Inhibitory Effects of MicroRNA-34a on Cell Migration and Invasion of Invasive Urothelial Bladder Carcinoma by Targeting Notch1

• Published in: Journal of Huazhong University of Science and Technology. Medical sciences Vol. 32; no. 3; pp. 375 - 382
• Main Author: 张超 姚志勇 朱鸣阳 马鑫 史涛坪 李宏召 王保军 欧阳金芝 张旭
• Format: Journal Article
• Language: English
• Published: Heidelberg Huazhong University of Science and Technology 01.06.2012
• Subjects: Adult; Aged; Cell Movement - genetics; Down-Regulation - genetics; Female; Gene Targeting; Humans; Male; Medicine; Medicine & Public Health; MicroRNAs - genetics; Middle Aged; Neoplasm Invasiveness; Receptor, Notch1 - physiology; RT-PCR检测; Transfection; Tumor Cells, Cultured; Urinary Bladder Neoplasms - pathology; Urinary Bladder Neoplasms - physiopathology; Western印迹法; 上皮; 侵袭; 抑制效应; 浸润性; 细胞迁移; 膀胱癌; migration; microRNA-34a; urothelial carcinoma; invasion; Notch1
• ISSN: 1672-0733; 1993-1352
• DOI: 10.1007/s11596-012-0065-z

MicroRNAs （miRNAs or miRs） are a class of short, non-coding RNAs that participate in various oncological processes. This study aims to explore the roles of microRNA-34a （miR-34a） in invasive urothelial bladder carcinoma. miR-34a was transfected into bladder cancer cell lines 253J and J82. The miR-34a expression levels in tissues and cells were detected by using qRT-PCR. The Notch1 expression was detected by qRT-PCR and Western blotting. Cell migratory and invasive abilities were measured by Transwell chamber assay. Bioinformatics and luciferase assay were performed to predict and analyze the binding sites between miRNA-34a and Notch1. It was found that there was aberrant expression of miR-34a in bladder cancer tissues. Moreover, we revealed that ectopic expression of miR-34a suppressed cell migration and invasion, while forced expression of Notch1 increased cell migratory and invasive abilities. Finally, we observed that miR-34a transfection significantly down-regulated luciferase activity and reduced the mRNA and protein levels of Notch1. Our study concluded that microRNA-34a antagonizes Notch1 and inhibits cell migration and invasion of bladder cancer cells, which indicates the tumor-suppressive function of microRNA-34a in bladder cancer.