Phylogeny of the seal mycoplasmas Mycoplasma phocae corrig., Mycoplasma phocicerebrale corrig. and Mycoplasma phocirhinis corrig. based on sequence analysis of 16S rDNA

• Published in: International journal of systematic and evolutionary microbiology Vol. 51; no. 4; pp. 1389 - 1393
• Main Authors: Heldtander Konigsson, M, Pettersson, B, Johansson, KE
• Format: Journal Article
• Language: English
• Published: Reading Soc General Microbiol 01.07.2001; Society for General Microbiology
• Subjects: Animals; Bacteriology; Biological and medical sciences; DNA, Bacterial - genetics; DNA, Ribosomal - genetics; Fundamental and applied biological sciences. Psychology; Genes, Bacterial; Genetics; Microbiology; Molecular Sequence Data; Mycoplasma - classification; Mycoplasma - genetics; Mycoplasma - isolation & purification; Mycoplasma - metabolism; Mycoplasma alkalescens; Mycoplasma bovis; Mycoplasma hominis; Mycoplasma phocae; Mycoplasma phocicerebrale; Mycoplasma phocirhinis; Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains; Phylogeny; Polymorphism, Genetic; RNA, Bacterial - genetics; RNA, Ribosomal, 16S - genetics; rRNA 16S; Seals, Earless - microbiology; Serotyping; Chromosomal aberration; Adenosine; Mycoplasma hominis; Purine nucleoside; Nucleotide sequence; Ribosomal DNA; Phylogeny; Mycoplasma bovis; Mycoplasmataceae; Cycle; Strain; Mollicutes; Mycoplasmatales; Type; Gene; Ribosomal RNA; Nucleotide; 16S-RNA; Bacteria; Abnormal chromosome; Mycoplasma bovigenitalium; Sequencing; Polymorphism
• DOI: 10.1099/00207713-51-4-1389
• ISSN: 1466-5026

M Heldtander Konigsson, B Pettersson and KE Johansson Department of Bacteriology, National Veterinary Institute, SE-751 89 Uppsala, Sweden The nucleotide sequences of the 16S rRNA genes from the type strains of three seal mycoplasmas, Mycoplasma phocicerebrale, Mycoplasma phocae and Mycoplasma phocirhinis (formerly Mycoplasma phocacerebrale, Mycoplasma phocidae and Mycoplasma phocarhinis, respectively), were determined by direct DNA cycle sequencing. Polymorphisms were found in all three 16S rRNA gene sequences, showing the existence of two different rRNA operons. In M. phocae, a length difference was found between the operons, caused by an insertion or a deletion of an adenosine in one of the operons. The sequence information was used to construct phylogenetic trees. All three species were found to belong to the hominis group, but to different clusters. M. phocicerebrale and M. phocae were found to be members of the Mycoplasma hominis cluster, within which M. phocicerebrale grouped in the Mycoplasma alkalescens subcluster. M. phocirhinis was found to be a member of the Mycoplasma bovigenitalium subcluster of the Mycoplasma bovis cluster. The 16S rRNA gene sequences of all hitherto validly described species within the M. hominis and M. bovis clusters have now been determined.