Visualization of Nuclease- and Serum-Mediated Chromatin Degradation with DNA–Histone Mesostructures

• Published in: International journal of molecular sciences Vol. 24; no. 4; p. 3222
• Main Authors: Wasielewski, Midori L., Nguyen, Katherine, Yalavarthi, Srilakshmi, Ekbote, Pallavi, Weerappuli, Priyan D., Knight, Jason S., Takayama, Shuichi
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 06.02.2023; MDPI
• Subjects: Automation; Chromatin; Chromatin - metabolism; Circular DNA; Deoxyribonuclease I - metabolism; DNA - metabolism; DNA binding proteins; Ethylenediaminetetraacetic acid; Extracellular Traps - metabolism; Histones - metabolism; Humans; Lupus; Neutrophils; Neutrophils - metabolism; Nucleases; Physiological aspects; Scientific equipment and supplies industry; United States; Germany; Missouri; Michigan; Ann Arbor Michigan; United States > US; NETs in autoimmunity; chromatin degradation; neutrophil extracellular traps (NETs)

This study analyzed the nuclease- and serum-driven degradation of millimeter-scale, circular DNA–histone mesostructures (DHMs). DHMs are bioengineered chromatin meshes of defined DNA and histone compositions designed as minimal mimetics of physiological extracellular chromatin structures, such as ne...