Nanopore sequencing technology and tools for genome assembly: computational analysis of the current state, bottlenecks and future directions

• Published in: Briefings in bioinformatics Vol. 20; no. 4; pp. 1542 - 1559
• Main Authors: Senol Cali, Damla, Kim, Jeremie S, Ghose, Saugata, Alkan, Can, Mutlu, Onur
• Format: Journal Article
• Language: English
• Published: England Oxford University Press 19.07.2019; Oxford Publishing Limited (England)
• Subjects: Accuracy; Animals; Assembly; Chromosome Mapping; Computational Biology; Errors; Escherichia coli - genetics; Genome, Bacterial; Genomes; Genomics - methods; Genomics - statistics & numerical data; Genomics - trends; Humans; Nanopore Sequencing - methods; Nanopore Sequencing - statistics & numerical data; Nanopore Sequencing - trends; Nucleotide sequence; Polishing; Review; Sequence analysis; Sequence Analysis, DNA; Software; Tradeoffs; Whole genome sequencing; assembly; nanopore sequencing; genome sequencing; mapping; genome analysis

Abstract Nanopore sequencing technology has the potential to render other sequencing technologies obsolete with its ability to generate long reads and provide portability. However, high error rates of the technology pose a challenge while generating accurate genome assemblies. The tools used for nan...