Properties and secretory mechanism of Musca domestica digestive chymotrypsin and its relation with Drosophila melanogaster homologs

• Published in: Insect biochemistry and molecular biology Vol. 42; no. 7; pp. 482 - 490
• Main Authors: Tamaki, Fábio K, Padilha, Marcelo H.P, Pimentel, Andre C, Ribeiro, Alberto F, Terra, Walter R
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 01.07.2012
• Subjects: Amino Acid Sequence; Animals; antiserum; Cell membranes; Chymotrypsin; Chymotrypsin - chemistry; Chymotrypsin - genetics; Chymotrypsin - metabolism; Chymotrypsin expression; Chymotrypsin secretion; Chymotrypsin sequence; Cloning, Molecular; Digestive System - chemistry; Digestive System - enzymology; Dipteran chymotrypsins; DNA, Complementary - genetics; Drosophila melanogaster; Drosophila melanogaster - chemistry; Drosophila melanogaster - enzymology; Drosophila melanogaster - genetics; Enzymes; Escherichia coli; Evolution; Exocytosis; Gene expression; genes; half life; heat inactivation; Houseflies - chemistry; Houseflies - enzymology; Houseflies - genetics; Insect Proteins - chemistry; Insect Proteins - genetics; Insect Proteins - metabolism; Larva - chemistry; Larva - enzymology; Larva - genetics; Larvae; Membrane-bound chymotrypsin; Midgut; Migration; molecular weight; Musca; Musca domestica; pH effects; Phylogeny; polyacrylamide gel electrophoresis; Polymerase chain reaction; Recombinant Proteins - chemistry; Recombinant Proteins - genetics; Recombinant Proteins - metabolism; Reverse Transcriptase Polymerase Chain Reaction; sequence analysis; Sequence Analysis, DNA; Soluble chymotrypsin; transcriptome; Vesicles; Western blotting; Chymotrypsin expression; Chymotrypsin sequence; Chymotrypsin secretion; Membrane-bound chymotrypsin; Dipteran chymotrypsins; Soluble chymotrypsin

Musca domestica larvae present two different digestive chymotryptic activities found in the posterior midgut (PMG): one major soluble activity in the lumen and another minor present in cell membrane fractions. Both soluble and membrane-bound chymotryptic activities have different half lives of therm...