Sensitivity comparison for detection of respiratory bovine coronaviruses in nasal samples from feedlot cattle by ELISA and isolation with the G clone of HRT-18 cells

• Published in: Journal of veterinary diagnostic investigation Vol. 11; no. 1; pp. 15 - 19
• Main Authors: Silva, M.R. da, O'Reilly, K.L, Lin, X.Q, Stine, L, Storz, J
• Format: Journal Article
• Language: English
• Published: United States 1999
• Subjects: Animals; antigen detection; Cattle; Cattle Diseases - diagnosis; Cattle Diseases - virology; Clone Cells; Coronavirus; Coronavirus Infections - diagnosis; Coronavirus Infections - veterinary; Coronavirus, Bovine - isolation & purification; enzyme-linked immunosorbent assay; Enzyme-Linked Immunosorbent Assay - methods; Humans; isolation; mucosa; nasal mucosa; Nasal Mucosa - virology; Rectal Neoplasms; Reproducibility of Results; respiratory tract diseases; Respiratory Tract Infections - diagnosis; Respiratory Tract Infections - veterinary; Respiratory Tract Infections - virology; Sensitivity and Specificity; Specimen Handling - methods; Specimen Handling - veterinary; Tumor Cells, Cultured; viral antigens

A monoclonal antibody-based capture enzyme-linked immunosorbent assay (ELISA) was developed to detect respiratory bovine coronavirus (RBCV) antigens in nasal swabs collected from cattle showing signs of respiratory tract disease following shipping. These samples had been previously tested for RBCV b...