Cell culture systems for isolation of SARS-CoV-2 clinical isolates and generation of recombinant virus

• Published in: iScience Vol. 26; no. 5; p. 106634
• Main Authors: Chen, Da-Yuan, Turcinovic, Jacquelyn, Feng, Shuchen, Kenney, Devin J., Chin, Chue Vin, Choudhary, Manish C., Conway, Hasahn L., Semaan, Marc, Close, Brianna J., Tavares, Alexander H., Seitz, Scott, Khan, Nazimuddin, Kapell, Sebastian, Crossland, Nicholas A., Li, Jonathan Z., Douam, Florian, Baker, Susan C., Connor, John H., Saeed, Mohsan
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 19.05.2023; The Author(s); Elsevier
• Subjects: Methodology in biological sciences; Virology; Methodology in biological sciences; Virology

A simple and robust cell culture system is essential for generating authentic SARS-CoV-2 stocks for evaluation of viral pathogenicity, screening of antiviral compounds, and preparation of inactivated vaccines. Evidence suggests that Vero E6, a cell line commonly used in the field to grow SARS-CoV-2,...