DNA fragmentation and NAD depletion. Their relation to the turnover of endogenous mono(ADP-ribosyl) and poly(ADP-ribosyl) proteins

• Published in: The Journal of biological chemistry Vol. 257; no. 21; pp. 12872 - 12877
• Main Authors: Wielckens, K, Schmidt, A, George, E, Bredehorst, R, Hilz, H
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 10.11.1982; American Society for Biochemistry and Molecular Biology
• Subjects: Adenosine Diphosphate Ribose - metabolism; ADP Ribose Transferases; Animals; Carcinoma, Ehrlich Tumor - metabolism; DNA, Neoplasm - metabolism; Kinetics; Mice; NAD - metabolism; Nucleoside Diphosphate Sugars - metabolism; Poly Adenosine Diphosphate Ribose - metabolism; Poly(ADP-ribose) Polymerases - metabolism; Triaziquone - pharmacology

Treatment of Ehrlich ascites tumor cells with the trifunctional alkylating agent 2,3-5-tris(ethyleneimino)benzoquinone-1,4 (triaziquonum) led to rapid fragmentation of DNA and depletion of NAD while poly(ADP-ribose) synthetase activity showed a retarded increase. Poly(ADP-ribosyl) residues in treate...