New approaches to identification and activity estimation of glyphosate degradation enzymes
We propose a new set of approaches, which allow identifying the primary enzymes of glyphosate (N-phosphonomethyl-glycine) attack, measuring their activities, and quantitative analysis of glyphosate degradation in vivo and in vitro . Using the developed approach we show that glyphosate degradation ca...
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Published in | Biochemistry (Moscow) Vol. 76; no. 6; pp. 720 - 725 |
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Main Authors | , , , , |
Format | Journal Article |
Language | English |
Published |
Dordrecht
SP MAIK Nauka/Interperiodica
01.06.2011
Springer Springer Nature B.V |
Subjects | |
Online Access | Get full text |
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Summary: | We propose a new set of approaches, which allow identifying the primary enzymes of glyphosate (N-phosphonomethyl-glycine) attack, measuring their activities, and quantitative analysis of glyphosate degradation
in vivo
and
in vitro
. Using the developed approach we show that glyphosate degradation can follow different pathways depending on physiological characteristics of metabolizing strains: in
Ochrobactrum anthropi
GPK3 the initial cleavage reaction is catalyzed by glyphosate-oxidoreductase with the formation of aminomethylphosphonic acid and glyoxylate, whereas
Achromobacter
sp. MPS12 utilize C-P lyase, forming sarcosine. The proposed methodology has several advantages as compared to others described in the literature. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 0006-2979 1608-3040 |
DOI: | 10.1134/S0006297911060149 |