The Oncogene Nup98-HOXA9 Induces Gene Transcription in Myeloid Cells

• Published in: The Journal of biological chemistry Vol. 279; no. 2; pp. 866 - 875
• Main Authors: Ghannam, Ghada, Takeda, Akiko, Camarata, Troy, Moore, Malcolm A., Viale, Agnes, Yaseen, Nabeel R.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 09.01.2004; American Society for Biochemistry and Molecular Biology
• Subjects: Animals; Blotting, Western; Cell Cycle; Cell Division; Cell Survival; DNA microarrays; Down-Regulation; Flow Cytometry; Gene Expression Regulation, Neoplastic; Genes, Reporter; Green Fluorescent Proteins; Homeodomain Proteins - physiology; HOXA9 gene; Humans; Image Processing, Computer-Assisted; K562 Cells; Leukemia, Myeloid, Acute - drug therapy; Leukemia, Myeloid, Acute - metabolism; Luciferases - metabolism; Luminescent Proteins - metabolism; Mice; Models, Biological; Myeloid Cells - metabolism; Nuclear Pore Complex Proteins - physiology; nucleoporins; Nup98 gene; Oligonucleotide Array Sequence Analysis; Oncogene Proteins, Fusion - physiology; Plasmids - metabolism; Protein Structure, Tertiary; Retroviridae - genetics; Reverse Transcriptase Polymerase Chain Reaction; Transcription, Genetic; Transcriptional Activation; Transfection; Up-Regulation

The nucleoporin Nup98 gene is frequently rearranged in acute myelogenous leukemia (AML). In most cases this results in fusion of the N terminus of Nup98 to the DNA binding domain of a homeodomain transcription factor. The prototype of these fusions, Nup98-HOXA9, is associated with human AML and indu...