells
Tissue engineering
X-ray diffraction
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Title Feasibility of chitosan-alginate (Chi-Alg) hydrogel used as scaffold for neural tissue engineering: a pilot study in vitro
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