Reduced MiR-675 in Exosome in H19 RNA-Related Melanogenesis via MITF as a Direct Target

• Published in: Journal of investigative dermatology Vol. 134; no. 4; pp. 1075 - 1082
• Main Authors: Kim, Nan-Hyung, Choi, Soo-Hyun, Kim, Chang-Hyun, Lee, Chang Hoon, Lee, Tae Ryong, Lee, Ai-Young
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.04.2014; Elsevier Limited
• Subjects: 3' Untranslated Regions; Animals; Apoptosis; Cell Separation; Epidermis - metabolism; Exosomes - metabolism; Female; Fibroblasts - cytology; Fibroblasts - metabolism; Flow Cytometry; Gene Expression Regulation; Humans; Keratinocytes - cytology; Melanocytes - cytology; Mice; Mice, Inbred C57BL; Microphthalmia-Associated Transcription Factor - metabolism; MicroRNAs - genetics; MicroRNAs - metabolism; Oligonucleotide Array Sequence Analysis; RNA, Long Noncoding - genetics; Tetraspanin 30 - metabolism

H19 non-coding RNA downregulation stimulates melanogenesis in melasma patients. However, its mechanism is unclear. In this study, the potential role of a H19 microRNA, miR-675, in melanogenesis was examined. Real-time PCR using cultured normal human skin keratinocytes, melanocytes, and fibroblasts w...