Gonadotropin-Releasing Hormone Stimulates Annexin 5 Messenger Ribonucleic Acid Expression in the Anterior Pituitary Cells

• Published in: Biochemical and biophysical research communications Vol. 291; no. 4; pp. 915 - 920
• Main Authors: Kawaminami, Mitsumori, Tsuchiyama, Yoshiyuki, Saito, Shihori, Katayama, Mikio, Kurusu, Shiro, Hashimoto, Inoru
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 08.03.2002
• Subjects: Animals; annexin 5; Annexin A5 - biosynthesis; Annexin A5 - genetics; Annexin A5 - pharmacology; Cells, Cultured; Colforsin - pharmacology; Female; GnRH; Gonadotropin-Releasing Hormone - pharmacology; Kinetics; LH β subunit; Luteinizing Hormone - biosynthesis; Luteinizing Hormone - genetics; pituitary gland; Pituitary Gland, Anterior - metabolism; protein kinase C; Rats; Rats, Wistar; RNA, Messenger - biosynthesis; semiquantitative RT-PCR; Tetradecanoylphorbol Acetate - pharmacology; Transcriptional Activation; semiquantitative RT-PCR; annexin 5; GnRH; pituitary gland; LH β subunit; protein kinase C
• ISSN: 0006-291X; 1090-2104
• DOI: 10.1006/bbrc.2002.6573

We previously reported that annexin 5 is found specifically in gonadotropes and that the expression is dramatically enhanced after ovariectomy. In the present study, the expression of annexin 5 was examined in the primary culture of rat anterior pituitary cells using semiquantitative RT-PCR to determine if it is under the direct control of gonadotropin-releasing hormone (GnRH). Continuous administration of GnRH analog for 1 h enhanced the expression of both FSH β subunit and annexin 5 mRNA. The expression of annexin 5 mRNA was also augmented by phorbol 12-myristate 13-acetate but not by forskolin. Administration of recombinant rat annexin 5 to the culture increased LH β mRNA expression. These data clearly demonstrate that the expression of annexin 5 mRNA is directly controlled by GnRH and suggest that annexin 5 is involved in mediating GnRH action in the pituitary gland.