Identification of beta-carotene 15, 15'-monooxygenase as a peroxisome proliferator-activated receptor target gene

• Published in: The FASEB journal Vol. 17; no. 10; p. 1304
• Main Authors: Boulanger, Ana, McLemore, Pamela, Copeland, Neal G, Gilbert, Debra J, Jenkins, Nancy A, Yu, Shirley S, Gentleman, Susan, Redmond, T Michael
• Format: Journal Article
• Language: English
• Published: United States 01.07.2003
• Subjects: Animals; beta-Carotene 15,15'-Monooxygenase; Binding Sites; Caco-2 Cells; Cell Line; Cloning, Molecular; Humans; Mice; Models, Genetic; Oxygenases - genetics; Promoter Regions, Genetic; Receptors, Cytoplasmic and Nuclear - agonists; Receptors, Cytoplasmic and Nuclear - metabolism; Response Elements; Transcription Factors - agonists; Transcription Factors - metabolism; Transcriptional Activation

Beta-carotene 15,15'-monooxygenase (BCM) catalyzes the first step of vitamin A biosynthesis from provitamin A carotenoids. We wished to determine the factors underlying the transcriptional regulation of this gene. After cloning of the 40 kilobase pair (kbp) mouse Bcm gene and determination of i...