Kinetic and Thermodynamic Analysis of Mutant Type II DNA Topoisomerases That Cannot Covalently Cleave DNA

• Published in: The Journal of biological chemistry Vol. 274; no. 6; pp. 3446 - 3452
• Main Authors: Morris, Shayne K., Harkins, Timothy T., Tennyson, Rachel B., Lindsley, Janet E.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 05.02.1999; American Society for Biochemistry and Molecular Biology
• Subjects: Adenosine Triphosphatases - metabolism; Base Sequence; DNA - metabolism; DNA Primers; DNA Topoisomerases, Type II - chemistry; DNA Topoisomerases, Type II - metabolism; Hydrolysis; Kinetics; Saccharomyces cerevisiae; Thermodynamics

DNA topoisomerase II catalyzes two different chemical reactions as part of its DNA transport cycle: ATP hydrolysis and DNA breakage/religation. The coordination between these reactions was studied using mutants of yeast topoisomerase II that are unable to covalently cleave DNA. In the absence of DNA...