Calcium requirements during mitotic cdc2 kinase activation and cyclin degradation in Xenopus egg extracts

• Published in: Journal of cell science Vol. 108 ( Pt 11); no. 11; pp. 3557 - 3568
• Main Authors: Lindsay, H D, Whitaker, M J, Ford, C C
• Format: Journal Article
• Language: English
• Published: England 01.11.1995
• Subjects: Animals; Calcium - metabolism; Calcium - physiology; Calcium-Calmodulin-Dependent Protein Kinase Type 2; Calcium-Calmodulin-Dependent Protein Kinases - pharmacology; CDC2 Protein Kinase - metabolism; Cell Extracts - physiology; Chelating Agents - pharmacology; Cyclins - metabolism; Egtazic Acid - analogs & derivatives; Egtazic Acid - pharmacology; Enzyme Activation - drug effects; Female; Interphase - physiology; Maturation-Promoting Factor - metabolism; Mitosis - physiology; Ovum - cytology; Ovum - enzymology; Peptides - pharmacology; Phosphorylation; Prophase - physiology; Xenopus

Activation of p34cdc2 kinase is essential for entry into mitosis while subsequent deactivation and cyclin degradation are associated with exit. In Xenopus embryos, both of these phases are regulated by post-translation modifications and occur spontaneously on incubation of extracts prepared late in...