Capreomycin Binds across the Ribosomal Subunit Interface Using tlyA-Encoded 2′-O-Methylations in 16S and 23S rRNAs

• Published in: Molecular cell Vol. 23; no. 2; pp. 173 - 182
• Main Authors: Johansen, Shanna K., Maus, Courtney E., Plikaytis, Bonnie B., Douthwaite, Stephen
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 21.07.2006
• Subjects: Antibiotics, Antitubercular - pharmacology; Bacterial Proteins - genetics; Bacterial Proteins - metabolism; Base Sequence; Capreomycin - pharmacology; Cloning, Molecular; Drug Resistance, Bacterial - genetics; Escherichia coli; Escherichia coli - drug effects; Escherichia coli - genetics; Escherichia coli - metabolism; Mass Spectrometry; Methylation; MICROBIO; Molecular Sequence Data; Mutation; Mycobacteriaceae - drug effects; Mycobacterium tuberculosis; Nucleic Acid Conformation; Recombinant Proteins - genetics; Recombinant Proteins - metabolism; Ribosomes - genetics; Ribosomes - metabolism; RNA; RNA, Bacterial - drug effects; RNA, Bacterial - genetics; RNA, Bacterial - metabolism; RNA, Ribosomal, 16S - genetics; RNA, Ribosomal, 16S - metabolism; RNA, Ribosomal, 28S - genetics; RNA, Ribosomal, 28S - metabolism; RNA; MICROBIO

The cyclic peptide antibiotics capreomycin and viomycin are generally effective against the bacterial pathogen Mycobacterium tuberculosis. However, recent virulent isolates have become resistant by inactivation of their tlyA gene. We show here that tlyA encodes a 2′-O-methyltransferase that modifies...