Internalization of Ia molecules by antigen-presenting B cells is limited

• Published in: The Journal of immunology (1950) Vol. 145; no. 7; pp. 2064 - 2069
• Main Authors: Mehringer, JH, Cullen, SE
• Format: Journal Article
• Language: English
• Published: Bethesda, MD Am Assoc Immnol 01.10.1990; American Association of Immunologists
• Subjects: Analysis of the immune response. Humoral and cellular immunity; Animals; Antigen-Presenting Cells - physiology; B-Lymphocytes - physiology; Biological and medical sciences; Cell Compartmentation - drug effects; Cells, Cultured; Endocytosis - drug effects; Fundamental and applied biological sciences. Psychology; Fundamental immunology; Histocompatibility Antigens Class II - metabolism; Immunobiology; Immunoglobulin Fab Fragments - metabolism; In Vitro Techniques; Lymphocyte Activation; Mice; Monensin - pharmacology; Organs and cells involved in the immune response; Receptors, Fc - physiology; Spleen - cytology; Time Factors; Transferrin - metabolism; Antigen; Antigen presentation; Vertebrata; Mammalia; Mouse; Major histocompatibility system; Internalization; Rodentia; Class II histocompatibility antigen; Accessory cell; B-Lymphocyte; H-2-System

Our data demonstrate that the uptake of surface Ia into an intracellular compartment of B lymphoma or normal spleen cells is limited to about 20% after 2 to 3 h. The extent of internalization does not vary with several types of stimulation, including LPS, phorbol esters, anti-Ig-plus phorbol ester-s...