Identification and characterization of a novel freezing inducible gene, li16, in the wood frog Rana sylvatica

• Published in: The FASEB journal Vol. 16; no. 8; p. 902
• Main Authors: McNally, J Dayre, Wu, Shao-Bo, Sturgeon, Christopher M, Storey, Kenneth B
• Format: Journal Article
• Language: English
• Published: United States 01.06.2002
• Subjects: Amphibian Proteins - genetics; Amphibian Proteins - metabolism; Animals; Blotting, Northern; Blotting, Western; Dibutyryl Cyclic GMP - pharmacology; Dose-Response Relationship, Drug; Freezing; Gene Expression Regulation - drug effects; Hypoxia - physiopathology; Intestines - metabolism; Liver - metabolism; Myocardium - metabolism; Ranidae - genetics; RNA, Messenger - genetics; RNA, Messenger - metabolism; Temperature; Time Factors; Transcription, Genetic; Up-Regulation - drug effects; Water Deprivation - physiology

The wood frog Rana sylvatica survives for weeks during winter hibernation with up to 65% body water frozen as ice. Natural freeze tolerance includes both seasonal and freeze-induced molecular adaptations that control ice formation, deal with long-term ischemia, regulate cell volume changes, and prot...