Specific and sensitive GC–MS analysis of hypusine, Nε-(4-amino-2-hydroxybutyl)lysine, a biomarker of hypusinated eukaryotic initiation factor eIF5A, and its application to the bi-ethnic ASOS study

• Published in: Amino acids Vol. 54; no. 7; pp. 1083 - 1099
• Main Authors: Baskal, Svetlana, Kaiser, Annette, Mels, Catharina, Kruger, Ruan, Tsikas, Dimitrios
• Format: Journal Article
• Language: English
• Published: Vienna Springer Vienna 01.07.2022; Springer Nature B.V
• Subjects: Acetic acid; Acylation; advanced glycation end products; Advanced glycosylation end products; Amino acids; Analytical Chemistry; anhydrides; Biochemical Engineering; Biochemistry; Biomarkers; Biomedical and Life Sciences; Creatinine; derivatization; detection limit; Deuterium; Esterification; Esters; Ethnic factors; Ethyl acetate; Excretion; females; Gas chromatography; gas chromatography-mass spectrometry; Glycosylation; Initiation factor eIF-5A; Ionization; Life Sciences; Lysine; males; Mass spectrometry; Mass spectroscopy; Neurobiology; Original; Original Article; Post-translation; post-translational modification; progeny; Proteomics; Stiffness; Urine; Deuterium; GC–MS; Quantification; ASOS; Amino acids; AGEs; Derivatization; Post-translational modification (PTM); eIF5A; Hypusine

Hypusination is a unique two-step enzymatic post-translational modification of the N ε -amino group of lysine-50 of the eukaryotic initiation factor 5A (eIF5A). We developed a specific and sensitive gas chromatography–mass spectrometry (GC–MS) method for the measurement of biological hypusine (Hyp),...