A Senescent Cluster in Aged Human Hematopoietic Stem Cell Compartment as Target for Senotherapy

• Published in: International journal of molecular sciences Vol. 26; no. 2; p. 787
• Main Authors: Poisa-Beiro, Laura, Landry, Jonathan J. M., Yan, Bowen, Kardorff, Michael, Eckstein, Volker, Villacorta, Laura, Krammer, Peter H., Zaugg, Judith, Gavin, Anne-Claude, Benes, Vladimir, Zhou, Daohong, Raffel, Simon, Ho, Anthony D.
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 17.01.2025; MDPI
• Subjects: Adult; Age groups; Aged; Aging; Aging - genetics; Animals; Bone marrow; Cell cycle; Cellular Senescence - genetics; Comparative analysis; Cyclin-Dependent Kinase Inhibitor p16 - metabolism; DNA damage; DNA methylation; DNA repair; Evolution; Female; Gene Expression Profiling; Genes; Genotype & phenotype; Granulocytes; Hematopoiesis; Hematopoietic Stem Cells - cytology; Hematopoietic Stem Cells - drug effects; Hematopoietic Stem Cells - metabolism; Human subjects; Humans; Male; Metabolism; Mice; Senescence; Senotherapeutics - pharmacology; Stem cells; Telomeres; Transcriptome; Tumor proteins; Tumor Suppressor Protein p53 - metabolism; Germany; senescence signature; hematopoietic stem and progenitor cells (HSPC); aging; comparative single-cell transcriptomics

To identify the differences between aged and young human hematopoiesis, we performed a direct comparison of aged and young human hematopoietic stem and progenitor cells (HSPCs). Alterations in transcriptome profiles upon aging between humans and mice were then compared. Human specimens consist of CD...