Urinary exosomal long noncoding RNAs serve as biomarkers for early detection of non-small cell lung cancer

• Published in: Bioscience reports Vol. 41; no. 10; p. 1
• Main Authors: Lin, Quan, Xie, Danli, Pan, Liangliang, Lou, Yongliang, Shi, Mengru
• Format: Journal Article
• Language: English
• Published: England Portland Press Ltd The Biochemical Society 01.10.2021; Portland Press Ltd
• Subjects: 1-Phosphatidylinositol 3-kinase; Aged; AKT protein; Apoptosis; Biological activity; Biomarkers; Biomarkers, Tumor - genetics; Biomarkers, Tumor - urine; Biosynthesis; Cancer; Cancer therapies; Carcinoma, Non-Small-Cell Lung - genetics; Carcinoma, Non-Small-Cell Lung - pathology; Carcinoma, Non-Small-Cell Lung - urine; Case-Control Studies; Cell cycle; Cell proliferation; Databases, Genetic; Diagnostics & Biomarkers; DNA microarrays; Early Detection of Cancer; Encyclopedias; Exosomes; Exosomes - genetics; Exosomes - metabolism; Female; Forkhead protein; Gene Expression Regulation, Neoplastic; Genomes; Humans; Lung cancer; Lung Neoplasms - genetics; Lung Neoplasms - pathology; Lung Neoplasms - urine; Male; Medical prognosis; Membranes; MicroRNAs; Middle Aged; Nanoparticles; Non-coding RNA; Non-small cell lung carcinoma; p53 Protein; Pathogenesis; Predictive Value of Tests; Proteins; RNA polymerase; RNA, Long Noncoding - genetics; RNA, Long Noncoding - urine; Sediments; Small cell lung carcinoma; Survival; Transmission electron microscopy; Tumors; Urinalysis; Urine; lncRNA; NSCLC; biomarker; urine; exosome
• ISSN: 0144-8463; 1573-4935
• DOI: 10.1042/BSR20210908

Increasing the efficiency of early diagnosis using noninvasive biomarkers is crucial for enhancing the survival rate of lung cancer patients. We explore the differential expression of non-small cell lung cancer (NSCLC)-related long noncoding RNAs (lncRNAs) in urinary exosomes in NSCLC patients and normal controls to diagnose lung cancer. A differential expression analysis between NSCLC patients and healthy controls was performed using microarrays. Gene ontology (GO) term and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses were used to predict potential functions of lncRNAs in NSCLC. quantitative real-time PCR (QT-PCR) was used to verify microarray results. A total of 640 lncRNAs (70 up- and 570 down-regulated) were differentially expressed in NSCLC patients in comparison to healthy controls. Six lncRNAs were detected by QT-PCR. GO term and KEGG pathway analyses showed that differential lncRNAs were enriched in cellular component organization or biogenesis, as well as other biological processes and signaling pathways, such as the PI3K-AKT, FOXO, p53, and fatty acid biosynthesis. The differential lncRNAs in urinary exosomes are potential diagnostic biomarkers of NSCLC. The lncRNAs enriched in specific pathways may be associated with tumor cell proliferation, tumor cell apoptosis, and the cell cycle involved in the pathogenesis of NSCLC.