Transcription of cauliflower mosaic virus integrated into plant genomes

• Published in: Virology (New York, N.Y.) Vol. 140; no. 2; pp. 281 - 288
• Main Authors: Shewmaker, C.K., Caton, John R., Houck, Catherine M., Gardner, Richard C.
• Format: Journal Article
• Language: English
• Published: San Diego, CA Elsevier Inc 30.01.1985; Elsevier
• Subjects: Biological and medical sciences; Biology of microorganisms of confirmed or potential industrial interest; Biotechnology; CAULIFLOWER MOSAIC VIRUS; Fundamental and applied biological sciences. Psychology; Genetics; GENOMAS; GENOME; GENOMES; Microbiology; Mission oriented research; Phytopathology. Animal pests. Plant and forest protection; PLANT VIRUSES; Plant viruses and viroids; PLANTAS; PLANTE; PLANTS; Replicative cycle, interference, host-virus relations, pathogenicity, miscellaneous strains; Systematics. Structure, properties and multiplication. Genetics; Virology; VIRUS DE LAS PLANTAS; VIRUS DES VEGETAUX; Crown gall; Molecular hybridization; Vegetals; Cauliflower mosaic virus; Transcription; Phytopathogen; Gene expression; Virus; Agrobacterium; Caulimovirus; Ti Plasmid; Bacteria; Rhizobiaceae; Molecular cloning; Genome
• ISSN: 0042-6822; 1096-0341
• DOI: 10.1016/0042-6822(85)90366-6

A full-length copy of cauliflower mosaic virus (CaMV) was introduced into the T DNA of the Agrobacterium Ti plasmid and integrated into plant genomes. Northern analysis of turnip galls revealed two transcripts derived from the integrated CaMV. A 1900-nucleotide (1.9 kb) transcript arises from the region VI area of the genome and is the same size as the region VI transcript found in CaMV-infected leaves. A larger 7.5-kb transcript initiates at or near the other known CaMV promoter, which in infected cells directs synthesis of a full-length transcript, and terminates in adjacent T-DNA sequences. Comparison of the two transcripts in galls from seven plant species showed wide variation of both the ratios of the two transcripts within one species and of their levels between species.