Cell culture systems for isolation of SARS-CoV-2 clinical isolates and generation of recombinant virus

A simple and robust cell culture system is essential for generating authentic SARS-CoV-2 stocks for evaluation of viral pathogenicity, screening of antiviral compounds, and preparation of inactivated vaccines. Evidence suggests that Vero E6, a cell line commonly used in the field to grow SARS-CoV-2,...

Full description

Saved in:
Bibliographic Details
Published iniScience Vol. 26; no. 5; p. 106634
Main Authors Chen, Da-Yuan, Turcinovic, Jacquelyn, Feng, Shuchen, Kenney, Devin J., Chin, Chue Vin, Choudhary, Manish C., Conway, Hasahn L., Semaan, Marc, Close, Brianna J., Tavares, Alexander H., Seitz, Scott, Khan, Nazimuddin, Kapell, Sebastian, Crossland, Nicholas A., Li, Jonathan Z., Douam, Florian, Baker, Susan C., Connor, John H., Saeed, Mohsan
Format Journal Article
LanguageEnglish
Published United States Elsevier Inc 19.05.2023
The Author(s)
Elsevier
Subjects
Methodology in biological sciences
Virology
Methodology in biological sciences
Virology
Online AccessGet full text

Cover

Abstract A simple and robust cell culture system is essential for generating authentic SARS-CoV-2 stocks for evaluation of viral pathogenicity, screening of antiviral compounds, and preparation of inactivated vaccines. Evidence suggests that Vero E6, a cell line commonly used in the field to grow SARS-CoV-2, does not support efficient propagation of new viral variants and triggers rapid cell culture adaptation of the virus. We generated a panel of 17 human cell lines overexpressing SARS-CoV-2 entry factors and tested their ability to support viral infection. Two cell lines, Caco-2/AT and HuH-6/AT, demonstrated exceptional susceptibility, yielding highly concentrated virus stocks. Notably, these cell lines were more sensitive than Vero E6 cells in recovering SARS-CoV-2 from clinical specimens. Further, Caco-2/AT cells provided a robust platform for producing genetically reliable recombinant SARS-CoV-2 through a reverse genetics system. These cellular models are a valuable tool for the study of SARS-CoV-2 and its continuously emerging variants. [Display omitted] •Human cell lines overexpressing ACE2 and TMPRSS2 were tested for SARS-CoV-2 infection•Two cell lines, Caco-2 and HuH-6, exhibited remarkable susceptibility to infection•Caco-2 and HuH-6 cells allowed isolation of SARS-CoV-2 from clinical specimens•Caco-2 cells supported production of genetically stable recombinant SARS-CoV-2 Methodology in biological sciences; Virology
AbstractList A simple and robust cell culture system is essential for generating authentic SARS-CoV-2 stocks for evaluation of viral pathogenicity, screening of antiviral compounds, and preparation of inactivated vaccines. Evidence suggests that Vero E6, a cell line commonly used in the field to grow SARS-CoV-2, does not support efficient propagation of new viral variants and triggers rapid cell culture adaptation of the virus. We generated a panel of 17 human cell lines overexpressing SARS-CoV-2 entry factors and tested their ability to support viral infection. Two cell lines, Caco-2/AT and HuH-6/AT, demonstrated exceptional susceptibility, yielding highly concentrated virus stocks. Notably, these cell lines were more sensitive than Vero E6 cells in recovering SARS-CoV-2 from clinical specimens. Further, Caco-2/AT cells provided a robust platform for producing genetically reliable recombinant SARS-CoV-2 through a reverse genetics system. These cellular models are a valuable tool for the study of SARS-CoV-2 and its continuously emerging variants.A simple and robust cell culture system is essential for generating authentic SARS-CoV-2 stocks for evaluation of viral pathogenicity, screening of antiviral compounds, and preparation of inactivated vaccines. Evidence suggests that Vero E6, a cell line commonly used in the field to grow SARS-CoV-2, does not support efficient propagation of new viral variants and triggers rapid cell culture adaptation of the virus. We generated a panel of 17 human cell lines overexpressing SARS-CoV-2 entry factors and tested their ability to support viral infection. Two cell lines, Caco-2/AT and HuH-6/AT, demonstrated exceptional susceptibility, yielding highly concentrated virus stocks. Notably, these cell lines were more sensitive than Vero E6 cells in recovering SARS-CoV-2 from clinical specimens. Further, Caco-2/AT cells provided a robust platform for producing genetically reliable recombinant SARS-CoV-2 through a reverse genetics system. These cellular models are a valuable tool for the study of SARS-CoV-2 and its continuously emerging variants.
A simple and robust cell culture system is essential for generating authentic SARS-CoV-2 stocks for evaluation of viral pathogenicity, screening of antiviral compounds, and preparation of inactivated vaccines. Evidence suggests that Vero E6, a cell line commonly used in the field to grow SARS-CoV-2, does not support efficient propagation of new viral variants and triggers rapid cell culture adaptation of the virus. We generated a panel of 17 human cell lines overexpressing SARS-CoV-2 entry factors and tested their ability to support viral infection. Two cell lines, Caco-2/AT and HuH-6/AT, demonstrated exceptional susceptibility, yielding highly concentrated virus stocks. Notably, these cell lines were more sensitive than Vero E6 cells in recovering SARS-CoV-2 from clinical specimens. Further, Caco-2/AT cells provided a robust platform for producing genetically reliable recombinant SARS-CoV-2 through a reverse genetics system. These cellular models are a valuable tool for the study of SARS-CoV-2 and its continuously emerging variants.
A simple and robust cell culture system is essential for generating authentic SARS-CoV-2 stocks required for evaluation of viral pathogenicity, screening of antiviral compounds, and preparation of inactivated vaccines. Evidence suggests that Vero E6, a cell line commonly used in the field to grow SARS-CoV-2, does not support efficient propagation of new viral variants and triggers rapid cell culture adaptation of the virus. We generated a panel of 17 human cell lines overexpressing SARS-CoV-2 entry factors and tested their ability to support viral infection. Two cell lines, Caco-2/AT and HuH-6/AT, demonstrated exceptional susceptibility, yielding highly concentrated virus stocks. Notably, these cell lines were more sensitive than Vero E6 cells in recovering SARS-CoV-2 from clinical specimens. Further, Caco-2/AT cells provided a robust platform for producing genetically reliable recombinant SARS-CoV-2 through a reverse genetics system. These cellular models are a valuable tool for the study of SARS-CoV-2 and its continuously emerging variants.
A simple and robust cell culture system is essential for generating authentic SARS-CoV-2 stocks for evaluation of viral pathogenicity, screening of antiviral compounds, and preparation of inactivated vaccines. Evidence suggests that Vero E6, a cell line commonly used in the field to grow SARS-CoV-2, does not support efficient propagation of new viral variants and triggers rapid cell culture adaptation of the virus. We generated a panel of 17 human cell lines overexpressing SARS-CoV-2 entry factors and tested their ability to support viral infection. Two cell lines, Caco-2/AT and HuH-6/AT, demonstrated exceptional susceptibility, yielding highly concentrated virus stocks. Notably, these cell lines were more sensitive than Vero E6 cells in recovering SARS-CoV-2 from clinical specimens. Further, Caco-2/AT cells provided a robust platform for producing genetically reliable recombinant SARS-CoV-2 through a reverse genetics system. These cellular models are a valuable tool for the study of SARS-CoV-2 and its continuously emerging variants. [Display omitted] •Human cell lines overexpressing ACE2 and TMPRSS2 were tested for SARS-CoV-2 infection•Two cell lines, Caco-2 and HuH-6, exhibited remarkable susceptibility to infection•Caco-2 and HuH-6 cells allowed isolation of SARS-CoV-2 from clinical specimens•Caco-2 cells supported production of genetically stable recombinant SARS-CoV-2 Methodology in biological sciences; Virology
ArticleNumber 106634
Author Baker, Susan C.
Feng, Shuchen
Tavares, Alexander H.
Saeed, Mohsan
Crossland, Nicholas A.
Choudhary, Manish C.
Conway, Hasahn L.
Turcinovic, Jacquelyn
Khan, Nazimuddin
Chen, Da-Yuan
Semaan, Marc
Kenney, Devin J.
Kapell, Sebastian
Douam, Florian
Close, Brianna J.
Connor, John H.
Chin, Chue Vin
Li, Jonathan Z.
Seitz, Scott
Author_xml – sequence: 1
  givenname: Da-Yuan
  surname: Chen
  fullname: Chen, Da-Yuan
  organization: Department of Biochemistry, Boston University Chobanian and Avedisian School of Medicine, Boston, MA, USA
– sequence: 2
  givenname: Jacquelyn
  surname: Turcinovic
  fullname: Turcinovic, Jacquelyn
  organization: National Emerging Infectious Diseases Laboratories, Boston University, Boston, MA, USA
– sequence: 3
  givenname: Shuchen
  surname: Feng
  fullname: Feng, Shuchen
  organization: Department of Microbiology and Immunology, Loyola University Chicago Stritch School of Medicine, Maywood, IL, USA
– sequence: 4
  givenname: Devin J.
  surname: Kenney
  fullname: Kenney, Devin J.
  organization: National Emerging Infectious Diseases Laboratories, Boston University, Boston, MA, USA
– sequence: 5
  givenname: Chue Vin
  surname: Chin
  fullname: Chin, Chue Vin
  organization: Department of Biochemistry, Boston University Chobanian and Avedisian School of Medicine, Boston, MA, USA
– sequence: 6
  givenname: Manish C.
  surname: Choudhary
  fullname: Choudhary, Manish C.
  organization: Brigham and Women’s Hospital, Boston, MA, USA
– sequence: 7
  givenname: Hasahn L.
  surname: Conway
  fullname: Conway, Hasahn L.
  organization: Department of Biochemistry, Boston University Chobanian and Avedisian School of Medicine, Boston, MA, USA
– sequence: 8
  givenname: Marc
  surname: Semaan
  fullname: Semaan, Marc
  organization: Department of Biochemistry, Boston University Chobanian and Avedisian School of Medicine, Boston, MA, USA
– sequence: 9
  givenname: Brianna J.
  surname: Close
  fullname: Close, Brianna J.
  organization: National Emerging Infectious Diseases Laboratories, Boston University, Boston, MA, USA
– sequence: 10
  givenname: Alexander H.
  surname: Tavares
  fullname: Tavares, Alexander H.
  organization: Department of Biochemistry, Boston University Chobanian and Avedisian School of Medicine, Boston, MA, USA
– sequence: 11
  givenname: Scott
  surname: Seitz
  fullname: Seitz, Scott
  organization: National Emerging Infectious Diseases Laboratories, Boston University, Boston, MA, USA
– sequence: 12
  givenname: Nazimuddin
  surname: Khan
  fullname: Khan, Nazimuddin
  organization: Department of Biochemistry, Boston University Chobanian and Avedisian School of Medicine, Boston, MA, USA
– sequence: 13
  givenname: Sebastian
  surname: Kapell
  fullname: Kapell, Sebastian
  organization: Department of Biochemistry, Boston University Chobanian and Avedisian School of Medicine, Boston, MA, USA
– sequence: 14
  givenname: Nicholas A.
  surname: Crossland
  fullname: Crossland, Nicholas A.
  organization: National Emerging Infectious Diseases Laboratories, Boston University, Boston, MA, USA
– sequence: 15
  givenname: Jonathan Z.
  surname: Li
  fullname: Li, Jonathan Z.
  organization: Brigham and Women’s Hospital, Boston, MA, USA
– sequence: 16
  givenname: Florian
  surname: Douam
  fullname: Douam, Florian
  organization: National Emerging Infectious Diseases Laboratories, Boston University, Boston, MA, USA
– sequence: 17
  givenname: Susan C.
  surname: Baker
  fullname: Baker, Susan C.
  organization: Department of Microbiology and Immunology, Loyola University Chicago Stritch School of Medicine, Maywood, IL, USA
– sequence: 18
  givenname: John H.
  surname: Connor
  fullname: Connor, John H.
  organization: National Emerging Infectious Diseases Laboratories, Boston University, Boston, MA, USA
– sequence: 19
  givenname: Mohsan
  orcidid: 0000-0001-8505-7054
  surname: Saeed
  fullname: Saeed, Mohsan
  email: msaeed1@bu.edu
  organization: Department of Biochemistry, Boston University Chobanian and Avedisian School of Medicine, Boston, MA, USA
BackLink https://www.ncbi.nlm.nih.gov/pubmed/37095858$$D View this record in MEDLINE/PubMed
BookMark eNp9UltrFDEYHaRiL_YP-CB59GXW3CbJgCBlUVsoCFZ9DdnkmzVLNqnJzEL_vdnOtrQ-FAIJX84FvnNOm6OYIjTNO4IXBBPxcbPwxfoFxZTVgRCMv2pOaKf6FmNOj568j5vzUjYYY1oP78Wb5phJ3HeqUyfNsIQQkJ3COGVA5a6MsC1oSBn5koIZfYooDejm4sdNu0y_W4ps8NFbEw4AKMhEh9YQIT_CM9i0Xflo4oh2Pk_lbfN6MKHA-eE-a359_fJzedlef_92tby4bi1nkrdCcEwclRI4W_FOWiEs44NxQHrOmaCUdNAxTlyHDbPMGGex6q0UypCVxOysuZp1XTIbfZv91uQ7nYzX94OU19rk0dsAmsPQEQqOqg44odKA4lVLcWbAOTlUrc-z1u202oKzEMdswjPR5z_R_9HrtNMEY8UIJ1Xhw0Ehp78TlFFva2h14SZCmoqmCgssSS9phb5_avbo8pBUBagZYHMqJcOgrR_vF169faimet8LvdH7Xuh9L_Tci0ql_1Ef1F8kfZpJUOPaeci6IiBacL6mO9Z9-pfo_wCllNBp
CitedBy_id crossref_primary_10_1021_acs_jmedchem_4c01872
crossref_primary_10_1038_s41598_024_75038_4
crossref_primary_10_3390_v17010037
crossref_primary_10_1126_science_adm9724
Cites_doi 10.1038/s41586-022-04462-1
10.1128/JVI.00862-21
10.1038/s41564-021-00908-w
10.1056/NEJMoa1211721
10.1038/s41586-020-2294-9
10.1016/j.cell.2021.03.052
10.1007/s10096-020-04106-0
10.1371/journal.pbio.3001006
10.3390/v14071408
10.3390/v13122434
10.1126/science.abg3055
10.1038/s41586-021-04387-1
10.1016/j.cell.2020.02.052
10.1080/22221751.2020.1756700
10.1186/s13073-020-00763-0
10.2807/1560-7917.ES.2020.25.3.2000045
10.1016/j.celrep.2021.109364
10.1038/s41586-021-03237-4
10.1056/NEJMoa030747
10.1099/jgv.0.001453
10.3390/ijms221910188
10.1007/s11095-013-1069-5
10.1128/JVI.02422-20
10.1016/S1473-3099(20)30120-1
10.7554/eLife.66815
10.1038/s41586-020-2895-3
10.1038/s41598-021-84882-7
10.1016/j.cmi.2021.05.022
10.1038/s41586-020-2012-7
10.1126/science.abe8499
10.1016/j.celrep.2021.109014
10.1038/s41467-021-23779-5
10.1371/journal.pone.0255622
10.1128/JVI.00790-20
10.1128/mBio.02168-20
10.1038/s41586-023-05697-2
10.7554/eLife.69496
ContentType Journal Article
Copyright 2023 The Author(s)
2023 The Author(s).
2023 The Author(s) 2023
Copyright_xml – notice: 2023 The Author(s)
– notice: 2023 The Author(s).
– notice: 2023 The Author(s) 2023
DBID 6I.
AAFTH
AAYXX
CITATION
NPM
7X8
5PM
DOA
DOI 10.1016/j.isci.2023.106634
DatabaseName ScienceDirect Open Access Titles
Elsevier:ScienceDirect:Open Access
CrossRef
PubMed
MEDLINE - Academic
PubMed Central (Full Participant titles)
Directory of Open Access Journals - May need to register for free articles
DatabaseTitle CrossRef
PubMed
MEDLINE - Academic
DatabaseTitleList MEDLINE - Academic
PubMed
Database_xml – sequence: 1
  dbid: DOA
  name: DOAJ Directory of Open Access Journals
  url: https://www.doaj.org/
  sourceTypes: Open Website
– sequence: 2
  dbid: NPM
  name: PubMed
  url: https://proxy.k.utb.cz/login?url=http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed
  sourceTypes: Index Database
DeliveryMethod fulltext_linktorsrc
EISSN 2589-0042
ExternalDocumentID oai_doaj_org_article_4ef512ed285e4127ae84089843aedd7f
PMC10083141
37095858
10_1016_j_isci_2023_106634
S2589004223007113
Genre Journal Article
GrantInformation_xml – fundername: NIAID NIH HHS
  grantid: R01 AI159945
GroupedDBID 0R~
53G
6I.
AACTN
AAEDW
AAFTH
AALRI
AAMRU
AAXUO
ABMAC
ADBBV
ADVLN
AEXQZ
AFTJW
AITUG
ALMA_UNASSIGNED_HOLDINGS
AMRAJ
AOIJS
BCNDV
EBS
FDB
GROUPED_DOAJ
HYE
M41
OK1
ROL
RPM
SSZ
AAYWO
AAYXX
ACVFH
ADCNI
AEUPX
AFPUW
AIGII
AKBMS
AKYEP
APXCP
CITATION
EJD
NCXOZ
NPM
7X8
5PM
ID FETCH-LOGICAL-c4374-66401d277e43b457c66c34fade1944362215e5341d50a3c3aadc089c768a1b703
IEDL.DBID DOA
ISSN 2589-0042
IngestDate Wed Aug 27 01:31:43 EDT 2025
Thu Aug 21 18:38:05 EDT 2025
Fri Jul 11 02:05:49 EDT 2025
Thu Jan 02 22:39:22 EST 2025
Tue Jul 01 05:31:17 EDT 2025
Thu Apr 24 22:52:14 EDT 2025
Sat Mar 01 15:45:58 EST 2025
IsDoiOpenAccess true
IsOpenAccess true
IsPeerReviewed true
IsScholarly true
Issue 5
Keywords Methodology in biological sciences
Virology
Language English
License This is an open access article under the CC BY-NC-ND license.
2023 The Author(s).
Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.
LinkModel DirectLink
MergedId FETCHMERGED-LOGICAL-c4374-66401d277e43b457c66c34fade1944362215e5341d50a3c3aadc089c768a1b703
Notes ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
Lead contact
ORCID 0000-0001-8505-7054
OpenAccessLink https://doaj.org/article/4ef512ed285e4127ae84089843aedd7f
PMID 37095858
PQID 2806071972
PQPubID 23479
ParticipantIDs doaj_primary_oai_doaj_org_article_4ef512ed285e4127ae84089843aedd7f
pubmedcentral_primary_oai_pubmedcentral_nih_gov_10083141
proquest_miscellaneous_2806071972
pubmed_primary_37095858
crossref_citationtrail_10_1016_j_isci_2023_106634
crossref_primary_10_1016_j_isci_2023_106634
elsevier_sciencedirect_doi_10_1016_j_isci_2023_106634
ProviderPackageCode CITATION
AAYXX
PublicationCentury 2000
PublicationDate 2023-05-19
PublicationDateYYYYMMDD 2023-05-19
PublicationDate_xml – month: 05
  year: 2023
  text: 2023-05-19
  day: 19
PublicationDecade 2020
PublicationPlace United States
PublicationPlace_xml – name: United States
PublicationTitle iScience
PublicationTitleAlternate iScience
PublicationYear 2023
Publisher Elsevier Inc
The Author(s)
Elsevier
Publisher_xml – name: Elsevier Inc
– name: The Author(s)
– name: Elsevier
References Peacock, Goldhill, Zhou, Baillon, Frise, Swann, Kugathasan, Penn, Brown, Sanchez-David (bib20) 2021; 6
Amarilla, Sng, Parry, Deerain, Potter, Setoh, Rawle, Le, Modhiran, Wang (bib14) 2021; 12
Liu, Zheng, Lin, Li, Yuan, Peng, Xiong, Sun, Li, Wu (bib18) 2020; 94
Thi Nhu Thao, Labroussaa, Ebert, V'Kovski, Stalder, Portmann, Kelly, Steiner, Holwerda, Kratzel (bib31) 2020; 582
Suzuki, Yamasoba, Kimura, Wang, Kishimoto, Ito, Morioka, Nao, Nasser, Uriu (bib12) 2022; 603
Feng, Ali, Evdokimova, Reid, Clark, Uprichard, Baker (bib32) 2022; 14
Herrmann, Jungnickl, Cordsmeier, Peter, Überla, Ensser (bib29) 2021; 22
Baczenas, Andersen, Rashid, Yarmosh, Puthuveetil, Parker, Bradford, Florence, Stemple, Lewis, O'Connor (bib33) 2021; 13
Lau, Wang, Mok, Zhang, Chu, Lee, Deng, Chen, Chan, Song (bib17) 2020; 9
Drosten, Günther, Preiser, van der Werf, Brodt, Becker, Rabenau, Panning, Kolesnikova, Fouchier (bib9) 2003; 348
Davies, Abbott, Barnard, Jarvis, Kucharski, Munday, Pearson, Russell, Tully, Washburne (bib4) 2021; 372
Chen, Khan, Close, Goel, Blum, Tavares, Kenney, Conway, Ewoldt, Chitalia (bib34) 2021; 95
Torii, Ono, Suzuki, Morioka, Anzai, Fauzyah, Maeda, Kamitani, Fukuhara, Matsuura (bib13) 2021; 35
Davidson, Williamson, Lewis, Shoemark, Carroll, Heesom, Zambon, Ellis, Lewis, Hiscox, Matthews (bib16) 2020; 12
Johnson, Xie, Bailey, Kalveram, Lokugamage, Muruato, Zou, Zhang, Juelich, Smith (bib21) 2021; 591
Hale (bib23) 2021; 10
Chen, Chin, Kenney, Tavares, Khan, Conway, Liu, Choudhary, Gertje, O'Connell (bib36) 2023; 615
Zhou, Yang, Wang, Hu, Zhang, Zhang, Si, Zhu, Li, Huang (bib1) 2020; 579
Pommerenke, Rand, Uphoff, Nagel, Zaborski, Hauer, Kaufmann, Meyer, Denkmann, Riese (bib25) 2021; 16
Winstone, Lista, Reid, Bouton, Pickering, Galao, Kerridge, Doores, Swanson, Neil (bib22) 2021; 95
Washington, Gangavarapu, Zeller, Bolze, Cirulli, Schiabor Barrett, Larsen, Anderson, White, Cassens (bib7) 2021; 184
Cele, Jackson, Khoury, Khan, Moyo-Gwete, Tegally, San, Cromer, Scheepers, Amoako (bib11) 2022; 602
Boehm, Kronig, Neher, Eckerle, Vetter, Kaiser (bib6) 2021; 27
Ye, Chiem, Park, Oladunni, Platt, Anderson, Almazan, de la Torre, Martinez-Sobrido (bib30) 2020; 11
Hou, Chiba, Halfmann, Ehre, Kuroda, Dinnon, Leist, Schäfer, Nakajima, Takahashi (bib5) 2020; 370
Puray-Chavez, LaPak, Schrank, Elliott, Bhatt, Agajanian, Jasuja, Lawson, Davis, Rothlauf (bib26) 2021; 36
Plante, Liu, Liu, Xia, Johnson, Lokugamage, Zhang, Muruato, Zou, Fontes-Garfias (bib3) 2021; 592
Hoffmann, Kleine-Weber, Schroeder, Krüger, Herrler, Erichsen, Schiergens, Herrler, Wu, Nitsche (bib35) 2020; 181
Lamers, Mykytyn, Breugem, Wang, Wu, Riesebosch, van den Doel, Schipper, Bestebroer, Wu, Haagmans (bib27) 2021; 10
Wurtz, Penant, Jardot, Duclos, La Scola (bib8) 2021; 40
Zaki, van Boheemen, Bestebroer, Osterhaus, Fouchier (bib10) 2012; 367
Ogando, Dalebout, Zevenhoven-Dobbe, Limpens, van der Meer, Caly, Druce, de Vries, Kikkert, Bárcena (bib19) 2020; 101
Uemura, Sasaki, Sanaki, Toba, Takahashi, Orba, Hall, Maenaka, Sawa, Sato (bib24) 2021; 11
Corman, Landt, Kaiser, Molenkamp, Meijer, Chu, Bleicker, Brünink, Schneider, Schmidt (bib37) 2020; 25
Dong, Du, Gardner (bib2) 2020; 20
Min, Talattof, Tsume, Stringer, Yu, Lim, Rosania (bib28) 2013; 30
Pohl, Busnadiego, Kufner, Glas, Karakus, Schmutz, Zaheri, Abela, Trkola, Huber (bib15) 2021; 19
Chen (10.1016/j.isci.2023.106634_bib36) 2023; 615
Washington (10.1016/j.isci.2023.106634_bib7) 2021; 184
Drosten (10.1016/j.isci.2023.106634_bib9) 2003; 348
Chen (10.1016/j.isci.2023.106634_bib34) 2021; 95
Torii (10.1016/j.isci.2023.106634_bib13) 2021; 35
Ye (10.1016/j.isci.2023.106634_bib30) 2020; 11
Corman (10.1016/j.isci.2023.106634_bib37) 2020; 25
Dong (10.1016/j.isci.2023.106634_bib2) 2020; 20
Davies (10.1016/j.isci.2023.106634_bib4) 2021; 372
Lau (10.1016/j.isci.2023.106634_bib17) 2020; 9
Hoffmann (10.1016/j.isci.2023.106634_bib35) 2020; 181
Peacock (10.1016/j.isci.2023.106634_bib20) 2021; 6
Davidson (10.1016/j.isci.2023.106634_bib16) 2020; 12
Suzuki (10.1016/j.isci.2023.106634_bib12) 2022; 603
Thi Nhu Thao (10.1016/j.isci.2023.106634_bib31) 2020; 582
Winstone (10.1016/j.isci.2023.106634_bib22) 2021; 95
Johnson (10.1016/j.isci.2023.106634_bib21) 2021; 591
Amarilla (10.1016/j.isci.2023.106634_bib14) 2021; 12
Wurtz (10.1016/j.isci.2023.106634_bib8) 2021; 40
Lamers (10.1016/j.isci.2023.106634_bib27) 2021; 10
Puray-Chavez (10.1016/j.isci.2023.106634_bib26) 2021; 36
Baczenas (10.1016/j.isci.2023.106634_bib33) 2021; 13
Hale (10.1016/j.isci.2023.106634_bib23) 2021; 10
Cele (10.1016/j.isci.2023.106634_bib11) 2022; 602
Zaki (10.1016/j.isci.2023.106634_bib10) 2012; 367
Feng (10.1016/j.isci.2023.106634_bib32) 2022; 14
Hou (10.1016/j.isci.2023.106634_bib5) 2020; 370
Zhou (10.1016/j.isci.2023.106634_bib1) 2020; 579
Plante (10.1016/j.isci.2023.106634_bib3) 2021; 592
Uemura (10.1016/j.isci.2023.106634_bib24) 2021; 11
Pommerenke (10.1016/j.isci.2023.106634_bib25) 2021; 16
Herrmann (10.1016/j.isci.2023.106634_bib29) 2021; 22
Pohl (10.1016/j.isci.2023.106634_bib15) 2021; 19
Liu (10.1016/j.isci.2023.106634_bib18) 2020; 94
Ogando (10.1016/j.isci.2023.106634_bib19) 2020; 101
Boehm (10.1016/j.isci.2023.106634_bib6) 2021; 27
Min (10.1016/j.isci.2023.106634_bib28) 2013; 30
References_xml – volume: 12
  start-page: 3431
  year: 2021
  ident: bib14
  article-title: A versatile reverse genetics platform for SARS-CoV-2 and other positive-strand RNA viruses
  publication-title: Nat. Commun.
– volume: 35
  start-page: 109014
  year: 2021
  ident: bib13
  article-title: Establishment of a reverse genetics system for SARS-CoV-2 using circular polymerase extension reaction
  publication-title: Cell Rep.
– volume: 591
  start-page: 293
  year: 2021
  end-page: 299
  ident: bib21
  article-title: Loss of furin cleavage site attenuates SARS-CoV-2 pathogenesis
  publication-title: Nature
– volume: 184
  start-page: 2587
  year: 2021
  end-page: 2594.e7
  ident: bib7
  article-title: Emergence and rapid transmission of SARS-CoV-2 B.1.1.7 in the United States
  publication-title: Cell
– volume: 95
  start-page: e02422-20
  year: 2021
  ident: bib22
  article-title: The polybasic cleavage site in SARS-CoV-2 spike modulates viral sensitivity to type I interferon and IFITM2
  publication-title: J. Virol.
– volume: 592
  start-page: 116
  year: 2021
  end-page: 121
  ident: bib3
  article-title: Spike mutation D614G alters SARS-CoV-2 fitness
  publication-title: Nature
– volume: 13
  start-page: 2434
  year: 2021
  ident: bib33
  article-title: Propagation of SARS-CoV-2 in calu-3 cells to eliminate mutations in the furin cleavage site of spike
  publication-title: Viruses
– volume: 27
  start-page: 1109
  year: 2021
  end-page: 1117
  ident: bib6
  article-title: Novel SARS-CoV-2 variants: the pandemics within the pandemic
  publication-title: Clin. Microbiol. Infect.
– volume: 20
  start-page: 533
  year: 2020
  end-page: 534
  ident: bib2
  article-title: An interactive web-based dashboard to track COVID-19 in real time
  publication-title: Lancet Infect. Dis.
– volume: 367
  start-page: 1814
  year: 2012
  end-page: 1820
  ident: bib10
  article-title: Isolation of a novel coronavirus from a man with pneumonia in Saudi Arabia
  publication-title: N. Engl. J. Med.
– volume: 370
  start-page: 1464
  year: 2020
  end-page: 1468
  ident: bib5
  article-title: SARS-CoV-2 D614G variant exhibits efficient replication ex vivo and transmission in vivo
  publication-title: Science
– volume: 11
  start-page: 5376
  year: 2021
  ident: bib24
  article-title: MRC5 cells engineered to express ACE2 serve as a model system for the discovery of antivirals targeting SARS-CoV-2
  publication-title: Sci. Rep.
– volume: 101
  start-page: 925
  year: 2020
  end-page: 940
  ident: bib19
  article-title: SARS-coronavirus-2 replication in Vero E6 cells: replication kinetics, rapid adaptation and cytopathology
  publication-title: J. Gen. Virol.
– volume: 10
  start-page: e66815
  year: 2021
  ident: bib27
  article-title: Human airway cells prevent SARS-CoV-2 multibasic cleavage site cell culture adaptation
  publication-title: Elife
– volume: 6
  start-page: 899
  year: 2021
  end-page: 909
  ident: bib20
  article-title: The furin cleavage site in the SARS-CoV-2 spike protein is required for transmission in ferrets
  publication-title: Nat. Microbiol.
– volume: 9
  start-page: 837
  year: 2020
  end-page: 842
  ident: bib17
  article-title: Attenuated SARS-CoV-2 variants with deletions at the S1/S2 junction
  publication-title: Emerg. Microbes Infect.
– volume: 12
  start-page: 68
  year: 2020
  ident: bib16
  article-title: Characterisation of the transcriptome and proteome of SARS-CoV-2 reveals a cell passage induced in-frame deletion of the furin-like cleavage site from the spike glycoprotein
  publication-title: Genome Med.
– volume: 348
  start-page: 1967
  year: 2003
  end-page: 1976
  ident: bib9
  article-title: Identification of a novel coronavirus in patients with severe acute respiratory syndrome
  publication-title: N. Engl. J. Med.
– volume: 94
  start-page: e00790-20
  year: 2020
  ident: bib18
  article-title: Identification of common deletions in the spike protein of severe acute respiratory syndrome coronavirus 2
  publication-title: J. Virol.
– volume: 372
  year: 2021
  ident: bib4
  article-title: Estimated transmissibility and impact of SARS-CoV-2 lineage B.1.1.7 in England
  publication-title: Science
– volume: 22
  start-page: 10188
  year: 2021
  ident: bib29
  article-title: Cloning of a passage-free SARS-CoV-2 genome and mutagenesis using red recombination
  publication-title: Int. J. Mol. Sci.
– volume: 40
  start-page: 477
  year: 2021
  end-page: 484
  ident: bib8
  article-title: Culture of SARS-CoV-2 in a panel of laboratory cell lines, permissivity, and differences in growth profile
  publication-title: Eur. J. Clin. Microbiol. Infect. Dis.
– volume: 10
  start-page: e69496
  year: 2021
  ident: bib23
  article-title: Avoiding culture shock with the SARS-CoV-2 spike protein
  publication-title: Elife
– volume: 11
  start-page: e02168-20
  year: 2020
  ident: bib30
  article-title: Rescue of SARS-CoV-2 from a single bacterial artificial chromosome
  publication-title: mBio
– volume: 36
  start-page: 109364
  year: 2021
  ident: bib26
  article-title: Systematic analysis of SARS-CoV-2 infection of an ACE2-negative human airway cell
  publication-title: Cell Rep.
– volume: 615
  start-page: 143
  year: 2023
  end-page: 150
  ident: bib36
  article-title: Spike and nsp6 are key determinants of SARS-CoV-2 Omicron BA.1 attenuation
  publication-title: Nature
– volume: 603
  start-page: 700
  year: 2022
  end-page: 705
  ident: bib12
  article-title: Attenuated fusogenicity and pathogenicity of SARS-CoV-2 Omicron variant
  publication-title: Nature
– volume: 19
  start-page: e3001006
  year: 2021
  ident: bib15
  article-title: SARS-CoV-2 variants reveal features critical for replication in primary human cells
  publication-title: PLoS Biol.
– volume: 582
  start-page: 561
  year: 2020
  end-page: 565
  ident: bib31
  article-title: Rapid reconstruction of SARS-CoV-2 using a synthetic genomics platform
  publication-title: Nature
– volume: 579
  start-page: 270
  year: 2020
  end-page: 273
  ident: bib1
  article-title: A pneumonia outbreak associated with a new coronavirus of probable bat origin
  publication-title: Nature
– volume: 30
  start-page: 2118
  year: 2013
  end-page: 2132
  ident: bib28
  article-title: The extracellular microenvironment explains variations in passive drug transport across different airway epithelial cell types
  publication-title: Pharm. Res. (N. Y.)
– volume: 602
  start-page: 654
  year: 2022
  end-page: 656
  ident: bib11
  article-title: Omicron extensively but incompletely escapes Pfizer BNT162b2 neutralization
  publication-title: Nature
– volume: 95
  start-page: e0086221
  year: 2021
  ident: bib34
  article-title: SARS-CoV-2 disrupts proximal elements in the JAK-STAT pathway
  publication-title: J. Virol.
– volume: 16
  start-page: e0255622
  year: 2021
  ident: bib25
  article-title: Identification of cell lines CL-14, CL-40 and CAL-51 as suitable models for SARS-CoV-2 infection studies
  publication-title: PLoS One
– volume: 14
  year: 2022
  ident: bib32
  article-title: Sequencing during times of change: evaluating SARS-CoV-2 clinical samples during the transition from the delta to Omicron wave
  publication-title: Viruses
– volume: 181
  start-page: 271
  year: 2020
  end-page: 280.e8
  ident: bib35
  article-title: SARS-CoV-2 cell entry depends on ACE2 and TMPRSS2 and is blocked by a clinically proven protease inhibitor
  publication-title: Cell
– volume: 25
  year: 2020
  ident: bib37
  article-title: Detection of 2019 novel coronavirus (2019-nCoV) by real-time RT-PCR
  publication-title: Euro Surveill.
– volume: 603
  start-page: 700
  year: 2022
  ident: 10.1016/j.isci.2023.106634_bib12
  article-title: Attenuated fusogenicity and pathogenicity of SARS-CoV-2 Omicron variant
  publication-title: Nature
  doi: 10.1038/s41586-022-04462-1
– volume: 95
  start-page: e0086221
  year: 2021
  ident: 10.1016/j.isci.2023.106634_bib34
  article-title: SARS-CoV-2 disrupts proximal elements in the JAK-STAT pathway
  publication-title: J. Virol.
  doi: 10.1128/JVI.00862-21
– volume: 6
  start-page: 899
  year: 2021
  ident: 10.1016/j.isci.2023.106634_bib20
  article-title: The furin cleavage site in the SARS-CoV-2 spike protein is required for transmission in ferrets
  publication-title: Nat. Microbiol.
  doi: 10.1038/s41564-021-00908-w
– volume: 367
  start-page: 1814
  year: 2012
  ident: 10.1016/j.isci.2023.106634_bib10
  article-title: Isolation of a novel coronavirus from a man with pneumonia in Saudi Arabia
  publication-title: N. Engl. J. Med.
  doi: 10.1056/NEJMoa1211721
– volume: 582
  start-page: 561
  year: 2020
  ident: 10.1016/j.isci.2023.106634_bib31
  article-title: Rapid reconstruction of SARS-CoV-2 using a synthetic genomics platform
  publication-title: Nature
  doi: 10.1038/s41586-020-2294-9
– volume: 184
  start-page: 2587
  year: 2021
  ident: 10.1016/j.isci.2023.106634_bib7
  article-title: Emergence and rapid transmission of SARS-CoV-2 B.1.1.7 in the United States
  publication-title: Cell
  doi: 10.1016/j.cell.2021.03.052
– volume: 40
  start-page: 477
  year: 2021
  ident: 10.1016/j.isci.2023.106634_bib8
  article-title: Culture of SARS-CoV-2 in a panel of laboratory cell lines, permissivity, and differences in growth profile
  publication-title: Eur. J. Clin. Microbiol. Infect. Dis.
  doi: 10.1007/s10096-020-04106-0
– volume: 19
  start-page: e3001006
  year: 2021
  ident: 10.1016/j.isci.2023.106634_bib15
  article-title: SARS-CoV-2 variants reveal features critical for replication in primary human cells
  publication-title: PLoS Biol.
  doi: 10.1371/journal.pbio.3001006
– volume: 14
  year: 2022
  ident: 10.1016/j.isci.2023.106634_bib32
  article-title: Sequencing during times of change: evaluating SARS-CoV-2 clinical samples during the transition from the delta to Omicron wave
  publication-title: Viruses
  doi: 10.3390/v14071408
– volume: 13
  start-page: 2434
  year: 2021
  ident: 10.1016/j.isci.2023.106634_bib33
  article-title: Propagation of SARS-CoV-2 in calu-3 cells to eliminate mutations in the furin cleavage site of spike
  publication-title: Viruses
  doi: 10.3390/v13122434
– volume: 372
  year: 2021
  ident: 10.1016/j.isci.2023.106634_bib4
  article-title: Estimated transmissibility and impact of SARS-CoV-2 lineage B.1.1.7 in England
  publication-title: Science
  doi: 10.1126/science.abg3055
– volume: 602
  start-page: 654
  year: 2022
  ident: 10.1016/j.isci.2023.106634_bib11
  article-title: Omicron extensively but incompletely escapes Pfizer BNT162b2 neutralization
  publication-title: Nature
  doi: 10.1038/s41586-021-04387-1
– volume: 181
  start-page: 271
  year: 2020
  ident: 10.1016/j.isci.2023.106634_bib35
  article-title: SARS-CoV-2 cell entry depends on ACE2 and TMPRSS2 and is blocked by a clinically proven protease inhibitor
  publication-title: Cell
  doi: 10.1016/j.cell.2020.02.052
– volume: 9
  start-page: 837
  year: 2020
  ident: 10.1016/j.isci.2023.106634_bib17
  article-title: Attenuated SARS-CoV-2 variants with deletions at the S1/S2 junction
  publication-title: Emerg. Microbes Infect.
  doi: 10.1080/22221751.2020.1756700
– volume: 12
  start-page: 68
  year: 2020
  ident: 10.1016/j.isci.2023.106634_bib16
  article-title: Characterisation of the transcriptome and proteome of SARS-CoV-2 reveals a cell passage induced in-frame deletion of the furin-like cleavage site from the spike glycoprotein
  publication-title: Genome Med.
  doi: 10.1186/s13073-020-00763-0
– volume: 25
  year: 2020
  ident: 10.1016/j.isci.2023.106634_bib37
  article-title: Detection of 2019 novel coronavirus (2019-nCoV) by real-time RT-PCR
  publication-title: Euro Surveill.
  doi: 10.2807/1560-7917.ES.2020.25.3.2000045
– volume: 36
  start-page: 109364
  year: 2021
  ident: 10.1016/j.isci.2023.106634_bib26
  article-title: Systematic analysis of SARS-CoV-2 infection of an ACE2-negative human airway cell
  publication-title: Cell Rep.
  doi: 10.1016/j.celrep.2021.109364
– volume: 591
  start-page: 293
  year: 2021
  ident: 10.1016/j.isci.2023.106634_bib21
  article-title: Loss of furin cleavage site attenuates SARS-CoV-2 pathogenesis
  publication-title: Nature
  doi: 10.1038/s41586-021-03237-4
– volume: 348
  start-page: 1967
  year: 2003
  ident: 10.1016/j.isci.2023.106634_bib9
  article-title: Identification of a novel coronavirus in patients with severe acute respiratory syndrome
  publication-title: N. Engl. J. Med.
  doi: 10.1056/NEJMoa030747
– volume: 101
  start-page: 925
  year: 2020
  ident: 10.1016/j.isci.2023.106634_bib19
  article-title: SARS-coronavirus-2 replication in Vero E6 cells: replication kinetics, rapid adaptation and cytopathology
  publication-title: J. Gen. Virol.
  doi: 10.1099/jgv.0.001453
– volume: 22
  start-page: 10188
  year: 2021
  ident: 10.1016/j.isci.2023.106634_bib29
  article-title: Cloning of a passage-free SARS-CoV-2 genome and mutagenesis using red recombination
  publication-title: Int. J. Mol. Sci.
  doi: 10.3390/ijms221910188
– volume: 30
  start-page: 2118
  year: 2013
  ident: 10.1016/j.isci.2023.106634_bib28
  article-title: The extracellular microenvironment explains variations in passive drug transport across different airway epithelial cell types
  publication-title: Pharm. Res. (N. Y.)
  doi: 10.1007/s11095-013-1069-5
– volume: 95
  start-page: e02422-20
  year: 2021
  ident: 10.1016/j.isci.2023.106634_bib22
  article-title: The polybasic cleavage site in SARS-CoV-2 spike modulates viral sensitivity to type I interferon and IFITM2
  publication-title: J. Virol.
  doi: 10.1128/JVI.02422-20
– volume: 20
  start-page: 533
  year: 2020
  ident: 10.1016/j.isci.2023.106634_bib2
  article-title: An interactive web-based dashboard to track COVID-19 in real time
  publication-title: Lancet Infect. Dis.
  doi: 10.1016/S1473-3099(20)30120-1
– volume: 10
  start-page: e66815
  year: 2021
  ident: 10.1016/j.isci.2023.106634_bib27
  article-title: Human airway cells prevent SARS-CoV-2 multibasic cleavage site cell culture adaptation
  publication-title: Elife
  doi: 10.7554/eLife.66815
– volume: 592
  start-page: 116
  year: 2021
  ident: 10.1016/j.isci.2023.106634_bib3
  article-title: Spike mutation D614G alters SARS-CoV-2 fitness
  publication-title: Nature
  doi: 10.1038/s41586-020-2895-3
– volume: 11
  start-page: 5376
  year: 2021
  ident: 10.1016/j.isci.2023.106634_bib24
  article-title: MRC5 cells engineered to express ACE2 serve as a model system for the discovery of antivirals targeting SARS-CoV-2
  publication-title: Sci. Rep.
  doi: 10.1038/s41598-021-84882-7
– volume: 27
  start-page: 1109
  year: 2021
  ident: 10.1016/j.isci.2023.106634_bib6
  article-title: Novel SARS-CoV-2 variants: the pandemics within the pandemic
  publication-title: Clin. Microbiol. Infect.
  doi: 10.1016/j.cmi.2021.05.022
– volume: 579
  start-page: 270
  year: 2020
  ident: 10.1016/j.isci.2023.106634_bib1
  article-title: A pneumonia outbreak associated with a new coronavirus of probable bat origin
  publication-title: Nature
  doi: 10.1038/s41586-020-2012-7
– volume: 370
  start-page: 1464
  year: 2020
  ident: 10.1016/j.isci.2023.106634_bib5
  article-title: SARS-CoV-2 D614G variant exhibits efficient replication ex vivo and transmission in vivo
  publication-title: Science
  doi: 10.1126/science.abe8499
– volume: 35
  start-page: 109014
  year: 2021
  ident: 10.1016/j.isci.2023.106634_bib13
  article-title: Establishment of a reverse genetics system for SARS-CoV-2 using circular polymerase extension reaction
  publication-title: Cell Rep.
  doi: 10.1016/j.celrep.2021.109014
– volume: 12
  start-page: 3431
  year: 2021
  ident: 10.1016/j.isci.2023.106634_bib14
  article-title: A versatile reverse genetics platform for SARS-CoV-2 and other positive-strand RNA viruses
  publication-title: Nat. Commun.
  doi: 10.1038/s41467-021-23779-5
– volume: 16
  start-page: e0255622
  year: 2021
  ident: 10.1016/j.isci.2023.106634_bib25
  article-title: Identification of cell lines CL-14, CL-40 and CAL-51 as suitable models for SARS-CoV-2 infection studies
  publication-title: PLoS One
  doi: 10.1371/journal.pone.0255622
– volume: 94
  start-page: e00790-20
  year: 2020
  ident: 10.1016/j.isci.2023.106634_bib18
  article-title: Identification of common deletions in the spike protein of severe acute respiratory syndrome coronavirus 2
  publication-title: J. Virol.
  doi: 10.1128/JVI.00790-20
– volume: 11
  start-page: e02168-20
  year: 2020
  ident: 10.1016/j.isci.2023.106634_bib30
  article-title: Rescue of SARS-CoV-2 from a single bacterial artificial chromosome
  publication-title: mBio
  doi: 10.1128/mBio.02168-20
– volume: 615
  start-page: 143
  year: 2023
  ident: 10.1016/j.isci.2023.106634_bib36
  article-title: Spike and nsp6 are key determinants of SARS-CoV-2 Omicron BA.1 attenuation
  publication-title: Nature
  doi: 10.1038/s41586-023-05697-2
– volume: 10
  start-page: e69496
  year: 2021
  ident: 10.1016/j.isci.2023.106634_bib23
  article-title: Avoiding culture shock with the SARS-CoV-2 spike protein
  publication-title: Elife
  doi: 10.7554/eLife.69496
SSID ssj0002002496
Score 2.2797506
Snippet A simple and robust cell culture system is essential for generating authentic SARS-CoV-2 stocks for evaluation of viral pathogenicity, screening of antiviral...
A simple and robust cell culture system is essential for generating authentic SARS-CoV-2 stocks required for evaluation of viral pathogenicity, screening of...
SourceID doaj
pubmedcentral
proquest
pubmed
crossref
elsevier
SourceType Open Website
Open Access Repository
Aggregation Database
Index Database
Enrichment Source
Publisher
StartPage 106634
SubjectTerms Methodology in biological sciences
Virology
Title Cell culture systems for isolation of SARS-CoV-2 clinical isolates and generation of recombinant virus
URI https://dx.doi.org/10.1016/j.isci.2023.106634
https://www.ncbi.nlm.nih.gov/pubmed/37095858
https://www.proquest.com/docview/2806071972
https://pubmed.ncbi.nlm.nih.gov/PMC10083141
https://doaj.org/article/4ef512ed285e4127ae84089843aedd7f
Volume 26
hasFullText 1
inHoldings 1
isFullTextHit
isPrint
link http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwrV1Lb9QwELZQT1wQqDzCS0bihgx1PI6dY1lRVUhwoBT1Zjn2BLZasqjb5fd3bCerDUjlwjVxHvaM_X0jj79h7HWUnW8iWKHBtwKw9aI9aqIIGZFqHTDLNX363Jyew8cLfbFX6ivlhBV54DJw7wB7wiSMtdUIsjYeKSSxrQXlMUbTp9WXMG8vmLrM22tJCi9XltMpJ4hcczwxU5K70onXt6lyOF0gzIUZKmXx_hk4_U0-_8yh3AOlk_vs3sgm-XHpxQN2B4dD1i9wteJFUgN5kWrecCKnfEmOli3B1z0_O_5yJhbrb6Lm0_HIsQFuuB8i_54VqafmKXL-2eW8Gf57ebXdPGTnJx--Lk7FWE5BBFAGRNNQLBVrYxBUB9qEpgkKeh9RtgAEZIT-qAnVoj7yKijvY6CRDhSQeNnRyvCIHQzrAZ8w3ilP1FD1RvUAqDofvNUeWhuIqwdpKian4XRh1BpPJS9Wbkoqu3TJBC6ZwBUTVOzN7plfRWnj1tbvk5V2LZNKdr5AvuNG33H_8p2K6cnGbiQchUjQq5a3fvzV5BCOZmPaYvEDrrcbl_apibS1pq7Y4-Igu19Uhuis1bZiduY6sz7M7wzLH1nxW-aCcCCf_o9eP2N3U19SCoRsn7OD66stviBmdd29zJPoBtXAHvA
linkProvider Directory of Open Access Journals
openUrl ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Cell+culture+systems+for+isolation+of+SARS-CoV-2+clinical+isolates+and+generation+of+recombinant+virus&rft.jtitle=iScience&rft.au=Chen%2C+Da-Yuan&rft.au=Turcinovic%2C+Jacquelyn&rft.au=Feng%2C+Shuchen&rft.au=Kenney%2C+Devin+J&rft.date=2023-05-19&rft.issn=2589-0042&rft.eissn=2589-0042&rft.volume=26&rft.issue=5&rft.spage=106634&rft_id=info:doi/10.1016%2Fj.isci.2023.106634&rft.externalDBID=NO_FULL_TEXT
thumbnail_l http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=2589-0042&client=summon
thumbnail_m http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=2589-0042&client=summon
thumbnail_s http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=2589-0042&client=summon